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GLYCOLATE OXIDASE3,...
GLYCOLATE OXIDASE3, a Glycolate Oxidase Homolog of Yeast l-Lactate Cytochrome c Oxidoreductase, Supports l-Lactate Oxidation in Roots of Arabidopsis
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- Engqvist, Martin, 1983 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Schmitz, J. (författare)
- Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf
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- Gertzmann, A. (författare)
- Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf
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- Florian, A. (författare)
- Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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- Jaspert, N. (författare)
- Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf
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- Arif, M. (författare)
- Abdul Wali Khan University Mardan,Universität Potsdam,University of Potsdam
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- Balazadeh, S. (författare)
- Universität Potsdam,University of Potsdam
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- Mueller-Roeber, B. (författare)
- Universität Potsdam,University of Potsdam
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- Fernie, A. R. (författare)
- Max Planck Gesellschaft zur Förderung der Wissenschaften e.V. (MPG),Max Planck Society for the Advancement of Science (MPG)
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- Maurino, V. G. (författare)
- Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf
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(creator_code:org_t)
- 2015-08-05
- 2015
- Engelska.
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 1532-2548 .- 0032-0889. ; 169:2, s. 1042-1061
- Relaterad länk:
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http://dx.doi.org/10...
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http://www.plantphys...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- In roots of Arabidopsis (Arabidopsis thaliana), L-lactate is generated by the reduction of pyruvate via L-lactate dehydrogenase, but this enzyme does not efficiently catalyze the reverse reaction. Here, we identify the Arabidopsis glycolate oxidase (GOX) paralogs GOX1, GOX2, and GOX3 as putative L-lactate-metabolizing enzymes based on their homology to CYB2, the L-lactate cytochrome c oxidoreductase from the yeast Saccharomyces cerevisiae. We found that GOX3 uses L-lactate with a similar efficiency to glycolate; in contrast, the photorespiratory isoforms GOX1 and GOX2, which share similar enzymatic properties, use glycolate with much higher efficiencies than L-lactate. The key factor making GOX3 more efficient with L-lactate than GOX1 and GOX2 is a 5- to 10-fold lower Km for the substrate. Consequently, only GOX3 can efficiently metabolize L-lactate at low intracellular concentrations. Isotope tracer experiments as well as substrate toxicity tests using GOX3 loss-offunction and overexpressor plants indicate that L-lactate is metabolized in vivo by GOX3. Moreover, GOX3 rescues the lethal growth phenotype of a yeast strain lacking CYB2, which cannot grow on L-lactate as a sole carbon source. GOX3 is predominantly present in roots and mature to aging leaves but is largely absent from young photosynthetic leaves, indicating that it plays a role predominantly in heterotrophic rather than autotrophic tissues, at least under standard growth conditions. In roots of plants grown under normoxic conditions, loss of function of GOX3 induces metabolic rearrangements that mirror wild-type responses under hypoxia. Thus, we identified GOX3 as the enzyme that metabolizes L-lactate to pyruvate in vivo and hypothesize that it may ensure the sustainment of low levels of L-lactate after its formation under normoxia.
Ämnesord
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Engqvist, Martin ...
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Schmitz, J.
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Gertzmann, A.
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Florian, A.
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Jaspert, N.
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Arif, M.
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visa fler...
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Balazadeh, S.
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Mueller-Roeber, ...
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Fernie, A. R.
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Maurino, V. G.
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Botanik
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Plant Physiology
- Av lärosätet
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Chalmers tekniska högskola