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Biochemical and str...
Biochemical and structural investigation of the CE15 family: Glucuronoyl esterases acting on recalcitrant biomass
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- Mazurkewich, Scott, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Arnling Bååth, Jenny, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Knudsen, Rasmus Meland (författare)
- Köpenhamns universitet,University of Copenhagen
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visa fler...
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- Poulsen, Jens-Christian N (författare)
- Köpenhamns universitet,University of Copenhagen
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- Olsson, Lisbeth, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Lo Leggio, Leila (författare)
- Köpenhamns universitet,University of Copenhagen
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- Larsbrink, Johan, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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visa färre...
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
- Relaterad länk:
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https://research.cha...
Abstract
Ämnesord
Stäng
- Glucuronoyl esterases (GEs) are a relatively new class of enzymes which cleave ester linkages between lignin and glucuronoxylan. GEs have been identified in many biomass-degrading microbes and are now classified into the Carbohydrate Esterase Family 15 (CE15). The CE15 family is diverse (as low as 20% sequence identity), however to-date only a few GEs from a small clade of the CE15 phylogenetic tree have been biochemically characterized and only two protein structures have been solved. To investigate the diversity of CE15 members, we have studied a broad range of proteins from across the phylogenetic tree. Enzymes were biochemically characterized and three-dimensional structures for two of the enzymes were solved. Analysis of the structures suggest possible binding sites for lignin fragments and xylooligosaccharides that have not been previously reported. Investigations into the molecular determinants supporting the potential binding sites is being pursued.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- lignin
- biomass
- glucuronoyl esterase
- glucuronoxylan
- CE15
- crystallography
- Carbohydrate Esterase
Publikations- och innehållstyp
- kon (ämneskategori)
- vet (ämneskategori)