Sökning: onr:"swepub:oai:research.chalmers.se:d118f284-5244-4a13-83be-4a7640b60648" >
Genomic and transcr...
Genomic and transcriptomic analysis of the thermophilic lignocellulose-degrading fungus Thielavia terrestris LPH172
-
- Tõlgo, Monika, 1994 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Hüttner, Silvia, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Rugbjerg, Peter, 1988 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
visa fler...
-
Thuy, N. T. (författare)
-
Thanh, V. N. (författare)
-
- Larsbrink, Johan, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
- Olsson, Lisbeth, 1963 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
-
visa färre...
-
(creator_code:org_t)
- 2021-06-03
- 2021
- Engelska.
-
Ingår i: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834 .- 1754-6834. ; 14:1
- Relaterad länk:
-
https://research.cha... (primary) (free)
-
visa fler...
-
https://biotechnolog...
-
https://research.cha...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification and make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active enzymes (CAZymes) for biomass degradation. The newly isolated LPH172 strain of the thermophilic Ascomycete Thielavia terrestris has been shown to possess high xylanase and cellulase activities and tolerate low pH and high temperatures. Here, we aimed to illuminate the lignocellulose-degrading machinery and novel carbohydrate-active enzymes in LPH172 in detail. Results: We sequenced and analyzed the 36.6-Mb genome and transcriptome of LPH172 during growth on glucose, cellulose, rice straw, and beechwood xylan. 10,128 predicted genes were found in total, which included 411 CAZy domains. Compared to other fungi, auxiliary activity (AA) domains were particularly enriched. A higher GC content was found in coding sequences compared to the overall genome, as well as a high GC3 content, which is hypothesized to contribute to thermophilicity. Primarily auxiliary activity (AA) family 9 lytic polysaccharide monooxygenase (LPMO) and glycoside hydrolase (GH) family 7 glucanase encoding genes were upregulated when LPH172 was cultivated on cellulosic substrates. Conventional hemicellulose encoding genes (GH10, GH11 and various CEs), as well as AA9 LPMOs, were upregulated when LPH172 was cultivated on xylan. The observed co-expression and co-upregulation of genes encoding AA9 LPMOs, other AA CAZymes, and (hemi)cellulases point to a complex and nuanced degradation strategy. Conclusions: Our analysis of the genome and transcriptome of T. terrestris LPH172 elucidates the enzyme arsenal that the fungus uses to degrade lignocellulosic substrates. The study provides the basis for future characterization of potential new enzymes for industrial biomass saccharification.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- Thermostable enzymes
- Carbohydrate active enzymes
- LPMO
- Xylan
- Cellulose
- Transcriptome
- Biomass degradation
- Filamentous fungi
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Tõlgo, Monika, 1 ...
-
Hüttner, Silvia, ...
-
Rugbjerg, Peter, ...
-
Thuy, N. T.
-
Thanh, V. N.
-
Larsbrink, Johan ...
-
visa fler...
-
Olsson, Lisbeth, ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Mikrobiologi
-
- TEKNIK OCH TEKNOLOGIER
-
TEKNIK OCH TEKNO ...
-
och Industriell biot ...
-
och Biokatalys och e ...
- Artiklar i publikationen
-
Biotechnology fo ...
- Av lärosätet
-
Chalmers tekniska högskola