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1.	Bigotti, MG, et al. (author) The α-dystroglycan N-terminus is a broad-spectrum antiviral agent against SARS-CoV-2 and enveloped viruses 2024 In: Antiviral research. - 1872-9096. ; 224, s. 105837- Journal article (peer-reviewed)
2.	Feng, L, et al. (author) Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription 2012 In: BioTechniques. - : Future Science Ltd. - 1940-9818 .- 0736-6205. ; 52:4, s. 263- Journal article (peer-reviewed)abstract Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses. We report a simple RT-PCR-based assay for strand-specific gene-expression analysis. By modifying the cDNA sequence during reverse transcription, the opposite strands of target sequences can be simultaneously detected by postamplification melting curve analysis and primer-initiated transcripts are readily distinguished from nonspecifically primed cDNA. We have utilized this technique to optimize the specificity of reverse transcription on a panel of 15 target genes. Primer-independent reverse transcription occurred for all target sequences when reverse transcription was performed at 42°C and accounted for 11%–57% of the final PCR amplification products. By raising the reaction temperature to 55°C, the specificity of reverse transcription could be increased without significant loss of sensitivity. We have also demonstrated the utility of this technique for analysis of (+) and (-) RNA synthesis of influenza A virus in infected cells. Thus, this technique represents a powerful tool for analysis of bidirectional RNA synthesis.
3.	Rosendal, Ebba, et al. (author) Low-pathogenic chimeric viruses to study the structural proteins of tick-borne encephalitis virus Other publication (other academic/artistic)
4.	Soderholm, S, et al. (author) Immuno-modulating properties of saliphenylhalamide, SNS-032, obatoclax, and gemcitabine 2016 In: Antiviral research. - : Elsevier BV. - 1872-9096 .- 0166-3542. ; 126, s. 69-80 Journal article (peer-reviewed)

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Type of publication: journal article (3); other publication (1)

Type of content: peer-reviewed (3); other academic/artistic (1)

Author/Editor: Anastasina, M (3); Stenman, J (2); Kainov, D (2); Feng, L. (1); Nilsson, Emma (1); Islam, MM (1); show more...; Andersson, S (1); Erdmann, M (1); Ahlgren, Ulf (1); Vapalahti, O. (1); Lindgren, Marie (1); Överby, Anna K. (1); Haglund, C (1); Willekens, Stefanie ... (1); Merits, Andres (1); Saelens, X. (1); Katajisto, P (1); Balistreri, G (1); Julkunen, I (1); Bigotti, MG (1); Klein, K (1); Gan, ES (1); Davidson, AD (1); Butcher, SJ (1); Collinson, I (1); Ooi, EE (1); Brancaccio, A (1); Yamauchi, Y (1); Paju, A (1); Soderholm, S (1); Stenman, UH (1); Rosendal, Ebba (1); Morini, Federico (1); Tynell, J (1); Matikainen, S (1); Lintula, S (1); Ho, TH (1); Hotakainen, K (1); Orpana, A (1); Sauliene, I. (1); Butcher, Sarah J (1); Svoboda, Pavel (1); Nyman, TA (1); Anastasina, Maria (1); Bisikalo, Kyrylo (1); Holoubek, Jiří (1); Samuelsson, Ebba (1); Nordén, Rickard (1); Růžek, Daniel (1); Poranen, MM (1); show less...

University: Karolinska Institutet (3); Umeå University (1)

Language: English (4)

Research subject (UKÄ/SCB): Medical and Health Sciences (1)

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