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Search: WFRF:(Alvang Ronny)

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  • Koeck, Philip J. B., et al. (author)
  • Single particle refinement in electron crystallography : A pilot study
  • 2007
  • In: Journal of Structural Biology. - : Elsevier BV. - 1047-8477 .- 1095-8657. ; 160:3, s. 344-352
  • Journal article (peer-reviewed)abstract
    • Electron crystallography can be used to determine the structures of membrane proteins at near-atomic resolution in some cases. However, most electron crystallography projects remain at a resolution around 10 Å. This might be partly due to lack of flatness of many two-dimensional crystals. We have investigated this problem and suggest single particle processing of locally averaged unit cells to improve the quality and possibly the resolution of three-dimensional maps. Applying this method to the secondary transporter melibiose permease we have calculated a three-dimensional map that is clearer and easier to interpret than the map derived using purely electron-crystallographic methods.
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Type of publication
journal article (1)
Type of content
peer-reviewed (1)
Author/Editor
Hebert, Hans (1)
Koeck, Philip J. B. (1)
Purhonen, P (1)
Alvang, Ronny (1)
Grundberg, Björn (1)
University
Royal Institute of Technology (1)
Södertörn University (1)
Karolinska Institutet (1)
Language
English (1)
Research subject (UKÄ/SCB)
Natural sciences (1)
Year

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