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1.	Krause, A, et al. (author) Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver 2003 In: Protein science : a publication of the Protein Society. - : Wiley. - 0961-8368. ; 12:1, s. 143-152 Journal article (peer-reviewed)
2.	Machtejevas, E, et al. (author) Automated multi-dimensional liquid chromatography: sample preparation and identification of peptides from human blood filtrate 2004 In: Journal of Chromatography. B. - : Elsevier BV. - 1873-376X .- 1570-0232. ; 803:1, s. 121-130 Journal article (peer-reviewed)abstract A comprehensive on-line sample clean-up with an integrated two-dimensional HPLC system was developed for the analysis of natural peptides. Samples comprised of endogenous peptides with molecular weights up to 20 kDa were generated from human hemofiltrate (HF) obtained from patients with chronic renal failure. The (poly-)peptides were separated using novel silica-based restricted access materials with strong cation-exchange functionalities (SCX-RAM). The size-selective sample fractionation step is followed by cation-exchange chromatography as the first dimension. The subsequent second dimension of separation is based on hydrophobic interaction using four parallel short reversed-phase (RP) columns implemented via a fully automated column switching technique. More than 1000 peaks were resolved within the total analysis time of 96 min. Substances of selected peaks were sampled to analyse their molecular weights by off-line MALDI-TOF mass spectrometry and to determine their amino acid sequence by Edman degradation. The potential for comprehensive peptide mapping and identification is demonstrated.
3.	STANDKER, L, et al. (author) In vivo degradation of human fibrinogen A alpha: detection of cleavage sites and release of antithrombotic peptides 1995 In: Biochemical and biophysical research communications. - : Elsevier BV. - 0006-291X. ; 215:3, s. 896-902 Journal article (peer-reviewed)

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Type of publication: journal article (3)

Type of content: peer-reviewed (3)

Author/Editor: Forssmann, WG (3); Sillard, R (2); Standker, L (2); Krause, A. (1); Marko-Varga, György (1); Raida, M (1); show more...; Wagner, K. (1); John, H (1); Adermann, K (1); Conejo-Garcia, JR (1); Kleemeier, B (1); Kluver, E (1); Maronde, E (1); Schulz-Knappe, P (1); Nehls, MC (1); Wattler, F (1); Wattler, S (1); Machtejevas, E (1); Bischoff, R (1); Unger, KK (1); BENSCH, KW (1); RUF, A (1); SCHULZKNAPPE, P (1); SCHEPKY, AG (1); PATSCHEKE, H (1); show less...

University: Karolinska Institutet (2); Lund University (1)

Language: English (3)

Research subject (UKÄ/SCB): Natural sciences (1)

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