| 1. |
- Aslan, Sema, et al.
(author)
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Development of a Bioanode for Microbial Fuel Cells Based on the Combination of a MWCNT-Au-Pt Hybrid Nanomaterial, an Osmium Redox Polymer and Gluconobacter oxydans DSM 2343 Cells
- 2017
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In: ChemistrySelect. - : Wiley. - 2365-6549. ; 2:36, s. 12034-12040
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Journal article (peer-reviewed)abstract
- In this work, a carbon felt electrode (CFE) was modified with a multiwalled carbon nanotube-gold-platinum (MWCNT-Au-Pt) hybrid nanomaterial and integrated with an osmium redox polymer (OsRP, [Os(2, 2’-bipyridine)2(poly-vinylimidazole)10Cl]Cl) and Gluconobacter oxydans DSM 2343 (G. oxydans) cells. The developed electrode was used as the bioanode in a 5.0 mM K3Fe(CN)6mediator containing phosphate buffer (pH 6.5) anolyte and combined with a Pt wire cathode in phosphoric acid medium (pH 3.5). As a result, a two chamber microbial fuel cell (MFC) was formed, in which an activated Nafion membrane was used as a proton exchange membrane. The OsRP/G.oxydans/MWCNT-Au-Pt/CFE based bioanode was electrochemically examined in differently deoxygenated bioanode chambers and additionally the amounts of hybrid nanomaterial and OsRP were optimized. In terms of MFC characteristics, it was found that an anaerobic OsRP/G.oxydans/MWCNT-Au-Pt/CFE bioanode based MFC had a maximum power density of 32.1 mW m-2(at 90 mV), a maximum current density of 1032 mA m-2and a charge transfer efficiency (E%) value of 22.30 (open circuit potential 180 mV).
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| 2. |
- Aslan, Sema, et al.
(author)
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Development of an Osmium Redox Polymer Mediated Bioanode and Examination of Its Performance in Gluconobacter oxydans Based Microbial Fuel Cell
- 2017
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In: Electroanalysis. - : Wiley. - 1040-0397. ; 29:6, s. 1651-1657
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Journal article (peer-reviewed)abstract
- Gluconobacter oxydans (G. oxydans) cells together with an osmium redox polymer (ORP) [Osmium (2,2'-bipyridine)2(poly-vinylimidazole)10Cl]Cl were combined with a glassy carbon paste electrode (GCPE) to form a bioanode for a microbial fuel cell (MFC) based on G. oxydans. Although there are G.oxydans/ ORP combined bioanode in the literature, as far as it is known, this system is the first one where G.oxydans/ORP bioanode is combined with a cathode and a MFC is formed. After the optimization of experimental parameters, analytical characteristics of ORP/G. oxydans/GCPE bioanode were investigated. ORP/G. oxydans/GCPE showed two linear ranges for ethanol substrate as 1.0-30mM (R2=0.902) and 30-500mM (R2=0.997) and analytical range as 1.0-1000mM. Limit of detection (3.0s/m) and limit of quantification (10s/m) values were calculated as 1.29mM and 4.30mM respectively where the RSD value was 1.16% for n=5. Combining the developed bioanode in the presence of 5.0mM K3Fe(CN)6 mediator with a Pt wire cathode a double compartment MFC was obtained via a salt bridge. G. oxydans/GCPE bioanode based MFC had maximum power density of 0.133 μW cm-2 (at 33.5 mV), maximum current density as 8.73 μA cm-2 and OCP value of 156 mV. On the other hand, ORP/G. oxydans/GCPE based MFC showed maximum power density as 0.26 μW cm-2 (at 46.8 mV), maximum current density as 15.079 μA cm-2 and OCP value of 176 mV.
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| 3. |
- Sayhi, Maher, et al.
(author)
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Electrochemical detection of influenza virus H9N2 based on both immunomagnetic extraction and gold catalysis using an immobilization-free screen printed carbon microelectrode
- 2018
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In: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 107, s. 170-177
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Journal article (peer-reviewed)abstract
- Influenza is a viral infectious disease considered as a source of many health problems and enormous socioeconomic disruptions. Conventional methods are inadequate for in-field detection of the virus and generally suffer from being laborious and time-consuming. Thus, studies aiming to develop effective alternatives to conventional methods are urgently needed. In this work, we developed an approach for the isolation and detection of influenza A virus subtype H9N2. For this aim, two specific influenza receptors were used. The first, anti-matrix protein 2 (M2) antibody, was attached to iron magnetic nanoparticles (MNPs) and used for the isolation of the virus from allantoic fluid. The second biomolecule, Fetuin A, was attached to an electrochemical detectable label, gold nanoparticles (AuNPs), and used to detect the virus tacking advantage from fetuin-hemagglutinin interaction. The MNP-Influenza virus-AuNP formed complex was isolated and treated by an acid solution then the collected gold nanoparticles were deposited onto a screen printed carbon electrode. AuNPs catalyzes the hydrogen ions reduction in acidic medium while applying an appropriate potential, and the generated current signal was proportional to the virus titer. This approach allows the rapid detection of influenza virus A/H9N2 at a less than 16 HAU titer.
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| 4. |
- Timur, Suna, et al.
(author)
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Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes
- 2007
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In: Electrochemistry Communications. - : Elsevier BV. - 1388-2481. ; 9:7, s. 1810-1815
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Journal article (peer-reviewed)abstract
- Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature were also examined. After the optimisation studies, the system was characterised by using glucose as substrate. Moreover, the microbial biosensor was also prepared by using phenol adapted bacteria and then, calibrated to phenol. After that, it was applied for phenol detection in an artificial waste water sample. (C) 2007 Published by Elsevier B.V.
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