| 1. |
- Morrell, Jane, et al.
(author)
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Practical applications of sperm selection techniques for improving reproductive efficiency
- 2016
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In: Animal Reproduction. - 1806-9614 .- 1984-3143. ; 13, s. 340-345
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Conference paper (peer-reviewed)abstract
- Several selection techniques are available for processing spermatozoa. Apart from sperm washing to remove seminal plasma, only "swim-up" and colloid centrifugation have been used to any extent to prepare spermatozoa for in vitro fertilization, and only colloid centrifugation has been used to prepare sperm samples for artificial insemination. Single-layer centrifugation (SLC) through a species-specific colloid has been shown to be effective in selecting spermatozoa with good motility, normal morphology and intact chromatin in a range of species. This method is less time-consuming than swim-up, and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The applications of SLC are as follows: to improve sperm quality in insemination doses or in samples for in vitro fertilization, to increase the shelf life of normal sperm doses, to remove pathogens ( viruses, bacteria), to improve cryosurvival by removing dead and dying spermatozoa before freezing or after thawing, to select spermatozoa for intracytoplasmic sperm injection, and to aid conservation breeding.
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| 2. |
- Morrell, Jane, et al.
(author)
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Practical implications of sperm selection techniques for improving reproduction
- 2017
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In: Animal Reproduction. - 1806-9614 .- 1984-3143. ; 14, s. 572-580
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Journal article (peer-reviewed)abstract
- Sperm selection techniques are needed to separate spermatozoa from seminal plasma and extender for in vitro fertilization (IVF) and to improve sperm quality for a range of assisted reproduction techniques. Apart from sperm washing, which removes some but not all of the seminal plasma, the selection techniques that are currently used are mainly swim-up and colloid centrifugation; filtration through Sephadex columns or glass wool is seldom used in the field. Although swim-up can be used to prepare sperm samples for IVF, the low recovery rate and lack of selection for sperm quality other than motility make this technique ineffective for routine use. Colloid centrifugation is used to prepare semen for all types of assisted reproduction. The method has been scaled-up for voluminous ejaculates e.g. from stallion and boar, and scaled-down to accommodate small volumes of thawed semen (e.g. from bull). Sperm quality and fertility are improved, as shown in laboratory assays and in various fertility trials. Some normal spermatozoa are lost during the selection process but overall the advantages of improved longevity and fertility in the selected spermatozoa outweigh the disadvantages. Since spermatozoa are separated from bacteria in the ejaculate, it may be possible to reduce antibiotic usage in semen extenders. New applications of colloid centrifugation include extracting camelid spermatozoa from viscous seminal plasma, selecting spermatozoa with condensed chromatin (i.e. with fewer free thiols), and using the number of spermatozoa passing through the colloid as a diagnostic tool to indicate male fertility.
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| 3. |
- Humblot, Patrice
(author)
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From clinics to (cow)mics: a reproductive journey
- 2018
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In: Animal Reproduction. - 1806-9614 .- 1984-3143. ; 15, s. 278-291
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Journal article (peer-reviewed)abstract
- This manuscript describes the different topics I have been involved in the fields of reproductive physiology and embryo biotechnologies with attempts to address practical issues raised mainly by the breeding industry. The journey started with phenotyping work in the field of reproductive physio-pathology. Other issues were related to the optimization of reproductive biotechnologies to favorize genetic selection. The implementation of genomic selection raised opportunities to develop the use embryo biotechnologies and showed the interest of combining them in the case of embryo genotyping. There is still a need to refine phenotyping for reproductive traits especially for the identification of markers of uterine dysfunction. It is believed that new knowledge generated by combining different molecular approaches will be the source of applications that may benefit AI practice and embryo technologies.
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| 4. |
- Morrell, Jane
(author)
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Comparison of sperm selection techniques in donkeys: motile subpopulations from a practical point of view
- 2019
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In: Animal Reproduction. - 1806-9614 .- 1984-3143. ; 16, s. 282-289
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Journal article (peer-reviewed)abstract
- The aim of this study was to compare the post-thaw distribution of motile sperm subpopulations, following simple or colloid centrifugation. A new analysis was used to evaluate the available number of sperm from each subpopulation after each centrifugation protocol. Frozen/thawed semen samples were divided into the following after-thawing treatments: uncentrifuged control (UDC), sperm washing (SW) and two colloid centrifugation procedures (Equipure, SLC-E, and Androcoll, SLC-A). Percentage of total and progressive motility (TM and PM), as well as sperm motility kinematics, distribution of motile sperm subpopulations, and recovery rates, were statistically compared among treatments. The SLC treatments showed higher (P < 0.001) TM and PM than UDC and SW. Following each SLC procedure, different percentages of the subpopulation with the most vigorous and progressive sperm (sP4) were obtained. SLC-A recovered a larger number of sperm belonging to sP4 than SLC-E, but not significantly higher than SW. From a practical point of view, sperm washing, the standard centrifugation procedure for equine semen processing, recovered the same amount of fast and progressive sperm as colloid centrifugation, apparently the best treatment according to traditional analysis. In conclusion, samples processed by SLC have higher motility percentages than SW and UDC but, after combining the available number of sperm, SLC and SW techniques are equally efficient in recovering sperm from the most vigorous, fast and progressive motile subpopulation (sP4).
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| 5. |
- Parrilla, Inmaculada, et al.
(author)
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Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine
- 2020
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In: Animal Reproduction. - : BRAZILIAN COLL ANIMAL REPRODUCTION. - 1806-9614 .- 1984-3143. ; 17:3
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Journal article (peer-reviewed)abstract
- Seminal plasma (SP) supports not only sperm function but also the ability of spermatozoa to withstand biotechnological procedures as artificial insemination, freezing or sex sorting. Moreover, evidence has been provided that SP contains identifiable molecules which can act as fertility biomarkers, and even improve the output of assisted reproductive technologies by acting as modulators of endometrial and embryonic changes of gene expression, thus affecting embryo development and fertility beyond the sperm horizon. In this overview, we discuss current knowledge of the composition of SP, mainly proteins and cytokines, and their influence on semen basic procedures, such as liquid storage or cryopreservation. The role of SP as modulator of endometrial and embryonic molecular changes that lead to successful pregnancy will also be discussed.
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