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Analytical performa...
Analytical performance of a standardized kit for mass spectrometry-based measurements of human glycosaminoglycans
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Tamburro, Davide (författare)
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- Bratulic, Sinisa, 1981 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Abou Shameh, Souad (författare)
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- Soni, Nikulkumar, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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Bacconi, Andrea (författare)
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- Maccari, F. (författare)
- Universita Degli Studi Di Modena E Reggio Emilia,University of Modena and Reggio Emilia
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- Galeotti, F. (författare)
- Universita Degli Studi Di Modena E Reggio Emilia,University of Modena and Reggio Emilia
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Mattsson, Karin (författare)
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- Volpi, N. (författare)
- Universita Degli Studi Di Modena E Reggio Emilia,University of Modena and Reggio Emilia
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- Nielsen, Jens B, 1962 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII)
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- Gatto, Francesco, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
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Ingår i: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 1177
- Relaterad länk:
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https://research.cha... (primary) (free)
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Glycosaminoglycans (GAGs) are long linear sulfated polysaccharides implicated in processes linked to disease development such as mucopolysaccharidosis, respiratory failure, cancer, and viral infections, thereby serving as potential biomarkers. A successful clinical translation of GAGs as biomarkers depends on the availability of standardized GAG measurements. However, owing to the analytical complexity associated with the quantification of GAG concentration and structural composition, a standardized method to simultaneously measure multiple GAGs is missing. In this study, we sought to characterize the analytical performance of a ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UHPLC-MS/MS)-based kit for the quantification of 17 free GAG disaccharides. The kit showed acceptable linearity, selectivity and specificity, accuracy and precision, and analyte stability in the absolute quantification of 15 disaccharides. In native human samples, here using urine as a reference matrix, the analytical performance of the kit was acceptable for the quantification of CS disaccharides. Intra- and inter-laboratory tests performed in an external laboratory demonstrated robust reproducibility of GAG measurements showing that the kit was acceptably standardized. In conclusion, these results indicated that the UHPLC-MS/MS kit was standardized for the simultaneous measurement of free GAG disaccharides allowing for comparability of measurements and enabling translational research.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- UHPLC-MSMS
- Oligosaccharides
- Glycosaminoglycans
- Analytical validation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Tamburro, Davide
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Bratulic, Sinisa ...
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Abou Shameh, Sou ...
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Soni, Nikulkumar ...
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Bacconi, Andrea
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Maccari, F.
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visa fler...
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Galeotti, F.
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Mattsson, Karin
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Volpi, N.
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Nielsen, Jens B, ...
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Gatto, Francesco ...
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Medicinteknik
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och Medicinsk labora ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomedicinsk lab ...
- Artiklar i publikationen
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Journal of Chrom ...
- Av lärosätet
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Chalmers tekniska högskola