| 1. |
- Comin, Cesar H., et al.
(author)
-
Quantifying the regularity of a 3D set of points on the surface of an ellipsoidal object
- 2020
-
In: Pattern Recognition Letters. - : Elsevier BV. - 0167-8655. ; 133, s. 1-7
-
Journal article (peer-reviewed)abstract
- Several natural and artificial structures, such as human skin and mammals cortices, exhibit a compound organization, with basic elements being distributed along a surface. The problem of quantifying the geometrical uniformity of this type of biological and physical compound structures is addressed in this work. This required the solution of several problems, including the detection, along the surface, of the borders of the compound system, defining the adjacency between the elements in the 3D space, and obtaining a reference of uniformity for calculating the polygonality. Specific approaches were devised and applied to address each of these difficulties, including connectivity criteria ensuring the adjacency to remain within the considered surface as well as the extension of the polygonality, originally suggested for 2D structures, to 3D compound systems. The potential of the so-obtained method is illustrated with respect to compound eyes of fungus gnats (small, forest dwelling flies), and interesting results are reported and discussed, including the fact that the uniformity tends to increase toward the center of the system, and the absence of correlation with two measurements traditionally used for characterizing this type of eyes.
|
|
| 2. |
- Kur, Esther, et al.
(author)
-
Temporal modulation of collective cell behavior controls vascular network topology
- 2016
-
In: eLIFE. - : eLife Sciences Publications Ltd. - 2050-084X. ; 5
-
Journal article (peer-reviewed)abstract
- Vascular network density determines the amount of oxygen and nutrients delivered to host tissues, but how the vast diversity of densities is generated is unknown. Reiterations of endothelial-tip-cell selection, sprout extension and anastomosis are the basis for vascular network generation, a process governed by VEGF/Notch feedback loop. Here, we find that temporal regulation of this feedback loop, a previously unexplored dimension, is the key mechanism to determine vascular density. Iterating between computational modeling and in vivo live imaging, we demonstrate that the rate of tip-cell selection determines the length of linear sprout extension at the expense of branching, dictating network density. We provide the first example of a host tissue-derived signal (Semaphorin3E-Plexin-D1) that accelerates tip cell selection rate, yielding a dense network. We propose that temporal regulation of this critical, iterative aspect of network formation could be a general mechanism, and additional temporal regulators may exist to sculpt vascular topology.
|
|
