| 1. |
- Berger, Cecilia, et al.
(author)
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Molecular characterization of PRM-associated endometrial changes, PAEC, following mifepristone treatment
- 2018
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In: Contraception. - : Elsevier. - 0010-7824 .- 1879-0518. ; 98:4, s. 317-322
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Journal article (peer-reviewed)abstract
- Objective: The progesterone receptor modulator (PRM) mifepristone holds the potential to be developed for regular contraception. However, long-term treatment can cause thickening of the endometrium and PRM-associated endometrial changes (PAEC). The objective of this study was to explore the molecular expression of endometrium displaying PAEC after mifepristone treatment in order to understand the future implications of PAEC and safety of long-term use. Study design: Endometrial biopsies were obtained from premenopausal women following 3 months of continuous mifepristone treatment. The biopsies were evaluated regarding occurrence of PAEC and followed up by a comparative analysis of gene expression in PAEC endometrium (n=7) with endometrium not displaying PAEC (n=4). Methods used included microarray analysis, Ingenuity Pathway Analysis (IPA) and real-time polymerase chain reaction. Results: Three genes relevant within endometrial function were up-regulated with PAEC: THY1 (p=.02), ADAM12 (p=.04) and TN-C (p=.04). The proliferation marker MKi67 was not altered (p=.31). None of the differentially regulated genes were involved in the endometrial cancer-signaling pathway (based on IPA knowledge database). Conclusion: The genes altered in endometrium displaying PAEC after 3 months of mifepristone exposure are mainly involved in the structural architecture of tissue. Implications: PAEC features may be explained by the altered genes and their networks affecting tissue architecture although not involved in endometrial cancer signaling pathways, and thus, treatment with mifepristone at this dosage does not show any adverse effect at endometrial level.
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| 2. |
- Bergqvist, Cecilia, et al.
(author)
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Multiplex Nucleic Acid Suspension Bead Arrays for Detection and Subtyping of Filoviruses
- 2015
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In: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 53:4, s. 1368-1370
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Journal article (peer-reviewed)abstract
- Here we describe multiplex suspension bead array systems that allow fast and reliable detection of reverse transcriptase (RT) PCR amplified filovirus genomes and also enable subtyping of Ebola virus species and Marburg virus strains. These systems have an analytical sensitivity equivalent to that of RT-PCR.
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| 3. |
- Bylin, Maria, 1974-
(author)
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Aspektuella hjälpverb i svenskan
- 2013
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Doctoral thesis (other academic/artistic)abstract
- This thesis tells the tales of auxiliation in Swedish. It describes how the auxiliary features of the aspectual auxiliaries bruka ‘use to’, tendera ‘tend to’, börja ‘begin, start’, fortsätta ‘continue’, sluta ‘stop, cease’ och hota ‘threaten’ evolve, as seen in a text corpora covering the 11th to the 21st century.A grammaticalization perspective is employed, which not unexpectedly has implications for the way data is interpreted and presented, but also less expectedly for how the method is understood and applied. The method consists of Swedish auxiliary tests, designed to reveal auxiliary features. The discussion of their validity involves auxiliation theory as well as a more general theoretical discussion of how the category and the function of auxiliaries have been conceived. The main point made is that a valid test should have an explicit link to auxiliary function. Establishing the nature of such a link, however, is no trivial matter and so the plausibility of the link turns out to be the main factor in judging the validity of auxiliary tests.In the early stages of the auxiliation processes, action nominals were often used as complements of the verbs under study. Later on these verbs started taking infinitive complements, lost their semantic restrictions on their subjects and started occurring in a type of passive construction where the nonfinite verb carries the semantic relationship to the arguments of the clause. The occurrence in this type of passive construction is perceived as the most plausible indication of auxiliary function available for subject raising auxiliaries, since the auxiliary and the nonfinite verb in these clauses do function together as one single predicate.The evolution of these aspectual auxiliaries essentially follows the predictions made by auxiliation theory. That said, they all show individual deviations from those predictions, depending on such factors as their semantics, their source construction and during which period in the history of Swedish their auxiliation took place.
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| 4. |
- Delsing, Lars-Olof, et al.
(author)
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Den stora katastrofen med för- och efterskalv : om kasussammanfallet i fornsvenska
- 2014
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In: Studier i svensk språkhistoria 12.
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Conference paper (peer-reviewed)abstract
- I denna uppsats gör jag två saker. För det första ger jag en kortfattad beskrivning över kasussammanfallet i fornsvenska. För det andra diskuterar jag orsakerna till kasussammanfallet. Jag identifierar två viktiga tidsperioder då kasussystemet förändras. Den första inträffar kring 1300 och den andra kring 1450, åtminstone i Mälardalen. Jag kallar den första för Lilla katastrofen och den andra för Stora katastrofen. Jag kommer att visa att Lilla katastrofen innebär en förenkling. Framför allt förenklas tilldelningen av kasus på predikatens argument, men förenklingen sker även i nominalfrasen. Kasusanvändningen blir i stor utsträckning redundant. Jag visar också att Stora katastrofen innebär ett nästan fullständigt bortfall av alla kasusformer (utom på personliga pronomen). Jag undersöker också Stora katastrofens orsaker, och kan visa att den sker tidigast i Stockholmsområdet och Kalmar, och jag hävdar att den därmed främst bör sättas i samband med det stora inslaget av lågtyskar i dessa städer.
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| 5. |
- Dillner, Joakim, et al.
