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Tension induces a b...
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Bosaeus, Niklas,1982Chalmers tekniska högskola,Chalmers University of Technology
(författare)
Tension induces a base-paired overstretched DNA conformation
- Artikel/kapitelEngelska2012
Förlag, utgivningsår, omfång ...
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2012-09-04
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Proceedings of the National Academy of Sciences,2012
Nummerbeteckningar
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LIBRIS-ID:oai:research.chalmers.se:19125d1f-2522-4676-9dc7-af6296a916f4
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https://research.chalmers.se/publication/165605URI
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https://doi.org/10.1073/pnas.1213172109DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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Mixed-sequence DNA molecules undergo mechanical overstretching by approximately 70% at 60-70 pN. Since its initial discovery 15 y ago, a debate has arisen as to whether the molecule adopts a new form [Cluzel P, et al. (1996) Science 271: 792-794; Smith SB, Cui Y, Bustamante C (1996) Science 271: 795-799], or simply denatures under tension [van Mameren J, et al. (2009) Proc Natl Acad Sci USA 106: 18231-18236]. Here, we resolve this controversy by using optical tweezers to extend small 60-64 bp single DNA duplex molecules whose base content can be designed at will. We show that when AT content is high (70%), a force-induced denaturation of the DNA helix ensues at 62 pN that is accompanied by an extension of the molecule of approximately 70%. By contrast, GC-rich sequences (60% GC) are found to undergo a reversible overstretching transition into a distinct form that is characterized by a 51% extension and that remains base-paired. For the first time, results proving the existence of a stretched basepaired form of DNA can be presented. The extension observed in the reversible transition coincides with that produced on DNA by binding of bacterial RecA and human Rad51, pointing to its possible relevance in homologous recombination.
Ämnesord och genrebeteckningar
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NATURVETENSKAP Kemi hsv//swe
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NATURAL SCIENCES Chemical Sciences hsv//eng
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stranded-dna
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mechanism of recombination
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single molecule
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molecule
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double helix
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mechanical properties of DNA
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acid
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DNA stretching
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dichroism
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terminal alkynes
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complexes
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elongated DNA
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thermodynamics
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structural transitions
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force
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linear
Biuppslag (personer, institutioner, konferenser, titlar ...)
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El-Sagheer, AfafUniversity of Southampton
(författare)
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Brown, T.University of Southampton
(författare)
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Smith, S. B.University of California
(författare)
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Åkerman, Björn,1957Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)baa
(författare)
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Bustamante, C.University of California
(författare)
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Nordén, Bengt,1945Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)norden
(författare)
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Chalmers tekniska högskolaUniversity of Southampton
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences109:38, s. 15179-151840027-84241091-6490
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Bosaeus, Niklas, ...
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El-Sagheer, Afaf
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Brown, T.
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Smith, S. B.
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Åkerman, Björn, ...
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Bustamante, C.
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Nordén, Bengt, 1 ...
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