| 1. |
- Kehoe, Laura, et al.
(författare)
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Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Baumann, Anne, et al.
(författare)
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HAMLET Forms Annular Oligomers When Deposited with Phospholipid Monolayers
- 2012
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 1089-8638 .- 0022-2836. ; 418:1-2, s. 90-102
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Tidskriftsartikel (refereegranskat)abstract
- Recently, the anticancer activity of human a-lactalbumin made lethal to tumor cells (HAMLET) has been linked to its increased membrane affinity in vitro, at neutral pH, and ability to cause leakage relative to the inactive native bovine alpha-lactalbumin (BLA) protein. In this study, atomic force microscopy resolved membrane distortions and annular oligomers (AOs) produced by HAMLET when deposited at neutral pH on mica together with a negatively charged lipid monolayer. BLA, BAMLET (HAMLET's bovine counterpart) and membrane-binding Peptide C, corresponding to BLA residues 75-100, also form AO-like structures under these conditions but at higher subphase concentrations than HAMLET. The N-terminal Peptide A, which binds to membranes at acidic but not at neutral pH, did not form AOs. This suggests a correlation between the capacity of the proteins/peptides to integrate into the membrane at neutral pH as observed by liposome content leakage and circular dichroism experiments and the formation of AOs, albeit at higher concentrations. Formation of AOs, which might be important to HAMLET's tumor toxic action, appears related to the increased tendency of the protein to populate intermediately folded states compared to the native protein, the formation of which is promoted by, but not uniquely dependent on, the oleic acid molecules associated with HAMLET. (C) 2012 Elsevier Ltd. All rights reserved.
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| 3. |
- Baumann, Anne, et al.
(författare)
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Tyrosine Hydroxylase Binding to Phospholipid Membranes Prompts Its Amyloid Aggregation and Compromises Bilayer Integrity
- 2016
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Tyrosine hydroxylase (TH), a rate-limiting enzyme in the synthesis of catecholamine neurotransmitters and hormones, binds to negatively charged phospholipid membranes. Binding to both large and giant unilamellar vesicles causes membrane permeabilization, as observed by efflux and influx of fluorescence dyes. Whereas the initial protein-membrane interaction involves the N-terminal tail that constitutes an extension of the regulatory ACT-domain, prolonged membrane binding induces misfolding and self-oligomerization of TH over time as shown by circular dichroism and Thioflavin T fluorescence. The gradual amyloid-like aggregation likely occurs through cross-beta interactions involving aggregation-prone motives in the catalytic domains, consistent with the formation of chain and ring-like protofilaments observed by atomic force microscopy in monolayer-bound TH. PC12 cells treated with the neurotoxin 6-hydroxydopamine displayed increased TH levels in the mitochondrial fraction, while incubation of isolated mitochondria with TH led to a decrease in the mitochondrial membrane potential. Furthermore, cell-substrate impedance and viability assays showed that supplementing the culture media with TH compromises cell viability over time. Our results revealed that the disruptive effect of TH on cell membranes may be a cytotoxic and pathogenic factor if the regulation and intracellular stability of TH is compromised.
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| 4. |
- Baumann, Henrikke, 1964, et al.
(författare)
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Det specifika med miljösystemanalysen
- 1999
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Sammanfattning av diskussion om vad forskning i ämnet miljösystemanalys innebär och innefattar.
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| 5. |
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| 6. |
- Baumann, Henrikke, 1964, et al.
(författare)
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LCA i ett nötskal
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)
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| 7. |
- Baumann, Henrikke, 1964, et al.
(författare)
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Switching the focus from product function to business profit: Introducing Business Model LCA (BM-LCA)
- 2022
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Ingår i: Proceedings of LCM 2021. - : EDP Sciences. ; 349
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Konferensbidrag (refereegranskat)abstract
- Recent years have seen much interest in business models as vehicles towards sustainability, cf. [1]. Conventional LCA, however, fails to properly capture the environmental impacts of a business model. Here, we introduce the background and the principles of Business Model LCA, a new LCA methodology for the assessment of environmental performance of business models. Methodological innovations are based on an understanding of the difference and relatedness between product and business. The key innovation is that BM-LCA centres its analysis on the ’business model’ instead of the ’product function’ as in conventional LCA. This requires the functional unit to represent the business (e.g., as ‘profit per time unit’) and the need to couple the monetary flows of the business to the material and energy flows of the product system via a set of ’coupling’ equations. BM-LCA contributes to environmental business analysis and could open up a new avenue of research where LCA and business analysts collaborate on business model innovation for sustainability.
