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Protein-Containing ...
Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films
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- Isaksson, Simon, 1988 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden.,Department of Chemistry and Chemical Engineering, Chalmers University of Technology , SE-41296 Gothenburg, Sweden
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- Watkins, E. B. (författare)
- Los Alamos National Laboratory,Los Alamos Natl Lab, Mat Phys & Applicat Div, MPA 11, Los Alamos, NM 87545 USA.,Materials Physics and Application Division, MPA-11, Los Alamos National Laboratory , Los Alamos, New Mexico 87545, United States
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- Browning, Kathryn L. (författare)
- Uppsala universitet,Institutionen för farmaci,Uppsala University,Department of Pharmacy, Uppsala University , SE-75123 Uppsala, Sweden
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- Lind, Tania Kjellerup (författare)
- Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Cárdenas, Marité (författare)
- Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Hedfalk, Kristina, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Höök, Fredrik, 1966 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Appl Phys, SE-41296 Gothenburg, Sweden.,Department of Applied Physics, Chalmers University of Technology , SE-41296 Gothenburg, Sweden
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- Andersson, Martin, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden.,Department of Chemistry and Chemical Engineering, Chalmers University of Technology , SE-41296 Gothenburg, Sweden
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Chalmers tekniska högskola Chalmers, Dept Chem & Chem Engn, SE-41296 Gothenburg, Sweden (creator_code:org_t)
- 2016-11-29
- 2017
- Engelska.
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6992 .- 1530-6984. ; 17:1, s. 476-485
- Relaterad länk:
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http://dx.doi.org/10...
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https://www.osti.gov...
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https://research.cha...
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https://research.cha...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Proteins are key components in a multitude of biological processes, of which the functions carried out by transmembrane (membrane-spanning) proteins are especially demanding for investigations. This is because this class of protein needs to be incorporated into a lipid bilayer representing its native environment, and in addition, many experimental conditions also require a solid support for stabilization and analytical purposes. The solid support substrate may, however, limit the protein functionality due to protein material interactions and a lack of physical space. We have in this work tailored the pore size and pore ordering of a mesoporous silica thin film to match the native cell-membrane arrangement of the transmembrane protein human aquaporin 4 (hAQP4). Using neutron reflectivity (NR), we provide evidence of how substrate pores host the bulky water-soluble domain of hAQP4, which is shown to extend 7.2 nm into the pores of the substrate. Complementary surface analytical tools, including quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy, revealed successful protein-containing supported lipid bilayer (pSLB) formation on mesoporous silica substrates, whereas pSLB formation was hampered on nonporous silica. Additionally, electron microscopy (TEM and SEM), light scattering (DLS and stopped-flow), and small-angle X-ray scattering (SAXS) were employed to provide a comprehensive characterization of this novel hybrid organic-inorganic interface, the tailoring of which is likely to be generally applicable to improve the function and stability of a broad range of membrane proteins containing water-soluble domains.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Membrane protein
- Lipid bilayer
- membranes
- titania
- Aquaporin
- conductance
- angstrom
- Neutron reflectivity
- Liposome
- Silica
- vesicle fusion
- channel
- Aquaporin; Lipid bilayer; Liposome; Membrane protein; Neutron reflectivity; Silica
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Isaksson, Simon, ...
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Watkins, E. B.
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Browning, Kathry ...
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Lind, Tania Kjel ...
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Cárdenas, Marité
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Hedfalk, Kristin ...
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Höök, Fredrik, 1 ...
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Andersson, Marti ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Kemiteknik
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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NATURVETENSKAP
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och Fysik
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och Den kondenserade ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Materialkemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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Nano Letters
- Av lärosätet
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Chalmers tekniska högskola
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Uppsala universitet
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Malmö universitet
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Göteborgs universitet