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- Ahmed, Abdulla, et al.
(author)
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Elevated plasma sRAGE and IGFBP7 in heart failure decrease after heart transplantation in association with haemodynamics
- 2020
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In: ESC Heart Failure. - : Wiley. - 2055-5822. ; 7:5, s. 2340-2353
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Journal article (peer-reviewed)abstract
- AIMS: Metabolic derangement is implicated in the pathophysiology of heart failure (HF) and pulmonary hypertension (PH). We aimed to identify the dynamics of metabolic plasma proteins linked to end-stage HF and associated PH in relation to haemodynamics, before and after heart transplantation (HT).METHODS AND RESULTS: Twenty-one metabolic plasma proteins were analysed with proximity extension assay in 20 controls and 26 patients before and 1 year after HT. Right heart catheterizations were performed in the HF patients pre-operatively and 1 year after HT. Plasma levels of soluble receptor for advanced glycation end products (sRAGE) and insulin-like growth factor-binding protein 7 (IGFBP7) were higher in HF patients compared with controls (P < 0.0001) and decreased after HT (P < 0.0001), matching controls' levels. The decrease in sRAGE after HT correlated with improved mean pulmonary arterial pressure (rs = 0.7; P < 0.0001), pulmonary arterial wedge pressure (rs = 0.73; P < 0.0001), pulmonary vascular resistance (rs = 0.65; P = 0.00062), and pulmonary arterial compliance (rs = -0.52; P = 0.0074). The change in plasma IGFBP7 after HT correlated with improved mean right atrial pressure (rs = 0.71; P = 0.00011) and N-terminal pro-brain natriuretic peptide (rs = 0.71; P < 0.0001).CONCLUSIONS: Our results indicate that plasma sRAGE may reflect passive pulmonary vascular congestion and the 'mechanical' state of the pulmonary vasculature in HF patients with or without related PH. Furthermore, sRAGE and IGFBP7 may provide additional insight into the pathophysiological mechanisms in HF and associated PH. Their potential clinical and therapeutic relevance in HF and associated PH need further investigation.
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- Ahmed, Abdulla, et al.
(author)
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Prolargin and matrix metalloproteinase-2 in heart failure after heart transplantation and their association with haemodynamics
- 2020
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In: ESC Heart Failure. - : Wiley. - 2055-5822. ; 7:1, s. 224-235
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Journal article (peer-reviewed)abstract
- AIMS: Remodelling of the extracellular matrix (ECM) is a key mechanism involved in the development and progression of heart failure (HF) but also functional in associated pulmonary hypertension (PH). Our aim was to identify plasma ECM proteins associated to end-stage HF and secondary PH in relation to haemodynamics, before and after heart transplantation (HT).METHODS AND RESULTS: Twenty ECM plasma proteins were analysed with proximity extension assay in 20 controls and 26 HF patients pre-HT and 1 year post-HT. Right heart catherization haemodynamics were assessed in the patients during the preoperative evaluation and at the 1 year follow-up post-HT. Plasma levels of prolargin and matrix metalloproteinase-2 (MMP-2) were elevated (P < 0.0001) in HF patients compared with controls and decreased (P < 0.0001) post-HT towards controls' levels. The decrease in prolargin post-HT correlated with improved mean right atrial pressure (rs = 0.63; P = 0.00091), stroke volume index (rs = -0.73; P < 0.0001), cardiac index (rs = -0.64; P = 0.00057), left ventricular stroke work index (rs = -0.49; P = 0.015), and N-terminal pro brain natriuretic peptide (rs = 0.7; P < 0.0001). The decrease in MMP-2 post-HT correlated with improved mean pulmonary artery pressure (rs = 0.58; P = 0.0025), mean right atrial pressure (rs = 0.56; P = 0.0046), pulmonary artery wedge pressure (rs = 0.48; P = 0.016), and N-terminal pro brain natriuretic peptide (rs = 0.56; P = 0.0029).CONCLUSIONS: The normalization pattern in HF patients of plasma prolargin and MMP-2 post-HT towards controls' levels and their associations with improved haemodynamics indicate that prolargin and MMP-2 may reflect, in part, the aberrant ECM remodelling involved in the pathophysiology of HF and associated PH. Their potential clinical use as biomarkers or targets for future therapy in HF and related PH remains to be investigated.
