| 1. |
- Kröger, A, et al.
(author)
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The severity of human peri-implantitis lesions correlates with the level of submucosal microbial sysbiosis
- 2018
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In: Journal of Clinical Periodontology. - 0303-6979 .- 1600-051X. ; 45:12, s. 1498-1509
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Journal article (peer-reviewed)abstract
- AIM: To cross-sectionally analyze the submucosal microbiome of peri-implantitis (PI) lesions at different severity levels.MATERIALS AND METHODS: Microbial signatures of 45 submucosal plaque samples from untreated peri-implantitis lesions obtained from 30 non-smoking, systemically healthy subjects were assessed by 16s sequencing. Linear mixed models were used to identify taxa with differential abundance by probing depth, after correction for age, gender, and multiple samples per subject. Network analyses were performed to identify groups of taxa with mutual occurrence or exclusion. Subsequently, the effects of peri-implant probing depth on submucosal microbial dysbiosis was calculated using the microbial dysbiosis index.RESULTS: In total, we identified 337 different taxa in the submucosal microbiome of peri-implantitis. Total abundance of 12 taxa correlated significantly with increasing probing depth; a significant relationship with lower probing depth was found for 16 taxa. Network analysis identified two mutually exclusive complexes associated with shallow pockets and deeper pockets, respectively. Deeper peri-implant pockets were associated with significantly increased dysbiosis.CONCLUSION: Increases in peri-implant pocket depth are associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis. This article is protected by copyright. All rights reserved.
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| 2. |
- Kröger, A, et al.
(author)
-
The severity of human peri-implantitis lesions correlates with the level of submucosal microbial sysbiosis
- 2018
-
In: Journal of Clinical Periodontology. - : Blackwell Munksgaard. - 0303-6979 .- 1600-051X. ; 45:12, s. 1498-1509
-
Journal article (peer-reviewed)abstract
- AIM: To cross-sectionally analyze the submucosal microbiome of peri-implantitis (PI) lesions at different severity levels. MATERIALS AND METHODS: Microbial signatures of 45 submucosal plaque samples from untreated peri-implantitis lesions obtained from 30 non-smoking, systemically healthy subjects were assessed by 16s sequencing. Linear mixed models were used to identify taxa with differential abundance by probing depth, after correction for age, gender, and multiple samples per subject. Network analyses were performed to identify groups of taxa with mutual occurrence or exclusion. Subsequently, the effects of peri-implant probing depth on submucosal microbial dysbiosis was calculated using the microbial dysbiosis index. RESULTS: In total, we identified 337 different taxa in the submucosal microbiome of peri-implantitis. Total abundance of 12 taxa correlated significantly with increasing probing depth; a significant relationship with lower probing depth was found for 16 taxa. Network analysis identified twomutually exclusive complexes associated with shallow pockets and deeper pockets, respectively. Deeper peri-implant pockets were associated with significantly increased dysbiosis. CONCLUSION: Increases in peri-implant pocket depth are associated with substantial changes in the submucosal microbiome and increasing levels of dysbiosis. This article is protected by copyright. All rights reserved.
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| 3. |
- Thoma, Daniel S., et al.
(author)
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Soft tissue management at implants: : Summary and consensus statements of group 2. The 6th EAO Consensus Conference 2021
- 2021
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In: Clinical Oral Implants Research. - : John Wiley & Sons. - 0905-7161 .- 1600-0501. ; 32:S21, s. 174-180
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Journal article (peer-reviewed)abstract
- Objectives: The task of working Group 2 at the 6th Consensus Meeting of the European Association for Osseointegration was to comprehensively assess the effects of soft tissue augmentation procedures at dental implant sites on clinical, radiographic and patient-reported outcome measures (PROMs) including an overview on available outcome measures and methods of assessment.Materials and methods: Three systematic reviews and one critical review were performed in advance on (i) the effects of soft tissue augmentation procedures on clinical, radiographic and aesthetic outcomes, (ii) reliability and validity of outcome measures and methods of assessment and (iii) PROMs applied in clinical studies for soft tissue augmentation procedures at dental implant sites. Major findings, consensus statements, clinical recommendations and implications for future research were discussed in the group and approved during the plenary sessions.Results: The four reviews predominantly revealed: Soft tissue augmentation procedures in conjunction with immediate and delayed implant placement result in superior aesthetic outcomes compared to no soft tissue augmentation in the zone of aesthetic priority. Soft tissue augmentation procedures have a limited effect on marginal bone level changes compared to implant sites without soft tissue augmentation. Clinically relevant parameters (gingival index, mucosal recession) and plaque control improve at implant sites when the width of keratinised mucosa is increased. A variety of aesthetic indices have been described with good reliability. Pink Esthetic Score and Complex Esthetic Index are the most validated aesthetic indices for single implants, though. Superimposed digital surface scans are most accurate to assess profilometric tissue changes. PROMs following soft tissue augmentation procedures have been assessed using various forms of questionnaires. Soft tissue augmentation had a limited effect on PROMs.Conclusions: Soft tissue augmentation procedures are widely applied in conjunction with implant therapy. Depending on the indication of these interventions, clinical, radiographic and aesthetic outcomes may improve, whereas the effect on PROMs is limited.
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| 4. |
- Fickl, S., et al.
