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Träfflista för sökning "L773:9781493926398 OR L773:9781493926381 "

Search: L773:9781493926398 OR L773:9781493926381

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  • Teixeira, Pedro F., et al. (author)
  • A Flowchart to Analyze Protease Activity in Plant Mitochondria
  • 2015
  • In: Plant Mitochondria. - New York, NY : Springer-Verlag New York. - 9781493926398 - 9781493926381 ; 1305, s. 123-30
  • Book chapter (peer-reviewed)abstract
    • Proteases are one of the most abundant classes of enzymes and are involved in a plethora of biological processes in many cellular compartments, including the mitochondria. To understand the role of proteases is essential to determine their substrate repertoire, preferably in an in vivo setting. In this chapter we describe general guidelines to analyze protease activity using several strategies, from in-gel analysis to mass spectrometry mapping of the cleavage site(s) and fluorogenic probes that can easily be used in vivo. To exemplify this flowchart, we used the recently characterized organellar oligopeptidase of Arabidopsis (Arabidopsis thaliana), an enzyme that takes part in degradation of short peptides within mitochondria and chloroplasts.
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Type of publication
book chapter (1)
Type of content
peer-reviewed (1)
Author/Editor
Glaser, Elzbieta (1)
Branca, Rui M. (1)
Teixeira, Pedro F. (1)
Kmiec, Beata (1)
University
Stockholm University (1)
Karolinska Institutet (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Medical and Health Sciences (1)
Year
2015 (1)

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