| 1. |
- Larsson, Dhana E., et al.
(author)
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Identification and evaluation of potential anti-cancer drugs on human neuroendocrine tumor cell lines
- 2006
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In: Anticancer Research. - 0250-7005 .- 1791-7530. ; 26:6B, s. 4125-4129
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Journal article (peer-reviewed)abstract
- The aim of this study was to investigate drug sensitivity in neuroendocrine tumor cell lines. Materials and Methods: In vitro drug sensitivity screening was performed using the fluorometric microculture cytotoxicity assay in one human pancreatic carcinoid and two human bronchial carcinoid cell lines. In addition, a normal human retinal pigment epithelial cell line was used for comparison. A total of 18 drugs with different mechanisms of action were tested. Results: The most active agents were brefeldin A, emetine, bortezomib and idarubicin, having IC50 values < 1 μM in all four cell lines. In addition, the three tumor cell lines showed sensitivity for sanguinarine, Bay11-7085, mitoxantrone, doxorubicin, β-lapachone, NSC 95397 and CGP-74514A. Conclusion: The cell lines were sensitive for several drugs acting in different ways, covering a broad spectrum of mechanisms of action. Some of these compounds may possibly be used in clinical trials and show therapeutic effect in patients with neuroendocrine tumors.
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| 2. |
- Larsson, Pär, et al.
(author)
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Canonical insertion-deletion markers for rapid DNA typing of Francisella tularensis
- 2007
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In: Emerging Infectious Diseases. - 1080-6040 .- 1080-6059. ; 13:11, s. 1725-1732
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Journal article (peer-reviewed)abstract
- To develop effective and accurate typing of strains of Francisella tularensis, a potent human pathogen and a putative bioterrorist agent, we combined analysis of insertion-deletion (indel) markers with multiple-locus variable-number tandem repeat analysis (MLVA). From 5 representative F. tularensis genome sequences, 38 indel markers with canonical properties, i.e., capable of sorting strains into major genetic groups, were selected. To avoid markers with a propensity for homoplasy, we used only those indels with 2 allelic variants and devoid of substantial sequence repeats. MLVA included sequences with much diversity in copy number of tandem repeats. The combined procedure allowed subspecies division, delineation of clades A.I and A.II of subspecies tularensis, differentiation of Japanese strains from other strains of subspecies holarctica, and high-resolution strain typing. The procedure uses limited amounts of killed bacterial preparations and, because only 1 single analytic method is needed, is time- and cost-effective.
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| 3. |
- Lindh, Magnus, 1960-
(author)
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Regionen och EU? : Uppfattningar och attityder till EU-relaterade frågor i Västsverige
- 2016
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Doctoral thesis (other academic/artistic)abstract
- This thesis explores perceptions and preferences on regional action in EU-related frameworks among regional actors in Western Sweden. Building upon the literature on Europeanisation and the Fusion approach, three dimensions of Europeanisation are clarified and explored– download, upload and crossload – and together with a set of five variables that constitute the Micro Fusion Framework; a comprehensive analytical tool is developed. The thesis analyses the intense debate among the members of West Sweden that took place from 2011 to 2013 that focused on how to functionally organise the regional office in Brussels in order to meet future challenges. Surprisingly, the members eventually decided to terminate their cooperation and close the jointly owned office in Brussels in spite of the fact that it has been widely regarded as successful and effective. Diverging perceptions and preferences is understood in terms of three positions on regional action; a download-, upload- and a coherent oriented position. Finally, the thesis presents the empirical findings and discusses in relation to three fusion scenarios, infusion, defusion and clustered fusion.In terms of Micro Fusion Framework, the dynamics shaping why West Sweden was finally regarded as a dysfunctional arena for regional action are explained by a shift of attention and action among regional actors in Western Sweden that led to pressure for further institutional adaptation in order to meet the demand of how ‘to get the best out of the EU’. Further, this redefinition of how to handle EU-affairs within the upload-oriented position was accompanied by positive attitudes towards the potential to bypass the state and thereby pursue regional priorities directly in Brussels given the compound nature of the EU. In contrast, those regional actors that are found to be more download-oriented often question the benefits of uploading activities in practice and advocate close relations to the state. A coherent oriented position recognises the importance of activities related to both of the vertical dimensions of Europeanisation.
