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Phosphorylation of ...
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
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- Kobir, A. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Poncet, S. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Bidnenko, V. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Delumeau, O. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Jers, C. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Zouhir, S. (författare)
- Centre national de la recherche scientifique (CNRS)
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- Grenha, R. (författare)
- Centre national de la recherche scientifique (CNRS)
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- Nessler, S. (författare)
- Centre national de la recherche scientifique (CNRS),Université Paris-Sud XI,University of Paris-Sud XI
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- Noirot, P. (författare)
- Microbiologie de l'Alimentation au Service de la Sante Humaine
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- Mijakovic, Ivan, 1975 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2014-04-29
- 2014
- Engelska.
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Ingår i: Molecular Microbiology. - : Wiley. - 1365-2958 .- 0950-382X. ; 92:5, s. 1129-1141
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Abstract
Ämnesord
Stäng
- AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biologisk systematik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biological Systematics (hsv//eng)
Nyckelord
- RAUCH MA
- 1997
- 1995
- V179
- MOLECULAR MICROBIOLOGY
- V177
- TRANSITION-STATE REGULATOR
- DOMAIN
- V246
- APRE EXPRESSION
- EMBO JOURNAL
- eilly M
- P522
- RAUCH MA
- TRANSCRIPTION
- P1203
- P1615
- V8
- TYROSINE
- PROMOTER
- RAUCH MA
- JOURNAL OF BACTERIOLOGY
- MOLECULAR & GENERAL GENETICS
- JOURNAL OF BACTERIOLOGY
- RAUCH MA
- V3
- 1995
- 1989
- SPORULATION
- SPECIFICITY
- P756
- P6999
- 1989
- MYCOBACTERIUM-TUBERCULOSIS
- PROTEIN-KINASES
Publikations- och innehållstyp
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Till lärosätets databas
- Av författaren/redakt...
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Kobir, A.
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Poncet, S.
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Bidnenko, V.
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Delumeau, O.
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Jers, C.
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Zouhir, S.
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visa fler...
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Grenha, R.
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Nessler, S.
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Noirot, P.
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Mijakovic, Ivan, ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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Molecular Microb ...
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Chalmers tekniska högskola