| 1. |
- Danmark, Staffan, et al.
(author)
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Osteogenic Differentiation by Rat Bone Marrow Stromal Cells on Customized Biodegradable Polymer Scaffolds
- 2010
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In: Journal of bioactive and compatible polymers (Print). - : SAGE Publications. - 0883-9115 .- 1530-8030. ; 25:2, s. 207-223
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Journal article (peer-reviewed)abstract
- In this report, poly(L-lactide-co-epsilon-caprolactone), poly(LLA-co-CL) and poly(L-lactide-co-1,5-dioxepan-2-one), poly(LLA-co-DXO) were evaluated and compared for potential use in bone tissue engineering constructs together with bone marrow stromal cells (BMSC). The copolymers were tailored to reduce the level of harmful tin residuals in the scaffolding. BMSC isolated from Sprague-Dawley rats were seeded onto the scaffolds and cultured in vitro for up to 21 days. Cell spreading and proliferation was analyzed after 72 h by scanning electron microscopy and thiazolyl blue tetrazolium bromide (MTT) conversion assay. Osteogenic differentiation of BMSC was evaluated by real-time PCR after 14 and 21 days of culture. Hydrophilicity was significantly different between poly(LLA-co-CL) and poly(LLA-co-DXO) with the latter being more hydrophilic. After 72 h, both scaffolds supported increased cell proliferation and the mRNA expression of osteocalcin and osteopontin was significantly increased after 21 days. Further investigation of these constructs, with lower levels of tin residuals, are being pursued.
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| 2. |
- Hildingsson, Ingegerd, 1955-, et al.
(author)
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First-Time Parents’ Perception of Antenatal Education in Sweden
- 2013
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In: International Journal of Childbirth. - : Springer Publishing Company. - 2156-5287 .- 2156-5295. ; 3:1, s. 28-38
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Journal article (peer-reviewed)abstract
- Antenatal parent education is one part of the parenthood support program and is offered to all firsttime parents in Sweden. The aim was to explore first-time parents' experiences of antenatal education and its helpfulness in preparation for birth and early parenthood. Eight hundred ten first-time parents completed questionnaires. The antenatal education was a positive experience for 56% and 62% of women and men, respectively. Antenatal education was helpful during birth for 68% of women and 63% of men. Forty-seven percent of women and 44% of men found it helpful for early parenthood. High level of education was the only background variable associated with being satisfied with antenatal education. Satisfaction was lower than reported in previous studies and indicates room for change and improvement in antenatal parent education.
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| 3. |
- Idris, Shaza B., et al.
(author)
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Biocompatibility of Polyester Scaffolds with Fibroblasts and Osteoblast-like Cells for Bone Tissue Engineering
- 2010
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In: Journal of bioactive and compatible polymers (Print). - : SAGE Publications. - 0883-9115 .- 1530-8030. ; 25:6, s. 567-583
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Journal article (peer-reviewed)abstract
- The aim of this study was to evaluate the in vitro cytotoxicity and cytocompatibility of the developed aliphatic polyester co-polymer scaffolds: poly(L-lactide-co-epsilon-caprolactone) and poly(L-lactide-co-1,5-dioxepan-2-one). The scaffolds were produced by solvent casting and particulate leaching, and tested by direct and indirect contact cytotoxicity assays on human osteoblast-like cells and mouse fibroblasts. Cell morphology was documented by light and scanning electron microscopy. Viability was assessed by the MTT, neutral red uptake, lactic dehydrogenase and apoptosis assays. Extraction tests confirmed that the scaffolds did not have a cytotoxic effect on the cells. The cells grew and spread well on the test scaffolds with good cellular attachment and viability. The scaffolds are noncytotoxic and biocompatible with the two cell types and warrant continued investigation as potential constructs for bone tissue engineering.
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| 4. |
- Idris, Shaza Bushra, et al.
(author)
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Polyester copolymer scaffolds enhance expression of bone markers in osteoblast-like cells
- 2010
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In: J BIOMED MATER RES PART A. - : Wiley. - 1549-3296. ; 94A:2, s. 631-639
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Journal article (peer-reviewed)abstract
- In tissue engineering, the resorbable aliphatic polyester poly(L-lactide) (PLLA) is used as scaffolds in bone regeneration. Copolymers of poly(L-lactide)-co-(epsilon-caprolactone) [poly(LLA-co-CL)] and poly(L-lactide)-co-(1,5-dioxepan-2-one) [poly(LLA-co-DXO)], with superior mechanical properties to PLLA, have been developed to be used as scaffolds, but the influence on the osteogenic potential is unclear. This in vitro study of test scaffolds of poly(LLA-co-CL) and poly(LLA-co-DXO) using PLLA scaffolds as a control demonstrates the attachment and proliferation of human osteoblast-like cells (HOB) as measured by SEM and a methylthiazol tetrazolium (MTT) colorimetric assay, and the progression of HOB osteogenesis for up to 3 weeks; expressed as synthesis of the osteoblast differentiation markers: collagen type 1 (Col 1), alkaline phosphatase, bone sialoprotein, osteocalcin (OC), osteopontin and runt related gene 2 (Runx2). Surface analysis disclosed excellent surface attachment, spread and penetration of the cells into the pores of the test scaffolds compared to the PLLA. MTT results indicated that test scaffolds enhanced the proliferation of HOBs. Cells grown on the test scaffolds demonstrated higher synthesis of Col 1 and OC and also increased bone markers mRNA expression. Compared to scaffolds of PLLA, the poly(LLA-co-CL) and poly(LLA-co-DXO) scaffolds enhanced attachment, proliferation, and expression of osteogenic markers by HOBs in vitro. Therefore, these scaffolds might be appropriate carriers for bone engineering. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 631-639, 2010
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| 5. |
- Jemt, Torsten, 1950, et al.
