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  • MacLeod, Rebecca, et al. (author)
  • Selective isolation of hyaluronan by solid phase adsorption to silica
  • 2022
  • In: Analytical Biochemistry. - : Academic Press. - 0003-2697 .- 1096-0309. ; 652
  • Journal article (peer-reviewed)abstract
    • A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.
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