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- Tyrode, E, et al.
(author)
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A vibrational sum frequency spectroscopy study of the liquid-gas interface of acetic acid-water mixtures: 2. Orientation analysis
- 2005
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In: Journal of Physical Chemistry B. - 1520-6106 .- 1520-5207. ; 109, s. 329-341
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Journal article (peer-reviewed)abstract
- Vibrational sum frequency spectroscopy has been used to investigate the surface of aqueous acetic acid solutions. By studying the methyl and carbonyl vibrations with different polarization combinations, an orientation analysis of the acetic acid molecules has been performed in the concentration range 0-100%. The surface tension of acetic acid solutions was also measured in order to obtain the surface concentration. The orientation of the interfacial acetic acid molecules was found to remain essentially constant in an upright position with the methyl group directed toward the gas phase in the whole concentration range. The tilt angle (CH3) of the symmetry axis of the methyl group with respect to the surface normal was found to be lower than 15 when considering a distribution of angles or as narrow as 0 ± 11 when assuming a Gaussian distribution. Further investigations showed that the C=O bond tilt (C=O) of the acetic acid hydrated monomer was constant and close to 55 in the concentration range where it was detected. Finally, the orientation information is discussed in terms of different species of acetic acid, where the formation of a surface layer of acetic acid cyclic dimers is proposed at high acid concentrations.
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- Zago, E, et al.
(author)
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Early downregulation of hsa-miR-144-3p in serum from drug-naïve Parkinson's disease patients
- 2022
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In: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 12:1, s. 1330-
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Journal article (peer-reviewed)abstract
- Advanced age represents one of the major risk factors for Parkinson’s Disease. Recent biomedical studies posit a role for microRNAs, also known to be remodelled during ageing. However, the relationship between microRNA remodelling and ageing in Parkinson’s Disease, has not been fully elucidated. Therefore, the aim of the present study is to unravel the relevance of microRNAs as biomarkers of Parkinson’s Disease within the ageing framework. We employed Next Generation Sequencing to profile serum microRNAs from samples informative for Parkinson’s Disease (recently diagnosed, drug-naïve) and healthy ageing (centenarians) plus healthy controls, age-matched with Parkinson’s Disease patients. Potential microRNA candidates markers, emerging from the combination of differential expression and network analyses, were further validated in an independent cohort including both drug-naïve and advanced Parkinson’s Disease patients, and healthy siblings of Parkinson’s Disease patients at higher genetic risk for developing the disease. While we did not find evidences of microRNAs co-regulated in Parkinson’s Disease and ageing, we report that hsa-miR-144-3p is consistently down-regulated in early Parkinson’s Disease patients. Moreover, interestingly, functional analysis revealed that hsa-miR-144-3p is involved in the regulation of coagulation, a process known to be altered in Parkinson’s Disease. Our results consistently show the down-regulation of hsa-mir144-3p in early Parkinson’s Disease, robustly confirmed across a variety of analytical and experimental analyses. These promising results ask for further research to unveil the functional details of the involvement of hsa-mir144-3p in Parkinson’s Disease.
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