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  • Cassimjee, Karim Engelmark, et al. (author)
  • Streamlined Preparation of Immobilized Candida antarctica Lipase B
  • 2017
  • In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 2:12, s. 8674-8677
  • Journal article (peer-reviewed)abstract
    • Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
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