| 1. |
- Augustsson, P., et al.
(author)
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Iso-acoustic focusing for size-insensitive cell separation based on acoustic properties
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806483 ; , s. 14-16
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Conference paper (peer-reviewed)abstract
- We report a novel size-insensitive method, iso-acoustic focusing (IAF), that can, for the first time, analyze and separate cells based on their intrinsic acoustic properties. We characterize the IAF system using two different cell lines and apply it to measuring the acoustic impedance of two subgroups of white blood cells (WBC): neutrophils and lymphocytes. We find cell type specific differences in acoustic properties that indicates that this method has potential for cell phenotyping and cell separations.
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| 2. |
- Banerjee, I., et al.
(author)
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Slipdisc : A versatile sample preparation platform
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1256-1258
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Conference paper (peer-reviewed)abstract
- We present "SlipDisc", a versatile sample preparation platform based on slipchip1 technology. The SlipDisc platform uses polycarbonate CDs and laser cut PSA instead of glass and a hand-winded mechanical clock mechanism to precisely manipulate minute amount of liquid. The innovative hand-winded mechanical "clockwork" that enables sample processing from one spot to another with defined precision. As a prof of principle of bioassay, we show HRP enzyme reacting with TMB substrate and a multilayer architecture used in manipulation of magnetic beads through an immiscible oil phase. Our long-term goal is to develop a sample-in-result-out multi-parametric bioanalytical SlipDisc platform specifically designed to need the needs at resource-limited settings for point of care molecular diagnostics.
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| 3. |
- Björk, S. M., et al.
(author)
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Tuning microfluidic cell culture conditions for droplet based screening by metabolite profiling
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1377-1379
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Conference paper (peer-reviewed)abstract
- We investigate the impact of droplet culture conditions on cell metabolic state by determining key metabolite concentrations in S. cerevisiae cultures in different microfluidic droplet culture formats. Control of culture conditions is critical for single cell screening in droplets, as cell metabolic state directly affects production yields in cell factories. Metabolite profiling provides a more nuanced estimate of cell state compared to proliferation studies alone. We show that the choice of droplet incubation format has an impact on cell proliferation and metabolite production. Furthermore, we engineered a new better oxygenated droplet incubation format, with retained droplet stability and size.
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| 4. |
- Christakou, Athanasia, et al.
(author)
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Characterization of natural killer cell immune surveillance against solid liver tumors
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 915-917
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Conference paper (peer-reviewed)abstract
- We demonstrate a method for investigating natural killer (NK) cell aggression against ultrasound-assisted human hepatocellular carcinoma (HCC) HepG2 solid tumors in a multi-well microdevice. We quantify the activity of human primary IL-2 activated NK cells against HepG2 tumors for up to five days and we present the correlation between NK cell numbers versus average tumor volume and final tumor outcome (growth or defeat). We suggest future applications on formation of tumors originated from primary tumors cells and other tumor components as well as primary NK originating from the patient for use in personalized immunotherapy.
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| 5. |
- Evander, Mikael, et al.
(author)
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Non-contact acoustic capture of platelet-derived microparticles from small plasma volumes
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806483 ; , s. 469-471
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Conference paper (peer-reviewed)abstract
- This paper presents the characterization and optimization of acoustic capture of platelet-derived microparticles (PMPs) from human plasma. The technique is compared to a standard preparation protocol using differential centrifugation on biobank samples from patients with ST-elevation myocardial infarction (STEMI). The main advantages of this new method are the capability of rapidly capturing microvesicles from small sample volumes and the potential to extract intact vesicles for downstream processing or handling.
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| 6. |
- Fornell, Anna, et al.
(author)
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Acoustic focusing of microparticles in two-phase systems - Towards cell enrichment or medium exchange in droplets
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1026-1028
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Conference paper (peer-reviewed)abstract
- We present a method to first laterally position microparticles inside droplets by acoustic forces and then split the droplet into three daughter droplets to achieve a 2+fold enrichment of microparticles inside the center droplet. We show that acoustic forces can be applied to both manipulate polystyrene beads (5 μm) and red blood cells inside droplets. The presented technology opens up for development of droplet operations used for medium exchange and particle concentration in droplet-based cell assays.
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| 7. |
- Guo, Maoxiang, et al.
(author)
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LONG-TERM STORAGE OF NANOLITRE AND PICOLITRE LIQUID VOLUMES IN POLYMER MICROFLUIDIC DEVICES
- 2015
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In: the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Micro TAS). - : The Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1386-1388
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Conference paper (peer-reviewed)abstract
- We introduce uncomplicated nanolitre (23 nL) and picolitre (3.5 pL) liquid volume encapsulation in Off-Stoichiometry Thiol-Ene-Epoxy polymer (OSTEmerTM322) wells using spontaneous room- temperature bonding of gold films to thiol and thioether groups present on the surface of the polymer for leak free sealing. First, we show liquid encapsulation within nL, and pL polymer wells by utilizing 100 nm thin Au-film transfer-bonding onto intermediately cured, and micropatterned OSTEmerTM322. This approach yielded 3 magnitude orders smaller liquid volume encapsulation than previously reported. Secondly, we show that encapsulated liquid can be stored for >116 h. Finally, we demonstrate encapsulated liquid release by thermopneumatic bursting. We conclude that OSTEmerTM322 is excellent for metal-film sealant integration in polymer microfluidic devices.
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| 8. |
- Hansson, Jonas, et al.
(author)
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Synthetic microfluidic paper allows controlled receptor positioning and improved readout signal intensity in lateral flow assays
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 284-286
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Conference paper (peer-reviewed)abstract
- Synthetic Microfluidic Paper consists of slanted and interlocked polymer micropillars and can be used as a porous substrate in microfluidics and lateral flow assays. We here demonstrate single step manufacturing of multiple Synthetic Microfluidic Paper densities in the same device, and passive alignment of liquid spots in denser substrate regions, regardless of spotting position, allowing increased control of receptor positioning for lateral flow assays. We further demonstrate that the transparency of Synthetic Microfluidic Paper allows increasing readout signal intensity with increasing substrate thickness, to a value 3 times larger compared to nitrocellulose substrates.
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| 9. |
- Iranmanesh, Ida, et al.
(author)
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Acoustic micro-vortexing of fluids, beads and cells in disposible microfluidic chips
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. - 9780979806483 ; , s. 1005-1007
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Conference paper (peer-reviewed)abstract
- In this paper we demonstrate a multi-functional platform using ultrasound for vortexing of 20-μl volumes of different samples in polymer-based disposable chips. The method enables different vortexing functions such as mixing laminar flows, resuspension of a micro-pellet of magnetic beads as well as cell lysis for DNA extraction. The device consists of an inexpensive low-frequency, high power, horn-shaped langevin transducer which is typically used for cell disruption in larger volumes. By controlling the operating time of this device (from fractions of a second up to a minute) different functions can be achieved. In addition, to avoid the high-power-induced heating, a simple cooling system is used as a chip holder consisting of a PC fan-cooled aluminum heat sink. To demonstrate a sample preparation application, we perform on-chip cell lysis and DNA extraction.
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| 10. |
- Jakobsson, Ola, et al.
(author)
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Thousandfold enrichment of cells using recirculation in an acoustofluidic device
- 2015
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In: MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9780979806483 ; , s. 349-351
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Conference paper (peer-reviewed)abstract
- This paper demonstrates a new acoustofluidic approach to enrich extremely dilute cell suspensions 1000 times and higher, at flow rates of ≥0.5 mL/min and higher, addressing some of the needs in rare cell processing and water quality monitoring.
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