| 1. |
- Wayne, Greg, et al.
(author)
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Principles of Systems Biology, No. 11
- 2016
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In: CELL SYSTEMS. - : CELL PRESS. - 2405-4712. ; 3:5, s. 406-410
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Journal article (peer-reviewed)abstract
- This month: AI that learns patterns and facts, new protein-RNA and protein-protein relationships, engineering signaling and metabolism, and more variants of Cas9.
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| 2. |
- Dahlström, Märta, et al.
(author)
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Development of a fluorescent intensity assay amenable for high-throughput screening for determining 15-lipoxygenase activity.
- 2010
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In: Journal of Biomolecular Screening. - : Elsevier BV. - 1087-0571 .- 1552-454X. ; 15:6, s. 671-9
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Journal article (peer-reviewed)abstract
- 15-Lipoxygenase-1 catalyzes the introduction of molecular oxygen into polyunsaturated fatty acids to form a lipid hydroperoxide. The authors have developed an assay for the detection of lipid hydroperoxides formed by human 15-lipoxygenase (15-LO) in enzyme or cellular assays using either a 96-well or a 384-well format. The assays described take advantage of the ability of lipid hydroperoxides to oxidize nonfluorescent diphenyl-1-pyrenylphosphine (DPPP) to a fluorescent phosphine oxide. Oxidation of DPPP yields a fluorescent compound, which is not sensitive to temperature and is stable for more than 2 h. The assay is sensitive toward inhibition and robust with a Z' value of 0.79 and 0.4 in a 96- and 384-well format, respectively, and thus amenable for high-throughput screening. The utility of DPPP as a marker for 15-lipoxygenase activity was demonstrated with both enzyme- and cell-based assays for the identification of hits and to determine potency by IC(50) determinations.
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| 3. |
- Edqvist, Per-Henrik D., et al.
(author)
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Loss of ASRGL1 expression is an independent biomarker for disease-specific survival in endometrioid endometrial carcinoma
- 2015
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In: Gynecologic Oncology. - : Elsevier BV. - 0090-8258 .- 1095-6859. ; 137:3, s. 529-537
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Journal article (peer-reviewed)abstract
- Objective For endometrial carcinoma, prognostic stratification methods do not satisfactorily identify patients with adverse outcome. Currently, histology, tumor grade and stage are used to tailoring surgical treatment and to determine the need for adjuvant treatment. Low-risk patients are not considered to require adjuvant therapy or staging lymphadenectomy. For patients with intermediate or high risk, some guidelines recommend tailoring adjuvant treatment according to additional negative prognostic factors. Our objective was to evaluate the biomarker potential of the ASRGL1 protein in endometrial carcinoma. Methods Using The Human Protein Atlas (www.proteinatlas.org), the l-asparaginase (ASRGL1) protein was identified as an endometrial carcinoma biomarker candidate. ASRGL1 expression was immunohistochemically evaluated with an extensively validated antibody on two independent endometrial carcinoma cohorts (n = 229 and n = 286) arranged as tissue microarrays. Staining results were correlated with clinical features. Results Reduced expression of ASRGL1, defined as < 75% positively stained tumor cells, was significantly associated with poor prognosis and reduced disease-specific survival in endometrioid endometrial adenocarcinoma (EEA). In multivariate analysis the hazard ratios for disease-specific survival were 3.55 (95% CI = 1.10-11.43; p = 0.003) and 3.23 (95% CI = 1.53-6.81; p = 0.002) in the two cohorts, respectively. Of the 48 cases with Grade 3 Stage I tumor all disease-related deaths were associated with low ASRGL1 expression. Conclusions Loss of ASRGL1 in EEA is a powerful biomarker for poor prognosis and retained ASRGL1 has a positive impact on survival. ASRGL1 immunohistochemistry has potential to become an additional tool for prognostication in cases where tailoring adjuvant treatment according to additional prognostic factors besides grade and stage is recommended.
