1. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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2. |
- Chow, Joe. H., et al.
(author)
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Preliminary synchronized phasor data analysis of disturbance events in the US Eastern Interconnection
- 2009
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In: Power Systems Conference and Exposition, 2009. PSCE ’09. IEEE/PES. - : IEEE. - 9781424438105 ; , s. 136-143
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Conference paper (peer-reviewed)abstract
- This paper presents analysis results of synchronized phasor data from 10 disturbance events recorded in the US Eastern Interconnection (EI). The phasor data covers a wide region in the EI, allowing for the study of disturbance propagation, interarea modes, and oscillations in voltages and currents. The analysis is not straightforward because the EI is a meshed system with adequate interarea mode damping. Disturbances involving tripping a single large generator unit produce very short interarea swing responses. Islanding events involving regions at the perimeter, however, provide more prominent responses for analysis.
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3. |
- Fan, Hongqi, et al.
(author)
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2D Spatial Keystone Transform for Sub-Pixel Motion Extraction from Noisy Occupancy Grid Map
- 2018
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In: Proceedings of 21st International Conference on Information Fusion (FUSION). - 9780996452762 - 9781538643303 ; , s. 2400-2406
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Conference paper (peer-reviewed)abstract
- In this paper, we propose a novel sub-pixel motion extraction method, called as Two Dimensional Spatial Keystone Transform (2DS-KST), for the motion detection and estimation from successive noisy Occupancy Grid Maps (OGMs). It extends the KST in radar imaging or motion compensation to 2D real spatial case, based on multiple hypotheses about possible directions of moving obstacles. Simulation results show that 2DS-KST has a good performance on the extraction of sub-pixel motions in very noisy environment, especially for those slowly moving obstacles.
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4. |
- Felsberg, Michael, 1974-, et al.
(author)
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The Thermal Infrared Visual Object Tracking VOT-TIR2016 Challenge Results
- 2016
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In: Computer Vision – ECCV 2016 Workshops. ECCV 2016.. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 824-849
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Conference paper (peer-reviewed)abstract
- The Thermal Infrared Visual Object Tracking challenge 2016, VOT-TIR2016, aims at comparing short-term single-object visual trackers that work on thermal infrared (TIR) sequences and do not apply pre-learned models of object appearance. VOT-TIR2016 is the second benchmark on short-term tracking in TIR sequences. Results of 24 trackers are presented. For each participating tracker, a short description is provided in the appendix. The VOT-TIR2016 challenge is similar to the 2015 challenge, the main difference is the introduction of new, more difficult sequences into the dataset. Furthermore, VOT-TIR2016 evaluation adopted the improvements regarding overlap calculation in VOT2016. Compared to VOT-TIR2015, a significant general improvement of results has been observed, which partly compensate for the more difficult sequences. The dataset, the evaluation kit, as well as the results are publicly available at the challenge website.
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5. |
- Kristan, Matej, et al.
(author)
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The Visual Object Tracking VOT2015 challenge results
- 2015
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In: Proceedings 2015 IEEE International Conference on Computer Vision Workshops ICCVW 2015. - : IEEE. - 9780769557205 ; , s. 564-586
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Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge 2015, VOT2015, aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 62 trackers are presented. The number of tested trackers makes VOT 2015 the largest benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the appendix. Features of the VOT2015 challenge that go beyond its VOT2014 predecessor are: (i) a new VOT2015 dataset twice as large as in VOT2014 with full annotation of targets by rotated bounding boxes and per-frame attribute, (ii) extensions of the VOT2014 evaluation methodology by introduction of a new performance measure. The dataset, the evaluation kit as well as the results are publicly available at the challenge website(1).
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6. |
- Kristan, Matej, et al.
(author)
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The Visual Object Tracking VOT2016 Challenge Results
- 2016
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In: COMPUTER VISION - ECCV 2016 WORKSHOPS, PT II. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 777-823
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Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published at major computer vision conferences and journals in the recent years. The number of tested state-of-the-art trackers makes the VOT 2016 the largest and most challenging benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the Appendix. The VOT2016 goes beyond its predecessors by (i) introducing a new semi-automatic ground truth bounding box annotation methodology and (ii) extending the evaluation system with the no-reset experiment.
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7. |
- Kristan, Matej, et al.
(author)
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The Visual Object Tracking VOT2017 challenge results
- 2017
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In: 2017 IEEE INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2017). - : IEEE. - 9781538610343 ; , s. 1949-1972
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Conference paper (peer-reviewed)abstract
- The Visual Object Tracking challenge VOT2017 is the fifth annual tracker benchmarking activity organized by the VOT initiative. Results of 51 trackers are presented; many are state-of-the-art published at major computer vision conferences or journals in recent years. The evaluation included the standard VOT and other popular methodologies and a new "real-time" experiment simulating a situation where a tracker processes images as if provided by a continuously running sensor. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The VOT2017 goes beyond its predecessors by (i) improving the VOT public dataset and introducing a separate VOT2017 sequestered dataset, (ii) introducing a realtime tracking experiment and (iii) releasing a redesigned toolkit that supports complex experiments. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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8. |
- Wu, Yitong, et al.
(author)
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Numerical and experiment study on ventilation performance of the equipment compartment of Alpine high-speed train
- 2023
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In: Engineering Applications of Computational Fluid Mechanics. - 1994-2060 .- 1997-003X. ; 17:1
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Journal article (peer-reviewed)abstract
- Alpine High-Speed Train serves on the Lanzhou-Urumqi Line in northwest China, where the terrain is mainly the Gobi Desert. To adapt to this complex environment, the independent-air duct is mainly used for the electrical facilities inside the equipment compartment to prevent the spread of sand particles. This isolated air duct makes ventilation characteristics of the equipment susceptible to the external environment. For this reason, this work aims to clarify and investigate the ventilation characteristic of electrical facilities. A two-step simulation method using IDDES (Improved Delayed Detached Eddy Simulation) and a real-vehicle tracking test using the T-typed pitot tubes were conducted. In the simulation, it is found that the ventilation performance can be influenced by the location of the equipment compartment, facilities and the fan mounted inside. By comparing the results of the test and simulation, they share the same characteristic that the air outlet volume of the converter near the head car is being promoted while it near the tail car is being inhibited. The maximum deviation ratio between the test and simulation is 9%. Therefore, the measurement method in this study is relatively reliable.
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