| 1. |
- Bannak Gedara, Shishanthi Jayarathna, et al.
(author)
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High fructan barley lines produced by selective breeding may alter beta-glucan and amylopectin molecular structure
- 2023
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In: Carbohydrate Polymers. - 0144-8617. ; 316
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Journal article (peer-reviewed)abstract
- Six cross-bred barley lines developed by a breeding strategy with the target to enhance the fructan synthesis activity and reduce the fructan hydrolysis activity were analyzed together with their parental lines, and a reference line (Gustav) to determine whether the breeding strategy also affected the content and molecular structure of amylopectin and beta-glucan. The highest fructan and beta-glucan content achieved in the novel barley lines was 8.6 % and 12 %, respectively (12.3-fold and 3.2-fold higher than in Gustav). The lines with low fructan synthesis activity had higher starch content, smaller building blocks in amylopectin, and smaller structural units of beta-glucans than the lines with high-fructan synthesis activity. Correlation analysis confirmed that low starch content was associated with high amylose, fructan, and beta-glucan content, and larger building blocks in amylopectin.
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| 2. |
- Dotsenko, Gleb, et al.
(author)
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Material disintegration affects enzymatic determination of beta-glucan in barley and oats
- 2019
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In: Journal of Cereal Science. - : Elsevier BV. - 0733-5210 .- 1095-9963. ; 88, s. 138-144
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Journal article (peer-reviewed)abstract
- Material disintegration is the primary step in beta-glucan determination in cereal grains. This study investigated the effect of disintegration approach on beta-glucan content, determined by an enzymatic method in mature kernels of barley and oat. Milling with a coffee grinder was found to be relevant only for pre-disintegration and homogenization of the grain (4.6% and 3.2% beta-glucan content was found in barley and oats, respectively). Ball milling appeared to be the most efficient approach for reducing material particle size, but resulted in samples with underestimated beta-glucan content (4.6% and 3.3% in barley and oats, respectively). Grinding with mortar and pestle in liquid nitrogen and repeated centrifugal milling were found to be the optimal disintegration methods for barley kernels (5.2% and 4.7-4.8% beta-glucan content, respectively). Grinding with mortar and pestle (either in dry state or in liquid nitrogen) and single centrifugal milling were the optimal disintegration methods for oat kernels (3.6-3.9% and 3.3-3.4% beta-glucan content, respectively).
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| 3. |
- Fei, Mingliang, et al.
(author)
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Achieving of high-diet-fiber barley via managing fructan hydrolysis
- 2022
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 12
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Journal article (peer-reviewed)abstract
- High fructan content in the grain of cereals is an important trait in agriculture such as environmental resilience and dietary fiber food production. To understand the mechanism in determining final grain fructan content and achieve high fructan cereal, a cross breeding strategy based on fructan synthesis and hydrolysis activities was set up and have achieved barley lines with 11.8% storage fructan in the harvested grain. Our study discovered that high activity of fructan hydrolysis at later grain developmental stage leads to the low fructan content in mature seeds, simultaneously increasing fructan synthesis at early stage and decreasing fructan hydrolysis at later stage through crossing breeding is an efficient way to elevate grain diet-fiber content. A good correlation between fructan and beta glucans was also discovered with obvious interest. Field trials showed that the achieved high fructan barley produced over seven folds higher fructan content than control barley and pull carbon-flux to fructan through decreasing fructan hydrolysis without disruption starch synthesis will probably not bring yield deficiency.
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