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Novel bilayer bacte...
Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo
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- Martinez Avila, Hector, 1985 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Feldmann, Eva-Maria (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Pleumeekers, M.M. (författare)
- Erasmus Universiteit Rotterdam,Erasmus University Rotterdam
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visa fler...
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- Nimeskern, L. (författare)
- Eidgenössische Technische Hochschule Zürich (ETH),Swiss Federal Institute of Technology in Zürich (ETH)
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- Kuo, W. (författare)
- Eidgenössische Technische Hochschule Zürich (ETH),Swiss Federal Institute of Technology in Zürich (ETH)
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De Jong, W.C. (författare)
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- Schwarz, S. (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Müller, R. (författare)
- Eidgenössische Technische Hochschule Zürich (ETH),Swiss Federal Institute of Technology in Zürich (ETH)
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Hendriks, J.A.A. (författare)
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- Rotter, N. (författare)
- Universitätsklinikum Ulm,University Hospital of Ulm
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- Van Osch, G.J.V.M. (författare)
- Erasmus Universiteit Rotterdam,Erasmus University Rotterdam
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- Stok, K. S. (författare)
- Eidgenössische Technische Hochschule Zürich (ETH),Swiss Federal Institute of Technology in Zürich (ETH)
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- Gatenholm, Paul, 1956 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Biomaterials. - : Elsevier BV. - 0142-9612 .- 1878-5905. ; 44, s. 122-133
- Relaterad länk:
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http://dx.doi.org/10...
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https://www.research...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Tissue engineering provides a promising alternative therapy to the complex surgical reconstruction of auricular cartilage by using ear-shaped autologous costal cartilage. Bacterial nanocellulose (BNC) is proposed as a promising scaffold material for auricular cartilage reconstruction, as it exhibits excellent biocompatibility and secures tissue integration. Thus, this study evaluates a novel bilayer BNC scaffold for auricular cartilage tissue engineering. Bilayer BNC scaffolds, composed of a dense nanocellulose layer joined with a macroporous composite layer of nanocellulose and alginate, were seeded with human nasoseptal chondrocytes (NC) and cultured invitro for up to 6 weeks. To scale up for clinical translation, bilayer BNC scaffolds were seeded with a low number of freshly isolated (uncultured) human NCs combined with freshly isolated human mononuclear cells (MNC) from bone marrow in alginate and subcutaneously implanted in nude mice for 8 weeks. 3D morphometric analysis showed that bilayer BNC scaffolds have a porosity of 75% and mean pore size of 50±25μm. Furthermore, endotoxin analysis and invitro cytotoxicity testing revealed that the produced bilayer BNC scaffolds were non-pyrogenic (0.15±0.09EU/ml) and non-cytotoxic (cell viability: 97.8±4.7%). This study demonstrates that bilayer BNC scaffolds offer a good mechanical stability and maintain a structural integrity while providing a porous architecture that supports cell ingrowth. Moreover, bilayer BNC scaffolds provide a suitable environment for culture-expanded NCs as well as a combination of freshly isolated NCs and MNCs to form cartilage invitro and invivo as demonstrated by immunohistochemistry, biochemical and biomechanical analyses.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biomaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bio Materials (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
Nyckelord
- Bacterial cellulose
- Tissue engineering
- Ear cartilage
- Neo-cartilage
- Mononuclear cells
- Nasoseptal chondrocytes
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Martinez Avila, ...
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Feldmann, Eva-Ma ...
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Pleumeekers, M.M ...
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Nimeskern, L.
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Kuo, W.
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De Jong, W.C.
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visa fler...
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Schwarz, S.
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Müller, R.
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Hendriks, J.A.A.
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Rotter, N.
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Van Osch, G.J.V. ...
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Stok, K. S.
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Gatenholm, Paul, ...
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Industriell biot ...
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och Biomaterial
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomaterialveten ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Medicinteknik
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och Medicinsk materi ...
- Artiklar i publikationen
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Biomaterials
- Av lärosätet
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Chalmers tekniska högskola