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3. |
- Andersson, Mattias K, 1979, et al.
(author)
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The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response
- 2008
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In: BMC Cell Biology. - : Springer Science and Business Media LLC. - 1471-2121. ; 9
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Journal article (peer-reviewed)abstract
- BACKGROUND: FUS, EWS and TAF15 are structurally similar multifunctional proteins that were first discovered upon characterization of fusion oncogenes in human sarcomas and leukemias. The proteins belong to the FET (previously TET) family of RNA-binding proteins and are implicated in central cellular processes such as regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. In the present study, we investigated the expression and cellular localization of FET proteins in multiple human tissues and cell types. RESULTS: FUS, EWS and TAF15 were expressed in both distinct and overlapping patterns in human tissues. The three proteins showed almost ubiquitous nuclear expression and FUS and TAF15 were in addition present in the cytoplasm of most cell types. Cytoplasmic EWS was more rarely detected and seen mainly in secretory cell types. Furthermore, FET expression was downregulated in differentiating human embryonic stem cells, during induced differentiation of neuroblastoma cells and absent in terminally differentiated melanocytes and cardiac muscle cells. The FET proteins were targeted to stress granules induced by heat shock and oxidative stress and FUS required its RNA-binding domain for this translocation. Furthermore, FUS and TAF15 were detected in spreading initiation centers of adhering cells. CONCLUSION: Our results point to cell-specific expression patterns and functions of the FET proteins rather than the housekeeping roles inferred from earlier studies. The localization of FET proteins to stress granules suggests activities in translational regulation during stress conditions. Roles in central processes such as stress response, translational control and adhesion may explain the FET proteins frequent involvement in human cancer.
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5. |
- Arvidsson, Anna K, 1971-, et al.
(author)
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Appraisal methodology : Deliverable D2.1
- 2023
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Reports (other academic/artistic)abstract
- This report represents INFRACOMS deliverable D2.1 Appraisal Methodology. It builds upon the deliverables of INFRACOMS Work Package 1 which identified the information needs, gaps and priorities of NRAs in terms of their approach to data collection and monitoring, and a list of current and emerging measurement technologies. This report includes a review of several commonly-used appraisal methodologies that can be used to evaluate the effectiveness, suitability and potential impact of new technologies for an organisation. These methodologies include Technology Readiness Levels (TRLs), Cost Benefit Analysis (CBA), Life Cycle Cost Analysis (LCCA), Risk Assessment, and Multi-Criteria Decision Analysis (MCDA). Elements of these commonly used methodologies are included in the INFRACOMS Appraisal Methodology. The report also includes key highlights from a workshop with NRAs conducted in January 2023 which also fed into the design of the appraisal methodology. The INFRACOMS Appraisal Methodology described here is designed around the technology use case, that is, a particular application of a technology by a NRA. It incorporates three core processes for Pre-Evaluation, Evaluation and Case Studies of technology use cases. It also includes processes for NRAs to define their strategic and technical priorities so that the appraisal process can be tailored to addressing their individual requirements, as identified from Work Package 1.
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6. |
- Arvidsson, Anna K, 1971-, et al.
