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- Eguíluz-Gracia, Ibon, et al.
(author)
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Long-Term persistence of human donor alveolar macrophages in lung transplant recipients
- 2016
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In: Thorax. - : BMJ. - 0040-6376 .- 1468-3296. ; 71:11, s. 1006-1011
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Journal article (peer-reviewed)abstract
- Background Alveolar macrophages (AMFs) are critical regulators of lung function, and may participate in graft rejection following lung transplantation. Recent studies in experimental animals suggest that most AMFs are self-maintaining cells of embryonic origin, but knowledge about the ontogeny and life span of human AMFs is scarce. Methods To follow the origin and longevity of AMFs in patients with lung transplantation for more than 100â €..weeks, we obtained transbronchial biopsies from 10 gender-mismatched patients with lung transplantation. These were subjected to combined in situ hybridisation for X/Y chromosomes and immunofluorescence staining for macrophage markers. Moreover, development of AMFs in humanised mice reconstituted with CD34+ umbilical cord-derived cells was assessed. Results The number of donor-derived AMFs was unchanged during the 2â €..year post-Transplantation period. A fraction of the AMFs proliferated locally, demonstrating that at least a subset of human AMFs have the capacity to self-renew. Lungs of humanised mice were found to abundantly contain populations of human AMFs expressing markers compatible with a monocyte origin. Moreover, in patients with lung transplantation we found that recipient monocytes seeded the alveoli early after transplantation, and showed subsequent phenotypical changes consistent with differentiation into proliferating mature AMFs. This resulted in a stable mixed chimerism between donor and recipient AMFs throughout the 2-year period. Conclusions The finding that human AMFs are maintained in the lung parenchyma for several years indicates that pulmonary macrophage transplantation can be a feasible therapeutic option for patients with diseases caused by dysfunctional AMFs. Moreover, in a lung transplantation setting, long-Term persistence of donor AMFs may be important for the development of chronic graft rejection.
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| 2. |
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Multi-faced Social Transformations : Challenges and Studies
- 2015
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Editorial proceedings (peer-reviewed)abstract
- Multi-faced Social Transformations: Challenges and Studies brings together the proceedings of the 7th Slovenian Social Science Conference, “The Challenges of Social Transformations”, held in September 2014. It was organized by the School of Advanced Social Studies (SASS), the Slovenian National Committee of the Management of Social Transformations programme (MOST), and the Slovenian National Commission for UNESCO. The multidisciplinary contributions presented here analyse various aspects of the economic, social, and cultural transformations that accompany the contemporary globalized world.The book consists of four sections dealing with particular areas of transformations. These include a range of political, economic and cultural dimensions that are observed from the macro-level, in social systems and structural changes, to the micro-level, in aspects of individuals’ lives. The book will be of interest for academics in the field of social sciences, as well as for civil society activists and policy makers. The frames of the transformations are not limited to the European space, and provide a more global perspective.
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| 3. |
- Weinstein, John N., et al.
(author)
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The cancer genome atlas pan-cancer analysis project
- 2013
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In: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:10, s. 1113-1120
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Research review (peer-reviewed)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
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