| 1. |
- Beech, Jason P., et al.
(author)
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Capillary driven separation on patterned surfaces
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 785-787
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Conference paper (peer-reviewed)abstract
- Deterministic lateral displacement (DLD) is a powerful bimodal separation scheme [1] based on fluid flow through regular obstacle arrays that in its basic embodiment sends suspended particles in two different directions as a function of size. We show that without the need to seal devices and without the need for fluidic connections or pumps, particle separation can be achieved by the passive flow of a sample over a patterned surface. Risk of clogging is minimized by the movement of large particles above the obstacle array. Suitable application areas include blood fractionation and analysis of drinking water. 0
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| 2. |
- Beech, Jason P., et al.
(author)
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Gravitationally driven deterministic lateral displacement devices
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 779-781
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Conference paper (peer-reviewed)abstract
- Deterministic lateral displacement (DLD) is a powerful bimodal separation scheme [1] based on regular obstacle arrays that in its basic embodiment sends particles in two different directions as a function of size. We add functionality to the technique by including gravitational forces, as a perturbation to particles transported by fluid flow, and as a way of transporting the particles through a stationary fluid.
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| 3. |
- Beech, Jason P., et al.
(author)
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Shape-based particle sorting - A new paradigm in microfluidics
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 800-802
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Conference paper (peer-reviewed)abstract
- Conventional fractionation techniques fail to fully benefit from the variety in morphology and shape that is found among biological particles. Although light scattering in conventional FACS gives some information on the size and morphology of a particle, it is generally not capable of giving a definite number on specified dimensions of a small object. We demonstrate an approach where we select which dimension of a particular object is used to determine its trajectory through an obstacle course and thereby sort not merely with respect to hydrodynamic radius but rather with respect to e.g. thickness, length or width.
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| 4. |
- Gantelius, Jesper, et al.
(author)
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Improved sensitivity on an allergen lateral flow microarray by means of dendritic amplification
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 1136-1137
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Conference paper (peer-reviewed)abstract
- Recently, paper-based substrates have been proposed as an alternative to commonly used activated glass slides for microarray patterning[1], used in conjunction with capillary driven lateral flow of sample and detection reagents through the membrane. While fluorescent detection reagents may be employed to achieve high sensitivity, gold nanoparticles can also be used to allow readout by means of common table top scanners or digital cameras. Here, we demonstrate first results from employing a dendritic, or layer-by-layer, amplification approach for a high-density lateral flow allergen protein microarray, indicating that substantially increased sensitivity can be achieved with very modest increase of assay handling and time requirements.
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| 5. |
- Gylfason, Kristinn B., 1978-, et al.
(author)
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A packaged optical slot-waveguide ring resonator sensor array for multiplex assays in labs-on-chip
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 2004-2006
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Conference paper (peer-reviewed)abstract
- We present the design, fabrication, and characterization of a packaged array of individually addressable slot-waveguide ring resonator sensors in a compact cartridge for sensitive, label-free, multiplex assays. The novel use of a dual surfaceenergy adhesive film enables simple generic packaging method for multiple sensors in a single cartridge. The use of optical slot-waveguides, and drift compensation by on-chip light splitting to reference sensors, gives the best refractive-index limit of detection reported for planar ring resonator sensors.
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| 6. |
- Järås, Kerstin, et al.
(author)
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PSA quantification of 80 plasma samples from the clinical routine using antibody microarrays
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 457-459
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Conference paper (peer-reviewed)abstract
- Antibody microarrays are becoming increasingly established in clinical studies. However, the arrays are seldom used in a quantitative approach but rather for detecting up or down regulated proteins. In this study we describe a microarray procedure being standardized by placing the in-house developed porous silicon surfaces into a commercially available 96 well microtiter plate for analyzing the PSA concentrationsof 80 EDTA-plasma samples obtained from patients undergoing clinical PSAtesting.
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| 7. |
- Lenshof, Andreas, et al.
(author)
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Acoustic whole blood plasmapheresis chip for PSA microarray diagnostics
- 2009
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In: Proceedings of Conference, MicroTAS 2009 : The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806421 ; , s. 1330-1332
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Conference paper (peer-reviewed)abstract
- An acoustic device for generating high quality blood plasma for PSA microarray diagnostics is presented.
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| 8. |
- Lindström, Sara, et al.
(author)
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A microwell chip for parallel culture and analysis of stem cells
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 1309-1311
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Conference paper (peer-reviewed)abstract
- With recent findings on the role of reprogramming factors on stem cells, in vitro screening assays for studying (de)-differentiation is of great interest. We developed a miniaturized stem cell screening chip that is easily accessible and provides means of rapidly studying thousands of individual stem/progenitor cell samples, using low reagent volumes. Results presented here include weeklong culturing and differentiation assays of mouse embryonic stem cells, mouse adult neural stem cells, and human embryonic stem cells. The possibility to maintain the cells as stem/progenitor cells over time was shown, as was isolation and clonal analysis.
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| 9. |
- Ohashi, T., et al.
(author)
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Novel bioassay system for single cells
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 1171-1173
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Conference paper (peer-reviewed)abstract
- In this paper, we propose a novel bioassay system consisting of an active microfluidic device integrated on a microwell plate for high throughput single-cell analysis. Firstly, a CFD (computational fluid dynamics) simulation is performed to determine flow fields in order to assess how fluid flow reaches cells cultured in the wells in the microwell plate. It is then confirmed that the cells are appropriately stained with Calcein AM by fluid flow generated by magnetically driven micropumps. The proposed bioassay system would be a powerful platform for single-cell characterization in a high throughput manner.
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| 10. |
- Russom, Aman, et al.
(author)
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Blood cells separation in spiral microchannels
- 2009
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In: Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806421 ; , s. 1222-1224
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Conference paper (peer-reviewed)abstract
- As major players in our defense system, white blood cells (WBCs) are essential for immunological tests. However, WBCs exist at low concentration in blood samples, typically less than 0.5% of blood cells, and separation or enrichment of leukocytes is therefore a critical initial step in many biological experiments. This paper reports a novel method, based on differential inertial focusing, for separating blood cells in high throughput fashion (volumetric flow rates >2 ml/min). In this method, inherent hydrodynamic forces developed in flow through curved channels force cells to migrate over streamlines and focus laterally in equilibrium positions based on size.
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