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| 4. |
- Olovsson, Tord Göran, 1967-, et al.
(author)
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På väg mot kollektivt lärande – inom ramen för samverkan mellan skola och universitet
- 2024
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In: Forskning och utveckling i förskola och skola (Fufos). - Piteå : Utbildningsförvaltningen, Piteå kommun. - 2004-3635. ; 3:1, s. 62-81
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Journal article (other academic/artistic)abstract
- Artikeln, författad av forskare, förstelärare och skolledande personal, bygger på ett samverkansprojekt med målet att utveckla en gymnasieskolas kollektiva lärande. Artikeln syftar till att bidra till förståelse av vad ett sådant utvecklingsarbete kan innebära. Metoder som användes var enkätundersökning och intervjuer. Dataanalys genomfördes med stöd av tematisk analys i olika steg (jfr Braun & Clarke, 2006) och teoretiska begrepp som kollektivt och kollegialt lärande (Larsson, 2018) samt olika former av begreppet tillit (Jederlund, 2019). Resultaten visar att förstelärare som leder utvecklingsarbete påverkas av tidigare erfarenheter från utvecklingsarbete och att det är viktigt att lärare som medverkar känner tillit till enskilda kollegor, till den större grupp de ingår i samt till processen bakom utvecklingsarbetet. Samverkansprojektet bidrog till skapande av strukturer för fortsatt utveckling samt till insikt om att utvecklingsarbete främst handlar om att utveckla arbetssätt som är lämpliga för den egna verksamheten.
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| 5. |
- Svensson, Kerstin, et al.
(author)
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A real-time PCR array for hierarchical identification of Francisella isolates
- 2009
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In: PLOS ONE. - San Fransisco : Public library of science. - 1932-6203. ; 4:12
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Journal article (peer-reviewed)abstract
- A robust, rapid and flexible real-time PCR assay for hierarchical genetic typing of clinical and environmental isolates of Francisella is presented. Typing markers were found by multiple genome and gene comparisons, from which 23 canonical single nucleotide polymorphisms (canSNPs) and 11 canonical insertion-deletion mutations (canINDELs) were selected to provide phylogenetic guidelines for classification from genus to isolate level. The specificity of the developed assay, which uses 68 wells of a 96-well real-time PCR format with a detection limit of 100 pg DNA, was assessed using 62 Francisella isolates of diverse genetic and geographical origins. It was then successfully used for typing 14 F. tularensis subsp. holarctica isolates obtained from tularemia patients in Sweden in 2008 and five more genetically diverse Francisella isolates of global origins. When applied to human ulcer specimens for direct pathogen detection the results were incomplete due to scarcity of DNA, but sufficient markers were identified to detect fine-resolution differences among F. tularensis subsp. holarctica isolates causing infection in the patients. In contrast to other real-time PCR assays for Francisella, which are typically designed for specific detection of a species, subspecies, or strain, this type of assay can be easily tailored to provide appropriate phylogenetic and/or geographical resolution to meet the objectives of the analysis.
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| 6. |
- Svensson, Kerstin, et al.
(author)
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Landscape epidemiology of tularemia outbreaks in Sweden
- 2009
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In: Emerging Infectious Diseases. - : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 15:12, s. 1937-1947
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Journal article (peer-reviewed)abstract
- Summer outbreaks of tularemia that occurred from 1995 through 2005 in 2 locations in Sweden affected 441 persons. We performed an epidemiologic investigation of these outbreaks using a novel strategy, involving high-resolution genotyping of Francisella tularensis isolates obtained from 136 patients (using 18 genetic markers developed from 6 F. tularensis genome sequences) and interviews with the patients. Strong spatial associations were found between F. tularensis subpopulations and the places of disease transmission; infection by some subpopulations occurred within areas as small as 2 km(2), indicating unidentified environmental point sources of tularemia. In both locations, disease clusters were associated with recreational areas beside water, and genetic subpopulations were present throughout the tularemia season and persisted over years. High-resolution genotyping in combination with patients' statements about geographic places of disease transmission provided valuable indications of likely sources of infection and the causal genotypes during these tularemia outbreaks.
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