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Träfflista för sökning "WFRF:(Ganan Calvo Alfonso M.) "

Search: WFRF:(Ganan Calvo Alfonso M.)

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1.
  • Wiedorn, Max O., et al. (author)
  • Megahertz serial crystallography
  • 2018
  • In: Nature Communications. - : Nature Publishing Group. - 2041-1723. ; 9
  • Journal article (peer-reviewed)abstract
    • The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
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2.
  • Mühlig, Kerstin, et al. (author)
  • Nanometre-sized droplets from a gas dynamic virtual nozzle
  • 2019
  • In: Journal of Applied Crystallography. - : INT UNION CRYSTALLOGRAPHY. - 1600-5767 .- 0021-8898. ; 52, s. 800-808
  • Journal article (peer-reviewed)abstract
    • This paper reports on improved techniques to create and characterize nanometre-sized droplets from dilute aqueous solutions by using a gas dynamic virtual nozzle (GDVN). It describes a method to measure the size distribution of uncharged droplets, using an environmental scanning electron microscope, and provides theoretical models for the droplet sizes created. The results show that droplet sizes can be tuned by adjusting the gas and liquid flow rates in the GDVN, and at the lowest liquid flow rates, the size of the water droplets peaks at about 120 nm. This droplet size is similar to droplet sizes produced by electrospray ionization but requires neither electrolytes nor charging of the solution. The results presented here identify a new operational regime for GDVNs and show that predictable droplet sizes, comparable to those obtained by electrospray ionization, can be produced by purely mechanical means in GDVNs.
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3.
  • Yefanov, Oleksandr, et al. (author)
  • Evaluation of serial crystallographic structure determination within megahertz pulse trains
  • 2019
  • In: Structural Dynamics. - : AMER INST PHYSICS. - 2329-7778. ; 6:6
  • Journal article (peer-reviewed)abstract
    • The new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure determination. An outstanding but crucial question is whether the use of a pulse repetition rate nearly four orders of magnitude higher than previously possible results in unwanted structural changes due to either radiation damage or systematic effects on data quality. Here, separate structures from the first and subsequent pulses in the European XFEL pulse train were determined, showing that there is essentially no difference between structures determined from different pulses under currently available operating conditions at the European XFEL.
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