(author)
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Current Practice, Future need and Gap Analysis : Deliverable D1.1
- 2023
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Reports (other academic/artistic)abstract
- This report is INFRACOMS first deliverable D1.1. It addresses the “Understanding of information needs and gaps” component of the project. The aim has been to identify the current priorities and future needs of NRAs for the management of carriageway and bridge assets, specifically in terms of their approach to data collection and monitoring. The approach has been to establish existing knowledge via a review of previous projects, current best practices and standards in data collection and inspection, and a review of current business processes, NRA strategies around data collection and digitalisation etc. The report identifies a set of key imperatives for carriageway and bridge assets covering Availability, Reliability, Environment, Economy and Safety. Each of these is supported by the collection of key condition data, which is used to report technical parameters and performance indicators that can be combined to assess the ability of the asset to meet its key imperatives. A wide range of technologies are identified, which are currently applied to collect the data that supports this assessment.The consultation shows that there are also gaps between the desired and the current capability for the assessment of these assets. These include gaps in the data, challenges in the ability to collect the data, gaps in the application of the data that is already collected etc. A review of emerging technologies shows that there are tools and technologies that could help to fill these gaps. These could overcome the limitations of current technologies, better integrate new data sources, provide greater flexibility in using current and new data, and provide better analysis. They include remote sensing, Internet of Things (IoT), crowdsourcing, and advanced data processing/visualisation.
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7. |
- Arvidsson, Anna K, 1971-, et al.
(author)
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Report on state-of-the-art data assessment and visualisation methods : Deliverable D3.1
- 2023
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Reports (other academic/artistic)abstract
- Effective analysis and visualisation of data is critical for the efficient application of the data provided by carriageway and bridge condition monitoring technologies. It supports better decisions in relation to asset reliability, availability, safety, economy and environment. This report discusses the link between the data provided by monitoring technologies on the properties of assets and how the collected data can be analysed and visualised to provide value in decision support. The next step in the report is to use this understanding to develop an appraisal system which could enable technologies in the INFRACOMS technology database to be appraised (scored) in relation to their abilities for data analysis, visualisation, integration and use in decision support.The presented system is referred to as the D3.1 scoring system. It consists of four components covering data visualisation, data analysis, integration within current data architectures and potential for practical decision-making. The present D3.1 report primarily examines the components pertaining to data visualisation and data analysis, while the exploration of the other two components, data architecture and decision support, will be carried out in the D3.2 report. It is proposed that the D3.1 scoring system could be used to appraise the capability of monitoring technologies to support asset management decisions, and would become an integral component of the INFRACOMS Appraisal Toolkit. It will also be used to further filter the current INFRACOMS Technology Database 2.0 technologies as part of the Appraisal Toolkit as INFRACOMS completes the development of the toolkit/database within WP2.
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8. |
- Arvidsson, Håkan, 1962-, et al.
(author)
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Användning av begreppet bergtyp i Sverige : delrapport 1 för ny klassificering av bergtyp i Sverige
- 2022
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Reports (other academic/artistic)abstract
- Idag klassificeras bergtyp i tre klasser med hjälp av provningsmetoden kulkvarn (under 18, 18–30 eller över 30 procent). Sedan 2004, då Sverige började följa europeiska produktstandarder (SS-EN) har inte kulkvarn använts som kravparameter för de flesta materialapplikationer med krav på ballast vid byggande. Projektet går ut på att försöka förnya definitioner och gränser för olika nivåer för klassificering av berghållfasthet. Metodiken för att definiera bergtyp ska gärna vara någorlunda lättillgänglig. I denna, första, delrapport redovisas hur begreppet bergtyp används i Sverige. Det har undersökts i krav- och upphandlingsdokument samt hur och i vilken omfattning branschens aktörer använder begreppet (bergtyp). Ett antal av personer med olika roller som konsulter, beställarombud, myndigheter, laboratorier och lärosäten har svarat på enkät eller intervjuats. AMA Anläggning 20 är det kravdokument som främst använder begreppet bergtyp. Sedan finns det en del andra dokument som gör hänvisningar till AMA Anläggning och därigenom indirekt använder begreppet. Förutom för vissa konsulter och vissa på Trafikverket är intresset och användandet generellt lågt av begreppet bergtyp. Intresset är större för ett nytt system av bergtyp än det är för det nuvarande bland de som har lämnat svar. Där efterfrågas också ofta ökad användbarhet.
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9. |
- Arvidsson, Håkan, 1962-, et al.