(author)
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Porcine dermal matrix in the treatment of dehiscence-type defects - an experimental split-mouth animal trial
- 2015
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In: Clinical Oral Implants Research. - : Wiley. - 0905-7161. ; 26:7, s. 799-805
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Journal article (peer-reviewed)abstract
- Objectives: To describe histometrical outcomes (tissue thickness, tissue height) of a porcine dermal matrix (PDX) and subepithelial connective tissue (CTG) in the treatment of dehiscence-type defects. Material and methods: In five beagle dogs buccal dehiscence defects were created on both upper canines. The defects were covered in a split-mouth design either with a porcine dermal matrix or subepithelial connective tissue. After 4months histometrical outcomes were evaluated using a nonparametric Brunner-Langer model. Results: Neither in the test nor in the control specimen signs of inflammation or foreign body reaction was detected. Histometrically, no significant difference was found for tissue thickness and height between both treatment groups. Conclusions: Porcine dermal matrix can be used for grafting of dehiscence-type defects. Augmentation of tissue thickness seems to be comparable to subepithelial connective tissue.
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| 5. |
- Fischer, K, et al.
(author)
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Influence of local administration of pamidronate on extraction socket healing : a histomorphometric proof-of-principle preclinical in vivo evaluation
- 2014
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In: Clinical Oral Implants Research. - : John Wiley & Sons. - 0905-7161 .- 1600-0501. ; 25:Suppl 10, s. 148-148
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Journal article (other academic/artistic)abstract
- Background: Tooth extraction is conventionally performed without any additional tissue manipulation and left for healing by secondary intention. Maintaining hard and soft tissue volume after tooth extraction may be crucial to achieve a highly aesthetic restoration and it may also facilitate stability of the results on the long-term. Therefor, different approaches have been proposed to eliminate post-extraction ridge reduction. Today, however, no technique seems to be able to preserve the entire socket volume. Aim/Hypothesis: To evaluate histomorphometrically the influence of local administration of pamidronate, adsorbed on a deproteinized porcine bone substitute, on extraction socket healing. Material and methods: Two American Fox-hound dogs were used within this proof-of-principle study. Following endodontic treatment of the distal root of the three lower premolars, the teeth were hemisected and the mesial roots were extracted flapless. The sockets were then loosely filled, in a split-mouth fashion, with a deproteinized porcine bone substitute in particulate form (Osteobiol Gen-Os; DPB), rehydrated either with sterile saline (control) or 90 mg/ml pamidronic acid solution (Aredia ; test). Extraction sockets were sealed with connective tissue punches obtained from the palate and secured with sutures. After 4 months of healing, specimens containing the sockets sites and remaining roots were retrieved and histomorphometrically evaluated. Vertical and horizontal ridge changes were evaluated comparing the section containing the remaining root and the corresponding extraction sites. Results: Histological evaluation of the sections revealed significant differences in healing patterns between test and controls. While the latter group presented complete closure of the sockets with newly formed bone, pamidronate treated sites presented with open socket entrances, only sealed with soft connective tissue. Within the socket, control sites presented with various amounts of newly formed bone and no evidence of DPB; limited amounts of bone healing were observed within test sites, that were filled with DPB mainly embedded in connective tissue. Socket (bone) wall loss in a vertical dimension showed only minor differences between tests and controls sites (buccal: – 1.01 vs – 1.15 mm; lingual: – 0.92 vs – 1.15 mm;). Horizontal bone loss, measured at a level corresponding to 3 mm below the cemento-enamel-junction (CEJ), was nearly three times higher in control sites ( 2.19 1.81 mm vs. – 0.80 0.91 mm;), while no differences were observed between groups at a level corresponding to 5 mm below the CEJ. Conclusion and clinical implications: Local administration of pamidronate adsorbed on a deproteinized porcine bone substitute in particulate form appeared to delay extraction socket healing, but may also reduce post-extraction dimensional changes in the alveolar ridge, in terms of horizontal bone loss. Additionally, pamidronate appears to obstruct resorption of the porcine bone substitute.
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| 6. |
- Schubert, Jasmin S., et al.
(author)
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Nature of the Active Ni State for Photocatalytic Hydrogen Generation
- 2023
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In: Advanced Materials Interfaces. - : WILEY. - 2196-7350.
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Journal article (peer-reviewed)abstract
- Thermal treatments can have detrimental effects on the photocatalytic hydrogen (H2) evolution performance and impact the formation mechanism of the active state of surface-supported co-catalysts. In this work, a range of Ni-based co-catalysts is investigated immobilized on TiO2, evaluated their H2 evolution rates in situ over 21 h, and analyzed the samples at various stages with a comprehensive set of spectroscopic and microscopy techniques. It is found that achieving the optimal hydrogen evolution (HER) performance requires the right Ni0:Ni2+ ratio, rather than only Ni0, and that Ni needs to be weakly adsorbed on the TiO2 surface to create a dynamic state. Under these conditions, Ni can undergo an efficient redox shuttle, involving the transformation of Ni2+ to Ni0 and back after releasing the accumulated electrons for H+ reduction (i.e., Ni2+ <-> Ni0). Yet, when the calcination temperature of the Ni/TiO2 photocatalysts increases, resulting in stronger coordination/adsorption of Ni on TiO2, this process is gradually inhibited, which ultimately leads to decreased HER performances. This work emphasizes the significance and influence of thermal treatments on the Ni active state formation - a process that can be relevant to other HER co-catalysts. This research underscores the impact of thermal treatment on the formation of Nis active state for hydrogen evolution reaction (HER) . For optimal performance, Ni should weakly adsorb onto the substrate, efficiently shuttling between Ni2+ and Ni0 and reversing after H+ reduction (Ni2+ <-> Ni0). However, raising the calcination temperature strengthens Ni coordination/adsorption on the substrate, gradually inhibiting this process and reducing HER performances.image
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