| 1. |
- Alén, Claes, 1949, et al.
(author)
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Lime/Cement Column Stabilised Soil - A New Model for settlement calculation
- 2005
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In: Deep Mixing '05.
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Conference paper (peer-reviewed)abstract
- A new model for calculation of settlements for an embankment on lime/cement stabilised clay is presented. The model can be regarded as a development of the current Swedish practice, which is based upon a simplified model, adopted in the computer program Limeset. The goal has been to incorporate recent years experience and research in a qualitative and realistic way. The model is based on the assumption that the deformation properties of the natural as well as the stabilised clay can be modelled in a conventional way. Hence, the development is to give a realistic description of the interaction between these two parts of the soil. A notable feature of this is to describe the stress distribution in the soil in a simple, yet relevant way.
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| 2. |
- Bernander, Stig, et al.
(author)
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Downhill progressive landslides in long natural slopes: triggering agents and landslide phases modeled with a finite difference method
- 2016
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In: Canadian geotechnical journal (Print). - : Canadian Science Publishing. - 0008-3674 .- 1208-6010. ; 53:10, s. 1565-1582
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Journal article (peer-reviewed)abstract
- A large landslide in Tuve (Gothenburg, Sweden 1977) initiated the development of a model for slope stability analysis taking the deformation-softening of soft sensitive clays into consideration. The model studies triggering agents and five phases in progressive slope failure are identified: (1) in-situ, (2) disturbance, (3) unstable ‘dynamic’, (4) transitory (or permanent) equilibrium, and (5) ‘global’ failure. The clay resistance in these phases may differ widely; mostly due to different rates of loading. Two time dependent failure criteria are defined: (i) the triggering load condition in the disturbance Phase (2), and (ii) the transitory equilibrium in Phase (4), indicating whether minor downhill displacements or a veritable landslide catastrophe will occur. The analysis explains why downhill landslides tend to spread over vast areas of almost horizontal ground further down-slope. The model has been applied to landslides in Scandinavia and Canada. Three case studies are briefly discussed. The model is a finite difference approach, where local downhill deformations caused by normal forces is maintained compatible with deviatory shear deformations above the potential (or the established) failure surface. Software and an easy-to-use spreadsheet are introduced as well as recent developments. See also Video Abstract.
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| 3. |
- Bonagas, Nadilly, et al.
(author)
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Pharmacological targeting of MTHFD2 suppresses acute myeloid leukemia by inducing thymidine depletion and replication stress
- 2022
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In: NATURE CANCER. - : Springer Science and Business Media LLC. - 2662-1347. ; 3:2, s. 156-
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Journal article (peer-reviewed)abstract
- The folate metabolism enzyme MTHFD2 (methylenetetrahydrofolate dehydrogenase/cyclohydrolase) is consistently overexpressed in cancer but its roles are not fully characterized, and current candidate inhibitors have limited potency for clinical development. In the present study, we demonstrate a role for MTHFD2 in DNA replication and genomic stability in cancer cells, and perform a drug screen to identify potent and selective nanomolar MTHFD2 inhibitors; protein cocrystal structures demonstrated binding to the active site of MTHFD2 and target engagement. MTHFD2 inhibitors reduced replication fork speed and induced replication stress followed by S-phase arrest and apoptosis of acute myeloid leukemia cells in vitro and in vivo, with a therapeutic window spanning four orders of magnitude compared with nontumorigenic cells. Mechanistically, MTHFD2 inhibitors prevented thymidine production leading to misincorporation of uracil into DNA and replication stress. Overall, these results demonstrate a functional link between MTHFD2-dependent cancer metabolism and replication stress that can be exploited therapeutically with this new class of inhibitors. Helleday and colleagues describe a nanomolar MTHFD2 inhibitor that causes replication stress and DNA damage accumulation in cancer cells via thymidine depletion, demonstrating a potential therapeutic strategy in AML tumors in vivo.
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| 4. |
- Casas, Joan Ramon, et al.
(author)
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Safety and Probabilistic Modelling : Sustainable Bridges Background document D4.4
- 2007
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Reports (peer-reviewed)abstract
- The main objective of this background document is to bridge the gap between the most advanced structural assessment techniques based on probabilistic methods and the daily practice of bridge evaluators in the railway agencies, not specifically trained on them and responsible of the load and resistance assessments. To this end, the background document explains and summarizes the basis of the safety assessment using a probabilistic approach, providing simplified methods whenever possible and also providing examples of application in order to make the documents more readable and understandable.The present background document is divided in the following deliverables:D4.4.1 Safety format and required safety levelsD4.4.2 Probabilistic modellingD4.4.3 Probabilistic non-linear analysisD4.4.4 Examination of fatigue safety and remaining fatigue life of structural details andcomponents in steel of railway bridges using probabilistic methodsD4.4.5 Long-term behaviour of subsoil below railway embankments – A simplified predictionmethod of settlements with a probabilistic approach
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| 5. |
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| 6. |
- Ma, Jianqin, et al.
