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- Guo, Xiang, et al.
(author)
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Production of bacterial nanocellulose and enzyme from [AMIM]Cl-pretreated waste cotton fabrics : effects of dyes on enzymatic saccharification and nanocellulose production
- 2016
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In: Journal of chemical technology and biotechnology (1986). - : Wiley. - 0268-2575 .- 1097-4660. ; 91:5, s. 1413-1421
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Journal article (peer-reviewed)abstract
- BACKGROUND Dyed used cotton textiles is a waste material associated with environmental problems. In this study, waste dyed cotton fabrics were used as feedstock for production of bacterial nanocellulose (BNC) with Gluconacetobacter xylinus and production of enzymes with Trichoderma reesei via enzymatic saccharification.RESULTS Reactive dyes caused almost no inhibition of the cellulase activity at a concentration of 5 g L-1, but decreased the BNC production at concentrations higher than 1 g L-1. The BNC yield reached 12.8 g L-1 with cotton hydrolysate, which was 48% higher than with glucose-based medium. The spent fermentation broth after BNC harvest was subsequently utilized for enzyme production. Cellulase activities produced by T. reesei reached 5.3 U mL(-1) with spent detoxified purple bed sheet (PBS) hydrolysate, and 8.2 U mL(-1) with 2-fold diluted spent PBS hydrolysate, which was almost the same or higher than with glucose medium (5.6 U mL(-1)). The xylanase activities (60.2 U mL(-1) and 88.0 U mL(-1)) obtained with the two media were 3-4 times higher than that obtained with glucose medium (21.0 U mL(-1)).CONCLUSION This approach could contribute to economical conversion of cellulosic waste to two high value-added microbial products, while also providing new raw materials for a more sustainable textile industry.
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| 2. |
- Poplawski, Andrzej B., et al.
(author)
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Frequent genetic differences between matched primary and metastatic breast cancer provide an approach to identification of biomarkers for disease progression
- 2010
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In: European Journal of Human Genetics. - : Springer Science and Business Media LLC. - 1018-4813 .- 1476-5438. ; 18:5, s. 560-568
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Journal article (peer-reviewed)abstract
- Breast cancer is a major cause of morbidity and mortality in women and its metastatic spread is the principal reason behind the fatal outcome. Metastasis-related research of breast cancer is however underdeveloped when compared with the abundant literature on primary tumors. We applied an unexplored approach comparing at high resolution the genomic profiles of primary tumors and synchronous axillary lymph node metastases from 13 patients with breast cancer. Overall, primary tumors displayed 20% higher number of aberrations than metastases. In all but two patients, we detected in total 157 statistically significant differences between primary lesions and matched metastases. We further observed differences that can be linked to metastatic disease and there was also an overlapping pattern of changes between different patients. Many of the differences described here have been previously linked to poor patient survival, suggesting that this is a viable approach toward finding biomarkers for disease progression and definition of new targets useful for development of anticancer drugs. Frequent genetic differences between primary tumors and metastases in breast cancer also question, at least to some extent, the role of primary tumors as a surrogate subject of study for the systemic disease. European Journal of Human Genetics (2010) 18, 560-568; doi:10.1038/ejhg.2009.230; published online 6 January 2010
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