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| 4. |
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| 5. |
- Forsström, Bjorn, et al.
(author)
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Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays
- 2014
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In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 13:6, s. 1585-1597
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Journal article (peer-reviewed)abstract
- Antibodies are of importance for the field of proteomics, both as reagents for imaging cells, tissues, and organs and as capturing agents for affinity enrichment in mass-spectrometry-based techniques. It is important to gain basic insights regarding the binding sites (epitopes) of antibodies and potential cross-reactivity to nontarget proteins. Knowledge about an antibody's linear epitopes is also useful in, for instance, developing assays involving the capture of peptides obtained from trypsin cleavage of samples prior to mass spectrometry analysis. Here, we describe, for the first time, the design and use of peptide arrays covering all human proteins for the analysis of antibody specificity, based on parallel in situ photolithic synthesis of a total of 2.1 million overlapping peptides. This has allowed analysis of on-and off-target binding of both monoclonal and polyclonal antibodies, complemented with precise mapping of epitopes based on full amino acid substitution scans. The analysis suggests that linear epitopes are relatively short, confined to five to seven residues, resulting in apparent off-target binding to peptides corresponding to a large number of unrelated human proteins. However, subsequent analysis using recombinant proteins suggests that these linear epitopes have a strict conformational component, thus giving us new insights regarding how antibodies bind to their antigens.
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| 6. |
- Forsström, David, et al.
(author)
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Group versus Internet-based cognitive-behavioral therapy for procrastination : Study protocol for a randomized controlled trial
- 2015
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In: Abstracts from the 7th Swedish Congress on internet interventions (SWEsrii). - Linköping : Linköping University Press. ; , s. 20-20
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Conference paper (peer-reviewed)abstract
- Procrastination can be defined as a voluntarily delay of an intended course of action despite expecting to be worse-off for the delay, and is considered a persistent behavior pattern that can result in major psychological suffering, which in turn can constitute a major problem for many individuals. There a not many studies available on treating procrastination. A previous Internet based RCT with guided and unguided treatment aimed to decrease procrastination yielded positive results for individuals who volunteered to receive treatment. This current study has the aim to further increase the knowledge regarding treatment for procrastination. It is a RCT comparing unguided and group treatment aimed to decrease procrastination in a student population. Demographic data from the included participants and preliminary outcome data is presented. These results are discussed and compared to the previous RCT and other studies evaluating the effect of treatment aimed to decrease procrastination.
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| 7. |
- Forsström, David, 1981-, et al.
(author)
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Measuring loot box consumption and negative consequences : Psychometric investigation of a Swedish version of the Risky Loot Box Index
- 2022
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In: Addictive Behaviors Reports. - : Elsevier BV. - 2352-8532. ; 16
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Journal article (peer-reviewed)abstract
- Loot boxes are products in videogames that is earned by playing a video game or by buying them. Loot boxes has similar mechanisms as a lottery and there is an ongoing debate if loot boxes are gambling. However, to understand the potential harm of loot boxes valid instruments are needed. An English study psychometrically evaluated an instrument focused on risky loot box use called the Risky Loot Box Index. The study evaluated 12 item scale and based on a factor analysis it was reduced to a five-item scale. The aim of our study is to evaluate a Swedish version of the 12-item instrument from a psychometric perspective. Two samples recruited via an online survey were used. The first sample was recruited from the gambling site Unibet. A mail with an invitation to participate was sent to esports bettors and sport bettors at the gambling site. The second sample was recruited from a Facebook forum focused on e-sports. An invitation was posted on the forum to partake in the study. A total of 195 of respondents (96% men and 4% women) with a mean age of 33.76 (SD = 12.34) answered the Unibet survey and 169 respondents (96% men and 4% women and non-binary) with a mean age of 23.89 (SD = 5.52) answered the Facebook survey. An exploratory factor analysis yielded a two-factor seven item solution. The factors were overconsumption regarding time spent on loot boxes and the other factor was focused on overconsumption of loot boxes in terms of spending money. The confirmatory factor analysis conducted in the Facebook sample validated the result from the exploratory factor analysis. The conclusion of the study is that the Swedish version of the Risky Loot Index has good psychometric properties and can used to measure risky loot box consumption.
