Analysis of the hybrid genomes of two field isolates of the soil-borne fungal species Verticillium longisporum

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: onr:"swepub:oai:slubar.slu.se:93436" > Analysis of the hyb...

1 av 1
Föregående post
Nästa post
Till träfflistan

Fogelqvist, JohanSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology (författare)

Analysis of the hybrid genomes of two field isolates of the soil-borne fungal species Verticillium longisporum

Artikel/kapitelEngelska2018

Förlag, utgivningsår, omfång ...

2018-01-03
Springer Science and Business Media LLC,2018
BioMed Central,2024

Nummerbeteckningar

LIBRIS-ID:oai:slubar.slu.se:93436
https://res.slu.se/id/publ/93436URI
https://doi.org/10.1186/s12864-017-4407-xDOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Background: Brassica plant species are attacked by a number of pathogens; among them, the ones with a soilborne lifestyle have become increasingly important. Verticillium stem stripe caused by Verticillium longisporum is one example. This fungal species is thought to be of a hybrid origin, having a genome composed of combinations of lineages denominated A and D. In this study we report the draft genomes of 2 V. longisporum field isolates sequenced using the Illumina technology. Genomic characterization and lineage composition, followed by selected gene analysis to facilitate the comprehension of its genomic features and potential effector categories were performed.Results: The draft genomes of 2 Verticillium longisporum single spore isolates (VL1 and VL2) have an estimated ungapped size of about 70 Mb. The total number of protein encoding genes identified in VL1 was 20,793, whereas 21,072 gene models were predicted in VL2. The predicted genome size, gene contents, including the gene families coding for carbohydrate active enzymes were almost double the numbers found in V. dahliae and V. albo-atrum. Single nucleotide polymorphisms (SNPs) were frequently distributed in the two genomes but the distribution of heterozygosity and depth was not independent. Further analysis of potential parental lineages suggests that the V. longisporum genome is composed of two parts, A1 and D1, where A1 is more ancient than the parental lineage genome D1, the latter being more closer related to V. dahliae. Presence of the mating-type genes MAT1-1-1 and MAT1-2-1 in the V. longisporum genomes were confirmed. However, the MAT genes in V. dahliae, V. albo-atrum and V. longisporum have experienced extensive nucleotide changes at least partly explaining the present asexual nature of these fungal species.Conclusions: The established draft genome of V. longisporum is comparatively large compared to other studied ascomycete fungi. Consequently, high numbers of genes were predicted in the two V. longisporum genomes, among them many secreted proteins and carbohydrate active enzyme (CAZy) encoding genes. The genome is composed of two parts, where one lineage is more ancient than the part being more closely related to V. dahliae. Dissimilar mating-type sequences were identified indicating possible ancient hybridization events.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

Tzelepis, GeorgiosSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)46953 (författare)
Bejai, SaroshSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)47284 (författare)
Roos, JonasSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)50244 (författare)
Schwelm, ArneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)49819 (författare)
Dixelius, ChristinaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology(Swepub:slu)48572 (författare)
Sveriges lantbruksuniversitetInstitutionen för växtbiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

Sammanhörande titlar

Ingår i:BMC Genomics: Springer Science and Business Media LLC191471-2164

Internetlänk

Hitta via bibliotek

BMC Genomics (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

1 av 1
Föregående post
Nästa post
Till träfflistan

Hitta mer i SwePub

Av författaren/redakt...: Fogelqvist, Joha ...; Tzelepis, Georgi ...; Bejai, Sarosh; Roos, Jonas; Schwelm, Arne; Dixelius, Christ ...

Om ämnet

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Bioteknologi med ...; och Genetik och förä ...

Artiklar i publikationen: BMC Genomics

Av lärosätet: Sveriges Lantbruksuniversitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se