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Template-directed o...
Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry
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- Kumar, R. (författare)
- University of Southampton
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- El-Sagheer, Afaf (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Tumpane, John, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Lincoln, Per, 1958 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Wilhelmsson, Marcus, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Brown, T. (författare)
- University of Southampton
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(creator_code:org_t)
- 2007-05-09
- 2007
- Engelska.
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 129:21, s. 6859-6864
- Relaterad länk:
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http://dx.doi.org/10...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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