Sökning: onr:"swepub:oai:research.chalmers.se:6debead7-d41a-4b6d-a4b9-6e35d1d7529c" >
Characterization an...
Characterization and kinetic analysis of a thermostable GH3 β-glucosidase from Penicillum brasilianum
-
- Krogh, K.B.R.M. (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Novozymes A/S
-
- Harris, P.V. (författare)
- Novozymes, Inc.
-
Olsen, Carsten L. (författare)
-
visa fler...
-
Salomon Johansen, Katja, 1969 (författare)
-
- Hojer-Pedersen, Jesper (författare)
- Danmarks Tekniske Universitet,Technical University of Denmark,Novozymes A/S
-
- Börjesson, Johan (författare)
- Novozymes A/S,Lunds universitet,Lund University
-
- Olsson, Lisbeth, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Danmarks Tekniske Universitet,Technical University of Denmark
-
visa färre...
-
(creator_code:org_t)
- 2009-09-16
- 2010
- Engelska.
-
Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media LLC. - 1432-0614 .- 0175-7598. ; 86:86, s. 143-154
- Relaterad länk:
-
https://research.cha...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- A GH3 β-glucosidase (BGL) from Penicillum brasilianum was purified to homogeneity after cultivation on a cellulose and xylan rich medium. The BGL was identified in a genomic library, and it was successfully expressed in Aspergillus oryzae. The BGL had excellent stability at elevated temperatures with no loss in activity after 24 h of incubation at 60°C at pH 4-6, and the BGL was shown to have significantly higher stability at these conditions in comparison to Novozym 188 and to other fungal GH3 BGLs reported in the literature. The BGL had significant lower afinity for cellobiose compared with the artificial substrate para-nitrophenyl-β-D-glucopyranoside (pNP-Glc)and further, pronounced substrate inhibition using p-NP-Glc. Kinetic studies demonstrated the high importance of using cellbiose as substrate and glucose as inhibitor to describe the inhibition kinetics of BGL taking place during cellulose hydrolysis. A novel assay was developed to characterize this glucose inhibition on cellobiose hydrolosis. The assay uses labelled glucose-13C6 as inhibitor and subsequent mass spectrometry analysis to quantify the hydrolysis rates
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- Glucose inhibition
- Kinetics
- Purification
- Cellulose hydrolosis
- Fungal expression
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas