| 1. |
- Alvehus, Malin, 1975-
(author)
-
Obesity-associated inflammation in adipose tissue
- 2012
-
Doctoral thesis (other academic/artistic)abstract
- Background: Excess body fat, particularly in the visceral depot, is linked to increased mortality and morbidity, including the development of diseases such as type 2 diabetes, cardiovascular disease, and cancer. Chronic low-grade inflammation in adipose tissue may be a key mediator of obesity-associated diseases. Importantly, specific pro-inflammatory cytokines have been shown to influence adipose tissue function and could therefore be a link to metabolic disorders. Circulating cytokine levels may also be increased in obesity and metabolic diseases. However, although fat distribution and inflammation are clearly linked to metabolic disorders, inflammatory gene expression in the different abdominal adipose depots has not been investigated in detail. The menopausal transition is followed by a centralization of body fat and increased adiposity. Notably, inflammatory changes in fat during the menopausal transition have not been characterized. Finally, there is a lack of studies investigating the long-term effects of weight loss on low-grade inflammation. The aim of this thesis was to characterize differences between fat depots and investigate putative changes in low-grade inflammation in adipose tissue and circulation following menopause or weight loss. Materials & Methods: The expression of inflammation-related genes was investigated in abdominal adipose tissue depots obtained from women with varying adiposity, before and after menopause or weight loss induced by surgery or dietary intervention. Circulating cytokine levels were analyzed using immunoassays. Results: Visceral fat displayed a distinct and adverse inflammatory profile compared with subcutaneous adipose tissues, and the higher gene expression in visceral fat was associated with adiposity. Postmenopausal women exhibited a higher expression of pro-inflammatory genes than premenopausal women that associated with central fat accumulation. There was also a menopause-related increase in circulating cytokine levels in postmenopausal women. After surgery-induced weight loss, there was a dramatic reduction in inflammatory gene expression followed by increased insulin sensitivity. We observed no alterations in circulating cytokine levels. Long-term dietary intervention, associated with weight loss, had favorable effects on inflammation in both adipose tissue and serum. Conclusion: Fat accumulation is linked to low-grade inflammation in abdominal adipose tissue. The unique inflammatory pattern of visceral fat suggests a distinct role in adipose tissue inflammation that is aggravated with increasing adiposity. In postmenopausal women, the adverse adipose inflammatory profile was associated with central fat accumulation, while higher circulating cytokine levels correlated with menopausal state/age. Our data from severely obese women undergoing surgery-induced weight loss clearly supports a link between adipose inflammation and insulin resistance. The long-term beneficial effects of weight loss were also demonstrated by the improved inflammatory profile after dietary intervention. In summary, excess body fat is clearly linked to adipose tissue inflammation. Long-term weight loss is accompanied by improved metabolic profile and reduced low-grade inflammation in fat.
|
|
| 2. |
- Kihlberg, Robert, 1975-
(author)
-
Influence in sensemaking during change : a study of the Swedish police reform and subsequent change work
- 2022
-
Doctoral thesis (other academic/artistic)abstract
- This dissertation is based on four articles, and examines efforts of influence in processes of sensemaking during, and subsequent to, the Swedish police reform. Sensemaking – a process where individuals work to understand what is going on when they encounter confusing events – is of central importance for how organizational change unfolds. Influence in sensemaking refers to activities with capacity to shape persons preferences and perceptions, as they try to make sense. In this dissertation, the overall purpose is to increase the understanding about influence in sensemaking during micro-processes of change. The research questions address how efforts of influence in sensemaking at different organizational levels can be understood, and by which means actors attempt to influence others sensemaking. The research applies sensemaking and sensegiving as its theoretical framework. With a qualitative approach, and ethnographic methods, influence in sensemaking is examined on four levels: Article 1 examines how the strategic level of reform promote change through visual media. The results show how 44 videos function to layer meaning and construct stereotypes in attempts to influence how the sensemaking of change would take place. Article 2 reports an ethnography of middle-managers involving employees in a multivocal process that aims to influence how sensemaking evolves. This process has four features: open-endedness, low control over cues given, several sources of cues, and the encouragement of complexity and ambiguity. Article 3 studies employees working as ‘change’ agents at employee conferences. Results show that these actors exert influence in three ways: drawing on positional power, acting powerless, and by attempts to give power away. Article 4 reports an ethnography of employees enacting a change initiative. The results show how employees’ various enactments involve episodic power, which influence the sensemaking process to varying degrees and towards diverse ends. Overall, the results from the articles outlines three understandings of influence in sensemaking: top-down efforts, reflexive interactions and responsive enactments – each with particular consequences for who can take part in processes of sensemaking, organizing, and change work, when and how. For organizations, the results imply a need for managers and employees to raise awareness of others as well as one’s own influence in sensemaking, as it shapes the way they make sense of, understand, and enact organizational change. In a larger perspective, this has significance for which forms of organizing are stimulated to develop – democratic or undemocratic.
|
|