(author)
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Antibodies to SARS-CoV-2 and risk of past or future sick leave
- 2021
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In: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
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Journal article (peer-reviewed)abstract
- The extent that antibodies to SARS-CoV-2 may protect against future virus-associated disease is unknown. We invited all employees (n=15,300) at work at the Karolinska University Hospital, Stockholm, Sweden to participate in a study examining SARS-Cov-2 antibodies in relation to registered sick leave. For consenting 12,928 healthy hospital employees antibodies to SARS-CoV-2 could be determined and compared to participant sick leave records. Subjects with viral serum antibodies were not at excess risk for future sick leave (adjusted odds ratio (OR) controlling for age and sex: 0.85 [95% confidence interval (CI) (0.85 (0.43-1.68)]. By contrast, subjects with antibodies had an excess risk for sick leave in the weeks prior to testing [adjusted OR in multivariate analysis: 3.34 (2.98-3.74)]. Thus, presence of viral antibodies marks past disease and protection against excess risk of future disease. Knowledge of whether exposed subjects have had disease in the past or are at risk for future disease is essential for planning of control measures.Trial registration: First registered on 02/06/20, ClinicalTrials.gov NCT04411576.
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| 6. |
- Edfeldt, Chatarina, 1963-, et al.
(author)
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Northern Crossings : Translation, Circulation and the Literary Semi-periphery
- 2022
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Book (peer-reviewed)abstract
- This open access book uses Swedish literature and the Swedish publishing field as recurring examples to describe and analyse the role of the literary semi-peripheral position in world literature from various perspectives and on meso, micro and macro levels, using both quantitative and qualitative methods. This includes the role of translation in the semi-periphery and the conditions under which literature travels to and from that position. The focus is not on Sweden, as such, but rather on the semi-peripheral transitional space as exemplified by the Swedish case.Consisting of three co-written chapters, this study sheds light on what might be called the semi-peripheral condition or the semi-periphery as an area of transition. As part of the Cosmopolitan and Vernacular Dynamics in World Literatures series, it makes continuous use of the concepts of 'cosmopolitan' and 'vernacular' – or rather, the processual terms, cosmopolitanization and vernacularization – which provide an overall structure to the analysis of literature and literary phenomena. In this way, the authors show that the semi-periphery is an ideal point of departure to further the understanding of world literature, because it is a place where the cosmopolitan (the literary universal) and the vernacular (the rootedness in a particular culture or place) interact in ways that have not yet been thoroughly explored.
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| 7. |
- Fagman, Johan Bourghardt, 1980, et al.
(author)
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EGFR, but not COX-2, protein in resected pancreatic ductal adenocarcinoma is associated with poor survival.
- 2019
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In: Oncology letters. - : Spandidos Publications. - 1792-1074 .- 1792-1082. ; 17:6, s. 5361-5368
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Journal article (peer-reviewed)abstract
- The effects of EGFR and COX-2 protein overexpression on clinical outcomes in pancreatic ductal adenocarcinoma (PDAC) patients remains unclear. Therefore, the aim of the present study was to evaluate the protein expression of epithelial growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) in tumor cells in surgically resected PDAC, in comparison with clinicopathological characteristics and clinical outcomes. Immunohistochemical staining of formalin-fixed paraffin-embedded tissue derived from surgically resected tumors was performed. Tissue slides were evaluated for membrane wild-type EGFR and cytoplasmic COX-2 staining using a histoscore system. Statistical associations between EGFR and COX-2 staining and clinicopathological characteristics were examined to predict survival. In a cohort of 32 resected PDAC patients, high EGFR protein expression in tumor cells was significantly associated with shorter median overall survival (7.9 vs. 39.2 months, P=0.0038). The corresponding hazard ratio (HR) for patients with high EGFR protein expression in tumor cells was 3.12 [95% confidence interval (CI): 1.39-7.00, P=0.006]. COX-2 protein expression was not associated with survival (22.6 vs. 24.5 months P=0.60; HR 1.22 95% CI: 0.59-2.51, P=0.60). Following multivariate Cox regression analysis, high EGFR protein expression in tumor cells (P=0.043) remained as significant independent prognostic factor for survival. In conclusion, high wild-type EGFR protein expression, but not COX-2 protein expression, in tumor cells is a prognostic factor for reduced overall survival following pancreatic tumor resection, supporting a role for EGFR in identifying resected patients that may benefit from EGFR-targeted therapy.
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| 8. |
- Falk, A, et al.
(author)
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Gene delivery to adult neural stem cells
- 2002
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In: Experimental Cell Research. - : Elsevier BV. - 1090-2422 .- 0014-4827. ; 279:1, s. 34-39
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Journal article (peer-reviewed)abstract
- Neural stem cells may present an ideal route for gene therapy as well as offer new possibilities for the replacement of neurons lost to injury or disease. However, it has proved difficult to express ectopic genes in stem cells. We report methods to introduce genes into adult neural stem cells using viral and nonviral vectors in vitro and in vivo. Adenoviral and VSV-G-pseudotyped retroviral vectors are more efficient than plasmid transfection or VSV-G lentiviral transduction in vitro. We further show that adult neural stem cells can be directed to a neuronal fate by ectopic expression of neurogenin 2 in vitro. Plasmids can be delivered in vivo when complexed with linear polyethyleneimine, and gene expression can be targeted specifically to neural stem or progenitor cells by the use of specific promoters. These techniques may be utilized both to study the function of various genes in the differentiation of neural stem cells to specific cell fates and, ultimately, for gene therapy or to generate specific differentiated progeny for cell transplantation. (C) 2002 Elsevier Science (USA).
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| 9. |
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| 10. |
- Falk, Cecilia, et al.
(author)
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Introduction
- 2003
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In: Nordic Journal of Linguistics. - 0332-5865. ; 26:2, s. 127-131
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Journal article (peer-reviewed)
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