Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.

• Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the detection of native and exogenous compounds in biological samples on a cellular scale. Through the development of novel ion beams the amount of molecular signal available from the sample surface has been increased. Through the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be used to monitor molecular signals as a function of depth as the sample is eroded thus proving the ability to generate 3D molecular images. Here we describe how this new capability has led to the development of novel instrumentation for 3D molecular imaging while also highlighting the importance of sample preparation and discuss the challenges that still need to be overcome to maximise the impact of the technique.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

Cell Membrane

Spectrometry

analysis

Animals

methods

instrumentation

Xenopus laevis

Mass

Software

Humans

analysis

chemistry

Phospholipids

Three-Dimensional

Ions

methods

instrumentation

HeLa Cells

Amino Acids

instrumentation

Molecular Imaging

Secondary Ion

Imaging

Principal Component Analysis

methods

chemistry

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