(author)
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Implant-supported welded titanium frameworks in the edentulous maxilla: a 5-year prospective multicenter study.
- 2002
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In: The International journal of prosthodontics. - 0893-2174. ; 15:6, s. 544-8
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Journal article (peer-reviewed)abstract
- PURPOSE: This study evaluated the 5-year clinical and radiographic performance of fixed implant-supported maxillary prostheses with either welded titanium or conventional cast-gold alloy frameworks. MATERIALS AND METHODS: Fifty-eight consecutive patients were provided with 349 osseointegrated Brånemark system implants in the edentulous maxilla at six different implant centers. Twenty-eight of the patients received, at random, prostheses with laser-welded titanium frameworks, and the remaining 30 patients had prostheses with conventional cast-gold alloy frameworks. Clinical and radiographic data were collected for 5 years after prosthesis placement. RESULTS: The titanium and cast-gold framework groups exhibited similar cumulative survival and success rates (CSR). The 5-year implant CSR from time of placement was 91.4% and 94.0%, respectively, and from prosthesis delivery the rate was 94.9% and 95.6%, respectively. The corresponding 5-year prosthesis CSRs were 96.4% and 93.3%. One patient from each group lost all the implants and turned to complete dentures within the first year of function. Another patient with a cast-gold framework had the prosthesis replaced after 4 years, basically because of problems with the veneering material. No fractures of implant components were observed during the follow-up period. Bone loss was on average 0.59 mm (SD 0.97 mm) during 5 years, with no statistical difference between the two groups. CONCLUSION: Welded titanium frameworks presented a similar favorable clinical performance as conventional cast-gold alloy frameworks in fixed implant-supported prostheses in the edentulous maxilla after 5 years in function. Implant failures were concentrated in only a few patients in each study group.
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| 9. |
- Mustafa, Kamal, et al.
(author)
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The influence of surface topography of ceramic abutments on the attachment and proliferation of human oral fibroblasts.
- 2005
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In: Biomaterials. - : Elsevier BV. - 0142-9612. ; 26:4, s. 373-81
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Journal article (peer-reviewed)abstract
- As different implant abutments are introduced to obtain a sufficient soft tissue barrier, the aim of this study was to determine the effect of three different surface modifications of densely sintered high-purity aluminium oxide on morphology, attachment and proliferation of human gingival fibroblasts. Fibroblasts were cultured on pressed aluminium oxide, milled, and then sintered to full density (1), on pressed, densely sintered (2), and on pressed, densely sintered and then polished surfaces (3). The different surfaces were analyzed using a confocal laser scanner, an atomic force microscope and a scanning electron microscope. The cell profile areas were measured using a semiautomatic interactive image analyzer and the figures were expressed as percent of attachment. The polished specimens had the smoothest surfaces and the roughest were the milled surfaces in terms of height deviation. No difference was found in the spacing between the peaks on the polished surfaces compared to the milled surfaces. Fibroblasts on the milled ceramic appeared to follow the direction of the fine irregularities on the surface. The analyses showed the polished surfaces had significantly higher percentages of initial cell attachment than the other surfaces (P < 0.05). After 3 days of cell culture, significantly more cells were attached to the milled and sintered surfaces than to the polished one, possibly indicating higher proliferation capacity on those types of surfaces.
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| 10. |
- Schander, Kerstin, et al.
(author)
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Response of Bone and Periodontal Ligament Cells to Biodegradable Polymer Scaffolds In Vitro
- 2010
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In: Journal of bioactive and compatible polymers (Print). - : SAGE Publications. - 0883-9115 .- 1530-8030. ; 25:6, s. 584-602
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Journal article (peer-reviewed)abstract
- In this in vitro study, the initial response of human periodontal ligament (PDL) cells and alveolar osteoblast-like cells (HOB) to three biodegradable polymers with varying pore size and different mechanical properties were evaluated. Scaffolds were synthesized from poly(L-lactide), [poly(LLA)], poly(L-lactide-co-1,5-dioxepan-2-one), [poly(LLA-co-DXO)], poly(L-lactide-co-epsilon-caprolactone), and [poly(LLA-co-CL)] with pore sizes greater or less than 90 mm by salt leaching. Cells were obtained from patients undergoing routine oral surgery. After 2-4 passages, the cells were grown on scaffolds and in culture plates (control) for 3 h (PDL cells), 3 days (PDL cells and HOB), 10 and 14 days (HOB), respectively. The cellular morphology and spreading were determined by scanning electron microscopy (SEM) and the attachment and proliferation were evaluated by MTT assays. The SEM images revealed heterogeneous cellular morphology and good spreading. Cellular attachment and proliferation were significantly higher on poly(LLA-co-DXO) and poly(LLA-co-CL) than on poly(LLA) scaffolds (p = 0.003) and highest for poly(LLA-co-DXO). Expression of bone formation markers, collagen-I (COL-I), transforming growth factor-beta(1) (TGF-beta(1)), and osteocalcin (OCN), was determined by ELISA. The expression of COL-1 was similar for HOB and PDL cells, but significantly higher for pore size >90 mm while the HOB expression of TGF-beta(1) and OCN was greater on poly(LLA-co-CL) and poly(LLA-co-DXO) than on poly(LLA) scaffolds.
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