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The fine structure ...
The fine structure of cassava starch amylopectin Part 1: Organization of clusters
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- Bertoft, Eric (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science
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(creator_code:org_t)
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- Elsevier BV, 2010
- 2010
- Engelska.
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 47, s. 317-324
- Relaterad länk:
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The enzyme alpha-amylase from Bacillus subtilis was applied to partly hydrolyze purified cassava amylopectin into groups of clusters, which were called domains. The domains were further size-fractionated by methanol precipitation and then subjected to a second stage of alpha-amylolysis until the rate of hydrolysis was slow in order to release the single clusters. All domain and cluster fractions were hydrolyzed with beta-amylase into beta-limit dextrins. The size distribution and chain composition of the beta-limit dextrins were analyzed by gel-permeation chromatography and high-performance anion-exchange chromatography with pulsed amperometric detection, respectively. The sizes of the clusters in the form of beta-limit dextrins were uniform with an average degree of polymerization of 67-78. The distribution profiles of B-chains were similar in all cluster fractions, which suggested that the internal structure of the cassava amylopectin clusters was homogenous. Long B-chains were involved in the interconnection of clusters in the domain fractions. These were cleaved and a new group of chains of intermediate length was produced by the alpha-amylase together with short chains. In the isolated clusters, however, some chains corresponding to long B-chains still remained, which is not predicted by the traditional cluster model of the amylopectin structure. Instead, the alternative two-directional backbone model could explain the mode of interconnection between the clusters. (C) 2010 Elsevier B.V. All rights reserved.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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