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| 8. |
- Baumann, Henrikke, 1964, et al.
(författare)
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The Hitch Hiker's Guide to LCA. An orientation in life cycle assessment methodology and application
- 2004
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- The environmental life cycle of a product consists of all the stages from raw material extraction through production and use to waste management. Life cycle assessment (LCA), then, is the assessment of the environmental impact of a product throughout its life cycle. The holistic perspective that LCA provides on the environmental performance of products has made it a central concept for both environmental management in industry and environmental policy-making in public government. This is a textbook on LCA for those who want to learn the practice of LCA, e.g. environmental engineers, environmental managers and ecodesigners. The title paraphrases Douglas AdamsÕ famous story The Hitch HikerÕs Guide to the Galaxy, in which the machine Deep Thought after seven and a half million years of computing come up with Ô42Õ as the answer to the Ôgreat Question of Life, the Universe and EverythingÕ. Expectations on LCA are often similarÑsimple answer to difficult environmental dilemmas, and the result often as incomprehensible as Ô42Õ, unless one knows how to interpret LCA methodology and results.LCA applications are manifold. LCA can be used in e.g. product development, marketing, production and waste management. There are special requirements on LCA methodology for each type of application and the book presents a broad repertoire of methodological alternatives in LCA, their different implications for LCA results and their usefulness in different applications.The book is organised in three parts covering LCA methodology, LCA applications and exercises on LCA. Two introductory chapters give a general overview of the LCA concept and its historical development. After that, LCA methodology is described in detail in six chapters. Different fields of LCA application are covered in five subsequent chapters. Since the aim of the book is to teach the execution of LCA, there are also a number of exercises. Smaller exercises train different aspects of LCA methodology and prepare for the larger ones, ten complete LCA exercise projects.
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| 9. |
- Baumann, Henrikke, 1964, et al.
(författare)
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The Usefulness of an Actor’s Perspective in LCA
- 2011
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Ingår i: Towards Life Cycle Sustainability Management. - Dordrecht : Springer Netherlands. - 9789400718982 ; , s. 73-83
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Konferensbidrag (refereegranskat)abstract
- This paper is an argumentation for adding an actor’s perspective to lifecycleassessment (LCA). The need for this perspective stems from a criticismabout the usefulness of LCA interpretation methods comparing the relativecontribution of life-cycle phases of a product. Our argumentation is based on fourpreviously published studies providing practical examples of how value chainactors’ influence may be considered in an LCA and the benefit of doing so.Manufacturing sector examples show how one company's influence can beillustrated in results and how it may relate all relevant emissions to its ownprocesses. The food sector study shows how to assess several value chain actors’individual improvement potential. The final example, taken from building sector,explore how to consider the fact that actors in one part of the value chain caninfluence other actors to improve.
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| 10. |
- Breznau, Nate, et al.
(författare)
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Observing many researchers using the same data and hypothesis reveals a hidden universe of uncertainty
- 2022
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 119:44
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Tidskriftsartikel (refereegranskat)abstract
- This study explores how researchers analytical choices affect the reliability of scientific findings. Most discussions of reliability problems in science focus on systematic biases. We broaden the lens to emphasize the idiosyncrasy of conscious and unconscious decisions that researchers make during data analysis. We coordinated 161 researchers in 73 research teams and observed their research decisions as they used the same data to independently test the same prominent social science hypothesis: that greater immigration reduces support for social policies among the public. In this typical case of social science research, research teams reported both widely diverging numerical findings and substantive conclusions despite identical start conditions. Researchers expertise, prior beliefs, and expectations barely predict the wide variation in research outcomes. More than 95% of the total variance in numerical results remains unexplained even after qualitative coding of all identifiable decisions in each teams workflow. This reveals a universe of uncertainty that remains hidden when considering a single study in isolation. The idiosyncratic nature of how researchers results and conclusions varied is a previously underappreciated explanation for why many scientific hypotheses remain contested. These results call for greater epistemic humility and clarity in reporting scientific findings.
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