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| 8. |
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| 9. |
- Forsström, Stefan, 1984-, et al.
(author)
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Specialanpassade kurser för yrkesverksamma ingenjörer : Erfarenheter och upplevelser
- 2023
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In: Bidrag från den 9:e utvecklingskonferensen för Sveriges ingenjörsutbildningar. - : Mälardalens universitet. - 9789174856200 ; , s. 348-353
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Conference paper (other academic/artistic)abstract
- I dagens samhälle blir det allt viktigare att fortbilda sig under hela sitt yrkesverksamma liv. För att möta efterfrågan på det livslånga lärandet har Mittuniversitetet utvecklat och genomfört ett antal kurser som riktar sig mot yrkesverksamma ingenjörer. Detta arbete presenterar våra erfarenheter av att ge dessa kurser, med en tyngdpunkt på studenternas upplevelser. Syftet med detta är att bygga upp en vetenskaplig bas för vad vi gör som är bra, men även vad som kan förbättras och förändras. Målsättningen är att göra dessa specialanpassade kurser riktade mot yrkesverksamma ingenjörer så givande och flexibla som möjligt. Våra initiala resultat visar bland annat att studenternas negativa upplevelser ofta var kopplade till antagningsförfarandet och det praktiska genomförandet av kurserna. Man hade svårigheter med att hitta hur man skulle registrera sig på kursen och att tidsramen för registrering kunde vara ett problem. Läroplattformen uppfattades som svår att överblicka och det förekom även viss otydlighet gällande var undervisningen skulle äga rum. Den positiva responsen i utvärderingarna gällde främst det faktiska kursinnehållet, då man ansåg att uppgifter och kursmaterial var givande. Vidare uppskattades kursupplägget, att man kunde kombinera studierna med arbete. Framledes kommer vi att fortsätta med dessa utvärderingar i takt med att kurserna ges, och därefter anpassa vårt mottagande och kommunikationen med studenterna. Även kursupplägget ses över kontinuerligt via den återkoppling vi mottar.
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| 10. |
- Hermanson, Mark H., et al.
(author)
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Deposition History of Brominated Flame Retardant Compounds in an Ice Core from Holtedahlfonna, Svalbard, Norway
- 2010
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In: Environmental Science and Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 44:19, s. 7405-7410
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Journal article (peer-reviewed)abstract
- Brominated flame retardants (BFRs) have been found in Arctic wildlife, lake sediment, and air. To identify the atmospheric BFR deposition history on Svalbard, Norway, we analyzed 19 BFRs, including hexabromocyclododecane (HBCD), 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), decabromodiphenyl ethane (DBDPE), pentabromoethylbenzene (PBEB), and 15 polybrominated diphenyl ether congeners (PBDE) in the upper 34 m of an ice core (representing 1953-2005) from Holtedahlfonna, the western-most ice sheet on Svalbard. All of the non-PBDE compounds were detected in nearly continuous profiles in the core. Seven PBDEs were not observed above background (28, 47, 66, 100, 99, 154, 153), while 4 were found in 1 or 2 of 6 segments (17, 85, 138, 183). BDEs-49, 71, 190, 209 had nearly continuous profiles but only BDE-209 in large amounts. The greatest inputs were HBCD and BDE-209, 910, and 320 pg cm(-2) yr(-1) from 1995-2005. DBDPE, BTBPE, and PBEB show nearly continuous input growth in recent core segments, but all were <6 pg cm(-2) yr(-1). Long-range atmospheric processes may have moved these particle-bound BFRs to the site, probably during the Arctic haze season. Average air mass trajectories over 10 years show >75% of atmospheric flow to Holtedahlfonna coming from Eurasia during haze periods (March and April).
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