Sökning: onr:"swepub:oai:research.chalmers.se:4a9a7680-6351-478d-b885-4e2aeb982473" >
Visualization of β-...
Visualization of β-carotene and starch granules in plant cells using CARS and SHG microscopy
-
- Brackmann, Christian, 1973 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Bengtsson, Anton, 1979 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Alminger, Marie, 1957 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
visa fler...
-
- Svanberg, Ulf, 1945 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Enejder, Annika, 1969 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
visa färre...
-
(creator_code:org_t)
- 2010-08-28
- 2010
- Engelska.
-
Ingår i: Journal of Raman Spectroscopy. - : Wiley. - 0377-0486 .- 1097-4555. ; 42:4, s. 586-592
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://doi.org/10.1...
-
https://research.cha...
-
visa färre...
Abstract
Ämnesord
Stäng
- Information on the content and bioavailability of provitamin A carotenoids, such as beta-carotene, in plant foods is of great interest due to the widespread vitamin A deficiency in developing countries. While the amount of beta-carotene can readily be quantified with analytical techniques, there is limited information on beta-carotene morphology in native plant materials. Here, we introduce nonlinear microscopy for three-dimensional, label-free imaging of carotenoids in fresh and thermally treated plant tissues, providing quantitative information at single-aggregate level and detailed insight into their distribution. Carotenoids in orange-fleshed sweet potato (OFSP), carrot, and mango were visualized by coherent anti-Stokes Raman scattering (CARS) microscopy and, in the case of OFSP, related to the plant-matrix morphology by simultaneous second-harmonic generation (SHG) microscopy of starch granules. Sizes, shapes, densities, and location of different types of carotenoid bodies were quantified. While OFSP and carrot showed heterogeneous rod-shaped bodies with high carotenoid densities indicated by higher CARS signals, the carotenoid-filled lipid droplets in mango appeared as homogeneous low-density aggregates of rounded shape. In addition, beta-carotene densities and morphologies in OFSP were studied after thermal processing, showing that the bodies remain intact despite significant changes of the surrounding starch granules.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Nyckelord
- coherent anti-Stokes Raman scattering
- carotenoids
- microscopy
- plant cells
- second-harmonic generation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas