| 1. |
- Sitch, S., et al.
(author)
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Recent trends and drivers of regional sources and sinks of carbon dioxide
- 2015
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In: Biogeosciences. - : Copernicus GmbH. - 1726-4189. ; 12:3, s. 653-679
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Journal article (peer-reviewed)abstract
- The land and ocean absorb on average just over half of the anthropogenic emissions of carbon dioxide (CO2) every year. These CO2 "sinks" are modulated by climate change and variability. Here we use a suite of nine dynamic global vegetation models (DGVMs) and four ocean biogeochemical general circulation models (OBGCMs) to estimate trends driven by global and regional climate and atmospheric CO2 in land and oceanic CO2 exchanges with the atmosphere over the period 1990-2009, to attribute these trends to underlying processes in the models, and to quantify the uncertainty and level of inter-model agreement. The models were forced with reconstructed climate fields and observed global atmospheric CO2; land use and land cover changes are not included for the DGVMs. Over the period 1990-2009, the DGVMs simulate a mean global land carbon sink of -2.4 +/- 0.7 PgC yr(-1) with a small significant trend of -0.06 +/- 0.03 PgC yr(-2) (increasing sink). Over the more limited period 1990-2004, the ocean models simulate a mean ocean sink of -2.2 +/- 0.2 PgC yr(-1) with a trend in the net C uptake that is indistinguishable from zero (-0.01 +/- 0.02 PgC yr(-2)). The two ocean models that extended the simulations until 2009 suggest a slightly stronger, but still small, trend of 0.02 +/- 0.01 PgC yr(-2). Trends from land and ocean models compare favourably to the land greenness trends from remote sensing, atmospheric inversion results, and the residual land sink required to close the global carbon budget. Trends in the land sink are driven by increasing net primary production (NPP), whose statistically significant trend of 0.22 +/- 0.08 PgC yr(-2) exceeds a significant trend in heterotrophic respiration of 0.16 +/- 0.05 PgC yr(-2) - primarily as a consequence of widespread CO2 fertilisation of plant production. Most of the land-based trend in simulated net carbon uptake originates from natural ecosystems in the tropics (0.04 +/- 0.01 PgC yr(-2)), with almost no trend over the northern land region, where recent warming and reduced rainfall offsets the positive impact of elevated atmospheric CO2 and changes in growing season length on carbon storage. The small uptake trend in the ocean models emerges because climate variability and change, and in particular increasing sea surface temperatures, tend to counteract the trend in ocean uptake driven by the increase in atmospheric CO2. Large uncertainty remains in the magnitude and sign of modelled carbon trends in several regions, as well as regarding the influence of land use and land cover changes on regional trends.
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| 2. |
- Sitch, S., et al.
(author)
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Trends and drivers of regional sources and sinks of carbon dioxide over the past two decades
- 2013
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In: Biogeosciences Discussions. - : Copernicus GmbH. - 1810-6277. ; 10, s. 20113-20177
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Journal article (other academic/artistic)abstract
- Abstract. The land and ocean absorb on average over half of the anthropogenic emissions of carbon dioxide (CO2) every year. These CO2 "sinks" are modulated by climate change and variability. Here we use a suite of nine Dynamic Global Vegetation Models (DGVMs) and four Ocean Biogeochemical General Circulation Models (OBGCMs) to quantify the global and regional climate and atmospheric CO2 – driven trends in land and oceanic CO2 exchanges with the atmosphere over the period 1990–2009, attribute these trends to underlying processes, and quantify the uncertainty and level of model agreement. The models were forced with reconstructed climate fields and observed global atmospheric CO2; Land Use and Land Cover Changes are not included for the DGVMs. Over the period 1990–2009, the DGVMs simulate a mean global land carbon sink of −2.4 ± 0.7 Pg C yr−1 with a small significant trend of −0.06 ± 0.03 Pg C yr−2 (increasing sink). Over the more limited period 1990–2004, the ocean models simulate a mean ocean sink of –2.2 ± 0.2 Pg C yr–1 with a trend in the net C uptake that is indistinguishable from zero (−0.01 ± 0.02 Pg C yr−2). The two ocean models that extended the simulations until 2009 suggest a slightly stronger, but still small trend of −0.02 ± 0.01 Pg C yr−2. Trends from land and ocean models compare favourably to the land greenness trends from remote sensing, atmospheric inversion results, and the residual land sink required to close the global carbon budget. Trends in the land sink are driven by increasing net primary production (NPP) whose statistically significant trend of 0.22 ± 0.08 Pg C yr−2 exceeds a significant trend in heterotrophic respiration of 0.16 ± 0.05 Pg C yr−2 – primarily as a consequence of wide-spread CO2 fertilisation of plant production. Most of the land-based trend in simulated net carbon uptake originates from natural ecosystems in the tropics (−0.04 ± 0.01 Pg C yr−2), with almost no trend over the northern land region, where recent warming and reduced rainfall offsets the positive impact of elevated atmospheric CO2 on carbon storage. The small uptake trend in the ocean models emerges because climate variability and change, and in particular increasing sea surface temperatures, tend to counteract the trend in ocean uptake driven by the increase in atmospheric CO2. Large uncertainty remains in the magnitude and sign of modelled carbon trends in several regions, and on the influence of land use and land cover changes on regional trends.
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| 3. |
- Beal, Jacob, et al.
(author)
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Robust estimation of bacterial cell count from optical density
- 2020
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In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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