(author)
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Apparent Earth Pressures of Soft Soils overlying Hard Bedrock at South Link in Stockholm
- 2007
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In: Proceedings of the 4th International Conference on Soft Soil Engineering - Soft Soil Engineering. - : Taylor & Francis Group. - 9780415422802 ; , s. 299-307
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Conference paper (peer-reviewed)abstract
- The apparent earth pressures measured at the South Link Project in Stockholm are used to analyze the pressure on sheet pile walls at deep excavations in soft soils overlying bedrock. The depths of the excavations vary from 3 m to 16 m and the toes of the walls are keyed into bedrock. Measured apparent earth pressures are over the Peck's (1969) envelope for soft clay but approximate to initial stress at rest. The normalized distribution pattern of the pressures indicates a large apparent earth pressure in the upper part of a test section. The well kept anchor load contributes to the large apparent earth pressure. This case indicates that the beneficial effect of the presence of bedrock may be over-shadowed by the low strength of soft soils, especially when the distance from excavation level to bedrock is larger than the depth of excavation.
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| 7. |
- Ma, Jianqin, et al.
(author)
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Back Analysis on a Deep Excavation in Stockholm with Finite Element Method
- 2006
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In: Proceedings of the 6th European Conference on Numerical Methods in Geotechnical Engineering - Numerical Methods in Geotechnical Engineering. - : Taylor & Francis Group. - 0415408229 ; , s. 423-429
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Conference paper (peer-reviewed)abstract
- A deep excavation section from the South Link in Stockholm is analyzed with 2D PLAXIS. The excavation, protected by anchor back-tied sheet pile walls, is 12-m-deep and 43-m-wide in soft soils overlying bedrock. Except wall installation, six soil excavations, five anchor preloading stages and seven consolidation analysis stages are presented in a Mohr-Coulomb model. The back-calculated lateral displacements in the numerical model are about 15% less than the monitored in the middle and lower parts of the test section, while about 20% larger in the upper part. The analysis results for the stage of soil excavation are closer to the monitoring results than for the stage of anchor preloading. The magnitude of settlement behind the sheet pile wall in the numerical model is less than that of the monitoring result. Negative soil volume strain is selectively used to discuss a possible mechanism with significant settlement increase.
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| 8. |
- Melbourne, Clive, et al.
(author)
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Masonry Arch Bridges : Sustainable Bridges Background document 4.7
- 2007
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Reports (peer-reviewed)abstract
- A recent survey showed that 40% of existing rail network bridges in Europe are masonry arches. More than 60% of those bridges are over 100 years old and still carry ever increasing levels of loading and increasing volumes of traffic. Due to the constantly increasing weight of rail traffic there is increasing demand for better understanding of their life expectancy and fatigue limits. It is imperative that the bridge stock is not adversely affected by these changes in the loading regime and that appropriate assessment, modelling, repair and strengthening techniques are available.This background document is an attempt to address and solve the above questions.To this end, the document is divided in the following parts:D4.7.1 Structural assessment of masonry arch bridgesD4.7.2 Numerical analyses of load distribution and deflections in railway bridge transitionzones due to passing trainsD4.7.3 Methods of analysis of damaged masonry arch bridgesD4.7.4 Potentiality of probabilistic methods in the assessment of masonry arches
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| 9. |
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| 10. |
- Wester, Kenneth, et al.
(author)
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Cultured human fibroblasts in agarose gel as a multi-functional control for immunohistochemistry : Standardization Of Ki67 (MIB1) assessment in routinely processed urinary bladder carcinoma tissue
- 2000
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In: Journal of Pathology. - 0022-3417 .- 1096-9896. ; 190:4, s. 503-11
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Journal article (peer-reviewed)abstract
- Immunohistochemistry (IHC) in clinical practice is hampered by lack of standardization and by subjectivity in interpretation and quantitation. This study aimed to develop a control system for IHC in routinely fixed and histoprocessed tissues. Such a system should be easy to handle in clinical practice and should reflect variations in fixation time, section thickness, section storage conditions, and staining protocols. In addition, in image analysis quantitation of immunostained tissues, when using classifiers computed on IHC-control images, the control system should be very stable. Cultured human fibroblasts were suspended in agarose, transferred into a length of tubing and stored at 4 degrees C. Three pieces of the cellgel control were separately fixed, histoprocessed, and paraffin-embedded as external controls. One piece was prepared together with each of 18 bladder carcinoma biopsies as internal controls. Slides with sections from the biopsy and all types of cellgel controls were stored at different temperatures and then stained using three different IHC protocols. The fibroblasts were homogeneously distributed in the agarose gel. Variation in section thickness did not influence immunostaining as evaluated by the MIB1 labelling index (MIB1 LI). The external controls decreased notably in MIB1 LI with increased fixation time. This was not seen in the 18 internal controls that were each fixed with a fresh biopsy. However, section storage and immunostaining conditions influenced the MIB1 expression equally in all control types and to a similar degree to the biopsies. Furthermore, colour-based image analysis quantitation of MIB1 LI in biopsies proved stable and independent of the control type used to compute the classifier.
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