(author)
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Bergklassificering för anläggningsändamål i andra länder : delrapport 2 för ny klassificering av bergtyp i Sverige
- 2022
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Reports (other academic/artistic)abstract
- Idag klassificeras bergtyp i tre klasser med hjälp av provningsmetoden kulkvarn (under 18, 18–30 eller över 30 procent). Sedan 2004, då Sverige började följa europeiska produktstandarder (SS-EN), har inte kulkvarn använts som kravparameter för de flesta materialapplikationer med krav på ballast vid byggande. Projektet går ut på att försöka förnya definitioner och gränser för olika nivåer för klassificering av berghållfasthet. Metodiken för att definiera bergtyp ska gärna vara någorlunda lättillgänglig. I denna, andra, delrapport redovisas hur bergmaterial klassificeras i andra länder. Det har undersökts genom att studera nationella och europeiska standarder som berör materialklassificering. Många nationella klassificeringar har erhållits genom förfrågningar till projektgruppens kontakter och bland kontakternas kontakter. Svaren från kontakter har bestått av nationella standarder/kravdokument/handböcker etcetera. Dessa har bifogats eller skickats som sammanfattningar i e-post i form av text och/eller tabeller. Klassificering i de standarder som studerats har inga tydliga eller självklara förslag med motsvarande syfte som vårt (Sveriges) system med bergtyp. Principen för det franska/belgiska klassificeringssystemet är tilltalande men nivåerna bör nog ses över för svenska förhållanden och användningsområden. Deras system bygger på micro-Deval, Los Angeles och polervärde. Polervärde är inte något vi behöver ta fasta på i Sverige. Det är en egenskap som testas endast för slitlager. Men att använda laboratoriemetoder som är vanliga i Sverige, som micro-Deval och Los Angeles, är tilltalande. Ett riktvärde för summan (maximi- eller minimivärde) av valda egenskaper är också intressant
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10. |
- Arvidsson, Håkan, 1962-, et al.
(author)
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Bergtypsklassificering i Sverige : förslag på nytt system
- 2023
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Reports (other academic/artistic)abstract
- Projektet ska föreslå ett nytt system för bergtypsklassificering för användning i Sverige. Syftet med projektet är att ta fram underlag till ett klassificeringssystem för bergmaterial i bergtyper utifrån dess användbarhet vid anläggningsbyggande. Det nya systemet är tänkt att ersätta dagens system med indelning i bergtyp efter kulkvarnsvärde. Systemet ska bygga på egenskaper definierade av provningsmetoder som är beskrivna i europeiska metodstandarder. Klassificeringssystemet ska både bedöma bergmaterialets nötningsegenskaper och dess hållfasthetsegenskaper (motstånd mot krossning). Den metodik som använts i projektet är enkäter och intervjuer med aktörer i branschen samt studier av krav- och upphandlingsdokument för att reda ut hur användning av nuvarande (år 2022) klassificeringssystem används och vilka behov som finns. Omvärldsbevakning har gjorts i andra länder (i huvudsak Europa) för att få ny kunskap och inspiration till ett nytt klassificeringssystem. Analys av bergmaterialsdata har gjorts för att se på relevanta samband och för att se på konsekvenser av hypotetiska klassificeringssystem inför fastställande av nya nivåer. Inom detta projekt finns mer än 2 000 dataposter av bergmaterial. Dataposterna består av petrografisk beskrivning, resultat från kulkvarn, micro-Deval och Los Angeles-tester. Data har analyserats för att beräkna en generell korrelation mellan micro-Deval och kulkvarn. Det föreslagna systemet har mest påverkan på främst spröda graniter som kan komma att klassificeras sämre än i tidigare bergtypsklassificeringssystem. Detta projekt föreslår ett nytt klassificeringssystem som bygger på provning av nötning och hållfasthet, micro-Deval (MDE) och Los Angeles (LA). Klassificeringsnivåerna är grundade efter förmodad användbarhet för olika anläggningsmaterial.
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