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Impact of forced fa...
Impact of forced fatty acid synthesis on metabolism and physiology of Saccharomyces cerevisiae
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- Gossing, Michael, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Smialowska, Agata, 1977 (författare)
- National Bioinformatics Infrastructure Sweden (NBIS),Chalmers tekniska högskola,Chalmers University of Technology
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- Nielsen, Jens B, 1962 (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2018-08-29
- 2018
- Engelska.
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Ingår i: FEMS Yeast Research. - : Oxford University Press (OUP). - 1567-1356 .- 1567-1364. ; 18:8
- Relaterad länk:
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https://academic.oup...
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visa fler...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Nutrient sensing and signaling controls the cellular response to extracellular nutrients and intracellular metabolites. Nutrient-dependent regulation of metabolism ensures balanced energy production and expenditure. We show that disturbing energy balance by forcing fatty acid synthesis has profound impact on metabolism and physiology of the yeast cell. In addition to an expected increase in storage lipids, we observed increased β-oxidation and reduced amino acid biosynthesis, indicating increased activity of nutrient-sensitive kinase Snf1p. We also observed increased sensitivity to rapamycin as well as decreased ribosome biogenesis and translation, indicating reduced activity of nutrient-sensitive kinase target of rapamycin complex 1. Additionally, we detected increased levels of oxidative stress and lower levels of amino acids. This study provides detailed insight into cellular resource redistribution in response to forced fatty acid synthesis and enables optimized engineering of microbial lipid production.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- lipid metabolism
- TORC1
- cell wall integrity signaling pathway
- Snf1p/AMPK
- amino acid metabolism
- reactive oxygen species
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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