| 1. |
- Blom, Hans, et al.
(author)
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Parallel Flow Measurements in Microstructures by Use of a Multifocal 4 x 1 Diffractive Optical Fan-Out Element
- 2002
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In: Applied Optics. - 1559-128X .- 2155-3165. ; 41:31, s. 6614-6620
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Journal article (peer-reviewed)abstract
- We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 μm x 20 μm square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied.
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| 2. |
- Blom, Hans, et al.
(author)
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Parallel Fluorescence Detection of Single Biomolecules in Microarrays by a Diffractive-Optical-Designed 2 x 2 Fan-Out Element
- 2002
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In: Applied Optics. - 1559-128X .- 2155-3165. ; 41:16, s. 3336-3342
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Journal article (peer-reviewed)abstract
- We have developed a multifocal diffractive-optical fluorescence correlation spectroscopy system for parallel excitation and detection of single tetramethylrhodamine biomolecules in microarrays. Multifocal excitation was made possible through the use of a 2 × 2 fan-out diffractive-optical element with uniform intensity in all foci. Characterization of the 2 × 2 fan-out diffractive-optical element shows formation of almost perfect Gaussian foci of submicrometer lateral diameter, as analyzed by thermal motion of tetramethylrhodamine dye molecules in solution. Results of parallel excitation and detection in a high-density microarray of circular wells show single-biomolecule sensitivity in all four foci simultaneously.
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| 3. |
- Ekberg, Mats, 1961, et al.
(author)
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Multilevel phase holograms manufactured by electron-beam lithography
- 1990
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In: Optics Letters. - 0146-9592 .- 1539-4794. ; 15, s. 568-569
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Journal article (peer-reviewed)abstract
- Ten-level transmission phase holograms (kinoforms) manufactured in one resist layer by electron-beam lithography are reported for the first time to the authors' knowledge. The measured hologram diffraction efficiencies were 70% for the two resist materials used. This corresponds to 82% of the maximum theoretical value for these holograms and is, to the authors' knowledge, the highest reported to date
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| 4. |
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| 6. |
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| 7. |
- Engström, David, 1976, et al.
(author)
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Beam steering with four-level phase-modulating SLM
- 2002
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In: in OSA TOPS, Vol 75, Diffractive Optics and Micro-Optics, OSA Technical Digest, Postconference Edition. ; 75, s. 14-16
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Conference paper (other academic/artistic)
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| 8. |
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| 9. |
- Gosch, M., et al.
(author)
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Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
- 2005
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In: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 10:5
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Journal article (peer-reviewed)abstract
- Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 X 2 fan-out diffractive optical elements (DOES), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 x 2 fiber arrays.
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| 10. |
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