| 1. |
- Bowers, Robert M., et al.
(author)
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Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
- 2017
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In: Nature Biotechnology. - : NATURE PUBLISHING GROUP. - 1087-0156 .- 1546-1696. ; 35:8, s. 725-731
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Journal article (peer-reviewed)abstract
- We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.
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| 3. |
- Blombach, Fabian, et al.
(author)
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Archaeal MBF1 binds to 30S and 70S ribosomes via its helix-turn-helix domain
- 2014
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In: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 462, s. 373-384
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Journal article (peer-reviewed)abstract
- MBF1 (multi-protein bridging factor 1) is a protein containing a conserved HTH (helix-turn-helix) domain in both eukaryotes and archaea. Eukaryotic MBF1 has been reported to function as a transcriptional co-activator that physically bridges transcription regulators with the core transcription initiation machinery of RNA polymerase II. In addition, MBF1 has been found to be associated with polyadenylated mRNA in yeast as well as in mammalian cells. aMBF1 (archaeal MBF1) is very well conserved among most archaeal lineages; however, its function has so far remained elusive. To address this, we have conducted a molecular characterization of this aMBF1. Affinity purification of interacting proteins indicates that aMBF1 binds to ribosomal subunits. On sucrose density gradients, aMBF1 co-fractionates with free 30S ribosomal subunits as well as with 70S ribosomes engaged in translation. Binding of aMBF1 to ribosomes does not inhibit translation. Using NMR spectroscopy, we show that aMBF1 contains a long intrinsically disordered linker connecting the predicted N-terminal zinc-ribbon domain with the C-terminal HTH domain. The HTH domain, which is conserved in all archaeal and eukaryotic MBF1 homologues, is directly involved in the association of aMBF1 with ribosomes. The disordered linker of the ribosome-bound aMBF1 provides the N-terminal domain with high flexibility in the aMBF1 ribosome complex. Overall, our findings suggest a role for aMBF1 in the archaeal translation process.
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| 4. |
- Dellaert, B. G. C., et al.
(author)
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Multi-faceted tourist travel decisions : A constraint-based conceptual framework to describe tourists' sequential choices of travel components
- 2012
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In: Tourism Management. - : Taylor and Francis. - 9781136002823 - 0080435890 - 9780080435893 ; , s. 9-20
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Book chapter (other academic/artistic)abstract
- Assume that tourists' travel choices can be regarded as the outcome of a sequential scheduling process in which a number of decisions are scheduled over a longer period (e.g. varying from a few days to several months) and that those decisions take into account restrictions because of time and money budgets and coupling constraints caused by work, family and friendship-based relationships. Then, the specific contribution of this paper is to introduce a conceptual framework that allows one to analyze tourists' travel behavior that incorporates this type of temporal sequencing of multi-faceted travel decisions as well as relevant decision-making constraints. In line with most research on travel choice, our discussion will be restricted to holiday travel only. It should also be noted that though our analysis allows us to look at multiple facets within the trip choice, we focus on tourists' choices of single trips. Thus, we leave the extension of the proposed framework to include choices of multiple trips over longer periods of time for future research.
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| 5. |
- Eriksson, Lars, et al.
(author)
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Experimental simulation of car users’ switching to public transport
- 2010
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In: Transportation Letters. ; 2:3, s. 145-155
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Journal article (peer-reviewed)abstract
- In order to investigate how public transport should be improved to appear attractive to car users, this study develop and test an internet-based experimental simulation of travel mode choice dependent on the complexity of daily activity agendas. The results for 75 students taking part in the experimental simulation are compared to the results of a survey of another 69 students taking part in a pilot study. Students in both groups were frequent car users. The results of the survey showed that higher costs of car use and greater perceived difficulty to use public transport decreased intention to use the bus for the commute to the university. In the experimental simulation high access to bus stops increased bus use and reduced car use but less for a busy daily activity agenda when the car-use costs are low. The results highlight the importance of focusing on tradeoffs between increasing car-use costs and improvement of public transport.
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| 6. |
- Ettema, D, et al.
(author)
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Out-of-Home Activities, Daily Travel, and Subjective Well-Being
- 2010
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In: Transportation Research Part A. - : Elsevier. - 0965-8564 .- 1879-2375. ; 44, s. 723-732
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Journal article (peer-reviewed)abstract
- It is argued that utility theory that underpins current cost-benefit analyses of daily travel needs to be complemented. An alternative theoretical framework is to this end proposed which applies subjective well-being (SWB) to travel behaviour analysis. It is posited in this theoretical framework that participation in goal-directed activities, facilitated or hindered by travel, contributes to SWB, that the degree of travel-related stress in participating in these activities reduces SWB, and that positive affect associated with travel in itself has an impact on SWB.
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