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Pathway for the bio...
Pathway for the biosynthesis of the pigment chrysogine by Penicillium chrysogenum
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- Viggiano, Annarita (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Salo, O. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Ali, Hazrat (författare)
- National Institute for Biotechnology and Genetic Engineering Pakistan,Rijksuniversiteit Groningen,University of Groningen
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- Szymanski, Wiktor (författare)
- Rijksuniversiteit Groningen,University of Groningen
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- Lankhorst, P. P. (författare)
- DSM Biotechnology Centre
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- Nygård, Yvonne, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Rijksuniversiteit Groningen,University of Groningen
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- Bovenberg, R. A. L. (författare)
- Rijksuniversiteit Groningen,University of Groningen,DSM Biotechnology Centre
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- Driessen, Arnold J.M. (författare)
- Rijksuniversiteit Groningen,University of Groningen
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - 1098-5336 .- 0099-2240. ; 84:4
- Relaterad länk:
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- Chrysogine is a yellow pigment produced by Penicillium chrysogenum and other filamentous fungi. Although the pigment was first isolated in 1973, its biosynthetic pathway has so far not been resolved. Here, we show that deletion of the highly expressed nonribosomal peptide synthetase (NRPS) gene Pc21g12630 (chyA) resulted in a decrease in the production of chrysogine and 13 related compounds in the culture broth of P. chrysogenum. Each of the genes of the chyAcontaining gene cluster was individually deleted, and corresponding mutants were examined by metabolic profiling in order to elucidate their function. The data suggest that the NRPS ChyA mediates the condensation of anthranilic acid and alanine into the intermediate 2-(2-aminopropanamido)benzoic acid, which was verified by feeding experiments of a ΔchyA strain with the chemically synthesized product. The remainder of the pathway is highly branched, yielding at least 13 chrysogine-related compounds.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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