Sökning: onr:"swepub:oai:research.chalmers.se:35e4ff60-bc61-4e9d-b84a-6a5f7ad73a50" >
Phosphoproteome Stu...
Phosphoproteome Study of Escherichia coli Devoid of Ser/Thr Kinase YeaG During the Metabolic Shift From Glucose to Malate
-
- Sultan, Abida (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark
-
- Jers, Carsten (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark
-
- Ganief, Tariq A. (författare)
- Eberhard Karls Universität Tübingen,Eberhard Karls University of Tübingen
-
visa fler...
-
- Shi, Lei, 1981 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Senissar, Meriem (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark
-
- Kohler, Julie Bonne (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark
-
- Macek, Boris (författare)
- Eberhard Karls Universität Tübingen,Eberhard Karls University of Tübingen
-
- Mijakovic, Ivan, 1975 (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Chalmers tekniska högskola,Chalmers University of Technology
-
visa färre...
-
(creator_code:org_t)
- 2021-04-06
- 2021
- Engelska.
-
Ingår i: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 12
- Relaterad länk:
-
https://research.cha... (primary) (free)
-
visa fler...
-
https://www.frontier...
-
https://research.cha...
-
https://doi.org/10.3...
-
https://research.cha...
-
visa färre...
Abstract
Ämnesord
Stäng
- Understanding phosphorylation-mediated regulation of metabolic enzymes, pathways, and cell phenotypes under metabolic shifts represents a major challenge. The kinases associated with most phosphorylation sites and the link between phosphorylation and enzyme activity remain unknown. In this study, we performed stable isotope labeling by amino acids in cell culture (SILAC)-based proteome and phosphoproteome analysis of Escherichia coli Delta yeaG, a strain lacking a poorly characterized serine/threonine kinase YeaG, to decipher kinase-substrate interactions and the effects on metabolic phenotype during shifts from glucose to malate. The starting point of our analysis was the identification of physiological conditions under which Delta yeaG exhibits a clear phenotype. By metabolic profiling, we discovered that Delta yeaG strain has a significantly shorter lag phase than the wild type during metabolic shift from glucose to malate. Under those conditions, our SILAC analysis revealed several proteins that were differentially phosphorylated in the Delta yeaG strain. By focusing on metabolic enzymes potentially involved in central carbon metabolism, we narrowed down our search for putative YeaG substrates and identified isocitrate lyase AceA as the direct substrate of YeaG. YeaG was capable of phosphorylating AceA in vitro only in the presence of malate, suggesting that this phosphorylation event is indeed relevant for glucose to malate shift. There is currently not enough evidence to firmly establish the exact mechanism of this newly observed regulatory phenomenon. However, our study clearly exemplifies the usefulness of SILAC-based approaches in identifying proteins kinase substrates, when applied in physiological conditions relevant for the activity of the protein kinase in question.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- SILAC
- metabolic adaptation
- phosphoproteome
- kinase-substrate relationship
- protein kinase
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Sultan, Abida
-
Jers, Carsten
-
Ganief, Tariq A.
-
Shi, Lei, 1981
-
Senissar, Meriem
-
Kohler, Julie Bo ...
-
visa fler...
-
Macek, Boris
-
Mijakovic, Ivan, ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Cellbiologi
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
- Artiklar i publikationen
-
Frontiers in Mic ...
- Av lärosätet
-
Chalmers tekniska högskola