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Connection between ...
Connection between protein-tyrosine kinase inhibition and coping with oxidative stress in Bacillus subtilis
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- Shi, Lei, 1981 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Derouiche, Abderahmane, 1980 (författare)
- King Abdullah University of Science and Technology (KAUST),Chalmers tekniska högskola,Chalmers University of Technology
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- Pandit, Santosh, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Alazmi, Meshari (författare)
- University of Hail,King Abdullah University of Science and Technology (KAUST)
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- Ventroux, M. (författare)
- Université Paris-Saclay,University Paris-Saclay
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- Køhler, Julie Bonne (författare)
- Novo Nordisk Fonden,Novo Nordisk Foundation
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- Noirot-Gros, M. F. (författare)
- Université Paris-Saclay,University Paris-Saclay
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- Gao, Xin (författare)
- King Abdullah University of Science and Technology (KAUST)
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- Mijakovic, Ivan, 1975 (författare)
- Novo Nordisk Fonden,Novo Nordisk Foundation,Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - 0027-8424 .- 1091-6490. ; 121:25
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In bacteria, attenuation of protein-tyrosine phosphorylation occurs during oxidative stress. The main described mechanism behind this effect is the H2O2-triggered conversion of bacterial phospho-tyrosines to protein-bound 3,4-dihydroxyphenylalanine. This disrupts the bacterial tyrosine phosphorylation-based signaling network, which alters the bacterial polysaccharide biosynthesis. Herein, we report an alternative mechanism, in which oxidative stress leads to a direct inhibition of bacterial protein-tyrosine kinases (BY-kinases). We show that DefA, a minor peptide deformylase, inhibits the activity of BY-kinase PtkA when Bacillus subtilis is exposed to oxidative stress. High levels of PtkA activity are known to destabilize B. subtilis pellicle formation, which leads to higher sensitivity to oxidative stress. Interaction with DefA inhibits both PtkA autophosphorylation and phosphorylation of its substrate Ugd, which is involved in exopolysaccharide formation. Inactivation of defA drastically reduces the capacity of B. subtilis to cope with oxidative stress, but it does not affect the major oxidative stress regulons PerR, OhrR, and Spx, indicating that PtkA inhibition is the main pathway for DefA involvement in this stress response. Structural analysis identified DefA residues Asn95, Tyr150, and Glu152 as essential for interaction with PtkA. Inhibition of PtkA depends also on the presence of a C-terminal α-helix of DefA, which resembles PtkA-interacting motifs from known PtkA activators, TkmA, SalA, and MinD. Loss of either the key interacting residues or the inhibitory helix of DefA abolishes inhibition of PtkA in vitro and impairs postoxidative stress recovery in vivo, confirming the involvement of these structural features in the proposed mechanism.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- bacterial protein-tyrosine kinases
- oxidative stress
- protein-tyrosine phosphorylation
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Till lärosätets databas
- Av författaren/redakt...
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Shi, Lei, 1981
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Derouiche, Abder ...
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Pandit, Santosh, ...
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Alazmi, Meshari
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Ventroux, M.
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Køhler, Julie Bo ...
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visa fler...
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Noirot-Gros, M. ...
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Gao, Xin
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Mijakovic, Ivan, ...
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visa färre...
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Chalmers tekniska högskola