| 1. |
- Formosa-Jordan, Pau, et al.
(author)
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Single-Cell Approaches for Understanding Morphogenesis Using Computational Morphodynamics
- 2018
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In: Mathematical Modelling in Plant Biology. - Cham : Springer International Publishing. - 9783319990699 - 9783319990705 ; , s. 87-106
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Book chapter (other academic/artistic)abstract
- In multicellular organisms cells grow, divide and adopt different fates, resulting in tissues and organs with specific functions. In recent years, a number of studies have brought quantitative knowledge about how these processes are orchestrated, shedding new light on cells as active and central players in morphogenesis. We explore recent advances in understanding plant morphogenesis from a quantitative perspective, defining the research field of Computational Morphodynamics. The focus is on studies combining theoretical and experimental approaches integrating hypotheses of how molecular and mechanical regulation at the cellular level lead to tissue behaviour. Finally, we discuss some of the main challenges for future work.
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| 2. |
- Landrein, Benoit, et al.
(author)
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Nitrate modulates stem cell dynamics in Arabidopsis shoot meristems through cytokinins
- 2018
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 115:6, s. 1382-1387
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Journal article (peer-reviewed)abstract
- The shoot apical meristem (SAM) is responsible for the generation of all the aerial parts of plants. Given its critical role, dynamical changes in SAM activity should play a central role in the adaptation of plant architecture to the environment. Using quantitative microscopy, grafting experiments, and genetic perturbations, we connect the plant environment to the SAM by describing the molecular mechanism by which cytokinins signal the level of nutrient availability to the SAM. We show that a systemic signal of cytokinin precursors mediates the adaptation of SAM size and organogenesis rate to the availability of mineral nutrients by modulating the expression of WUSCHEL, a key regulator of stem cell homeostasis. In time-lapse experiments, we further show that this mechanism allows meristems to adapt to rapid changes in nitrate concentration, and thereby modulate their rate of organ production to the availability of mineral nutrients within a few days. Our work sheds light on the role of the stem cell regulatory network by showing that it not only maintains meristem homeostasis but also allows plants to adapt to rapid changes in the environment.
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| 3. |
- Meyer, Heather M, et al.
(author)
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Fluctuations of the transcription factor ATML1 generate the pattern of giant cells in the Arabidopsis sepal
- 2017
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In: eLife. - 2050-084X.
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Journal article (peer-reviewed)abstract
- Multicellular development produces patterns of specialized cell types. Yet, it is often unclear how individual cells within a field of identical cells initiate the patterning process. Using live imaging, quantitative image analyses and modeling, we show that during Arabidopsis thaliana sepal development, fluctuations in the concentration of the transcription factor ATML1 pattern a field of identical epidermal cells to differentiate into giant cells interspersed between smaller cells. We find that ATML1 is expressed in all epidermal cells. However, its level fluctuates in each of these cells. If ATML1 levels surpass a threshold during the G2 phase of the cell cycle, the cell will likely enter a state of endoreduplication and become giant. Otherwise, the cell divides. Our results demonstrate a fluctuation-driven patterning mechanism for how cell fate decisions can be initiated through a random yet tightly regulated process.
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