| 1. |
- Nagaraja, Ch., et al.
(author)
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Opening remarks
- 2016
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Conference paper (peer-reviewed)
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| 2. |
- Gerin, I., et al.
(author)
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ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto alpha-dystroglycan
- 2016
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7
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Journal article (peer-reviewed)abstract
- Mutations in genes required for the glycosylation of alpha-dystroglycan lead to muscle and brain diseases known as dystroglycanopathies. However, the precise structure and biogenesis of the assembled glycan are not completely understood. Here we report that three enzymes mutated in dystroglycanopathies can collaborate to attach ribitol phosphate onto a-dystroglycan. Specifically, we demonstrate that isoprenoid synthase domain-containing protein (ISPD) synthesizes CDP-ribitol, present in muscle, and that both recombinant fukutin (FKTN) and fukutin-related protein (FKRP) can transfer a ribitol phosphate group from CDP-ribitol to alpha-dystroglycan. We also show that ISPD and FKTN are essential for the incorporation of ribitol into alpha-dystroglycan in HEK293 cells. Glycosylation of alpha-dystroglycan in fibroblasts from patients with hypomorphic ISPD mutations is reduced. We observe that in some cases glycosylation can be partially restored by addition of ribitol to the culture medium, suggesting that dietary supplementation with ribitol should be evaluated as a therapy for patients with ISPD mutations.
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| 3. |
- Modin Larsson, Malin, 1980-, et al.
(author)
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Antibody prevalence and titer to norovirus (genogroup II) correlate with secretor (FUT2) but not with ABO phenotype or Lewis (FUT3) genotype
- 2006
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In: J Infect Dis. - : Oxford University Press. ; 194:10, s. 1422-1427
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Journal article (peer-reviewed)abstract
- BACKGROUND: Histo-blood group antigens and secretor status have been associated with susceptibility to Norovirus infections, which suggests that antibody prevalence and titer might correlate with these phenotypes. METHODS: Plasma samples (n = 105) from Swedish blood donors that had been genotyped for secretor (FUT2) and Lewis (Le; FUT3) genotypes and phenotyped for ABO and Le blood groups were analyzed for immunoglobulin G antibody prevalence and titers to norovirus genogroup (GG) II.4. RESULTS: The results showed that nonsecretors (se4128se428) and Lea+b- individuals not only had significantly lower antibody titers than did secretors (P < .0001) and Lea-b+ individuals (P < .0002) but were also significantly more often antibody negative (P < .05). Antibody titers in secretors were not significantly different between individuals of different Le (FUT3) genotypes or different ABO phenotypes. CONCLUSIONS: Nonsecretors and Lea+b- individuals are significantly less prone to be infected with GGII noroviruses. This new information extends previous knowledge and supports the hypothesis that nonsecretors are relatively but not absolutely resistant to norovirus infections.
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| 4. |
- Dahmani, Abdennasser, et al.
(author)
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Assessing the Efficacy of Improved Learning in Hourly Global Irradiance Prediction
- 2023
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In: Computers, Materials and Continua. - : Tech Science Press. - 1546-2218 .- 1546-2226. ; 77:2, s. 2579-2594
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Journal article (peer-reviewed)abstract
- Increasing global energy consumption has become an urgent problem as natural energy sources such as oil, gas, and uranium are rapidly running out. Research into renewable energy sources such as solar energy is being pursued to counter this. Solar energy is one of the most promising renewable energy sources, as it has the potential to meet the world’s energy needs indefinitely. This study aims to develop and evaluate artificial intelligence (AI) models for predicting hourly global irradiation. The hyperparameters were optimized using the Broyden-Fletcher-Goldfarb-Shanno (BFGS) quasi-Newton training algorithm and STATISTICA software. Data from two stations in Algeria with different climatic zones were used to develop the model. Various error measurements were used to determine the accuracy of the prediction models, including the correlation coefficient, the mean absolute error, and the root mean square error (RMSE). The optimal support vector machine (SVM) model showed exceptional efficiency during the training phase, with a high correlation coefficient (R = 0.99) and a low mean absolute error (MAE = 26.5741 Wh/m2), as well as an RMSE of 38.7045 Wh/m² across all phases. Overall, this study highlights the importance of accurate prediction models in the renewable energy, which can contribute to better energy management and planning.
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| 5. |
- Toledo, Alejandro Gomez, et al.
(author)
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O-Mannose and O-N-acetyl galactosamine glycosylation of mammalian alpha-dystroglycan is conserved in a region-specific manner
- 2012
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In: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 22:11, s. 1413-1423
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Journal article (peer-reviewed)abstract
- Defects in the O-linked glycosylation of the peripheral membrane protein alpha-dystroglycan (alpha-DG) are the main cause of several forms of congenital muscular dystrophies and thus the characterization of the glycosylation of alpha-DG is of great medical importance. A detailed investigation of the glycosylation pattern of the native alpha-DG protein is essential for the understanding of the biological processes related to human disease in which the protein is involved. To date, several studies have reported novel O-glycans and attachment sites on the mucin-like domain of mammalian alpha-DG with both similar and contradicting glycosylation patterns, indicating the species-specific O-glycosylation of mammalian alpha-DG. By applying a standardized purification scheme and subsequent glycoproteomic analysis of native alpha-DG from rabbit and human skeletal muscle biopsies and from cultured mouse C2C12 myotubes, we show that the O-glycosylation patterns of the mucin-like domain of native alpha-DG are conserved among mammalians in a region-specific manner.
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