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Time-of-flight SIMS...
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Armitage, Emily G
(författare)
Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer
- Artikel/kapitelEngelska2013
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Nummerbeteckningar
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LIBRIS-ID:oai:research.chalmers.se:dea6d3d2-6b92-48ef-a90a-0a201b5760be
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https://research.chalmers.se/publication/178868URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Kotze, Helen L
(författare)
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Fletcher, John,1978
(författare)
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Williams, Kaye J
(författare)
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Lockyer, Nicholas P.
(författare)
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Vickerman, John C
(författare)
Sammanhörande titlar
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Ingår i:Surface and Interface Analysis45:1, s. 282-2851096-99180142-2421
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